LOCUS       sequence-237552-       12827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of disordered protein BRD4(462-1362), fluorescent 
            protein mCherry, and sspB which upon light activation binds to 
            iLID..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-BRD4-Delta-N-mCherry-sspB
FEATURES             Location/Qualifiers
     source          1..12827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             942..959
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3564..3893
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    4000..4588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4053..4073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4471..4482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4591..4607)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4592..4608)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4698..4931
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4956..4974)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4957..4974)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5264..5283)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5497..5516)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5616..5638
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5676..5694)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5762..5866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5867..5935,5936..6727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6085..6104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6898..7486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7387..7406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7640..7657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7727..7747)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7732..8061
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7912..8047
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9195..9260
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9390..9410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9835..9969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9872..9891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9926..9945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10137..10770
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10817..10942
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11439..11672
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11857..11901
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12186..12303
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(12456..12827,1..36)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 ggcgcgccag tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt
       61 ctcacgcgtc aagtggagca aggcaggtgg acagtggatc cggagctacc atggaggagc
      121 cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg gccccgccct
      181 catccagcga cagcagcagc gatagctcct cggacagtga cagttcgact gatgactctg
      241 aggaggagcg agcccagcgg ctggctgagc tccaggagca gctcaaagcc gtgcacgagc
      301 agcttgcagc cctctctcag ccccagcaga acaaaccaaa gaaaaaggag aaagacaaga
      361 aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt ggaagagaat aaaaaaagca
      421 aagccaagga acctcctcct aaaaagacga agaaaaataa tagcagcaac agcaatgtga
      481 gcaagaagga gccagcgccc atgaagagca agccccctcc cacgtatgag tcggaggaag
      541 aggacaagtg caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca
      601 agctccccgg cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc
      661 tgaagaattc caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac
      721 tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg
      781 agaaagttga tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg
      841 agagctccag tgagtccagc tcctctgaca gcgaagactc cgaaacagag atggctccga
      901 agtcaaaaaa gaaggggcac cccgggaggg agcagaagaa gcaccatcat caccaccatc
      961 agcagatgca gcaggccccg gctcctgtgc cccagcagcc gcccccgcct ccccagcagc
     1021 ccccaccgcc tccacctccg cagcagcaac agcagccgcc acccccgcct cccccaccct
     1081 ccatgccgca gcaggcagcc ccggcgatga agtcctcgcc cccacccttc attgccaccc
     1141 aggtgcccgt cctggagccc cagctcccag gcagcgtctt tgaccccatc ggccacttca
     1201 cccagcccat cctgcacctg ccgcagcctg agctgccccc tcacctgccc cagccgcctg
     1261 agcacagcac tccaccccat ctcaaccagc acgcagtggt ctctcctcca gctttgcaca
     1321 acgcactacc ccagcagcca tcacggccca gcaaccgagc cgctgccctg cctcccaagc
     1381 ccgcccggcc cccagccgtg tcaccagcct tgacccaaac acccctgctc ccacagcccc
     1441 ccatggccca acccccccaa gtgctgctgg aggatgaaga gccacctgcc ccacccctca
     1501 cctccatgca gatgcagctg tacctgcagc agctgcagaa ggtgcagccc cctacgccgc
     1561 tactcccttc cgtgaaggtg cagtcccagc ccccaccccc cctgccgccc ccaccccacc
     1621 cctctgtgca gcagcagctg cagcagcagc cgccaccacc cccaccaccc cagccccagc
     1681 ctccacccca gcagcagcat cagccccctc cacggcccgt gcacttgcag cccatgcagt
     1741 tttccaccca catccaacag cccccgccac cccagggcca gcagcccccc catccgcccc
     1801 caggccagca gccacccccg ccgcagcctg ccaagcctca gcaagtcatc cagcaccacc
     1861 attcaccccg gcaccacaag tcggacccct actcaaccgg tcacctccgc gaagccccct
     1921 ccccgcttat gatacattcc ccccagatgt cacagttcca gagcctgacc caccagtctc
     1981 caccccagca aaacgtccag cctaagaaac aggagctgcg tgctgcctcc gtggtccagc
     2041 cccagcccct cgtggtggtg aaggaggaga agatccactc acccatcatc cgcagcgagc
     2101 ccttcagccc ctcgctgcgg ccggagcccc ccaagcaccc ggagagcatc aaggcccccg
     2161 tccacctgcc ccagcggccg gaaatgaagc ctgtggatgt cgggaggcct gtgatccggc
     2221 ccccagagca gaacgcaccg ccaccagggg cccctgacaa ggacaaacag aaacaggagc
     2281 cgaagactcc agttgcgccc aaaaaggacc tgaaaatcaa gaacatgggc tcctgggcca
     2341 gcctagtgca gaagcatccg accaccccct cctccacagc caagtcatcc agcgacagct
     2401 tcgagcagtt ccgccgcgcc gctcgggaga aagaggagcg tgagaaggcc ctgaaggctc
     2461 aggccgagca cgctgagaag gagaaggagc ggctgcggca ggagcgcatg aggagccgag
     2521 aggacgagga tgcgctggag caggcccggc gggcccatga ggaggcacgt cggcgccagg
     2581 agcagcagca gcagcagcgc caggagcaac agcagcagca gcaacagcaa gcagctgcgg
     2641 tggctgccgc cgccacccca caggcccaga gctcccagcc ccagtccatg ctggaccagc
     2701 agagggagtt ggcccggaag cgggagcagg agcgaagacg ccgggaagcc atggcagcta
     2761 ccattgacat gaatttccag agtgatctat tgtcaatatt tgaagaaaat cttttcggtg
     2821 gaggaatggt gtctaaaggc gaggaggata acatggctat aatcaaggaa tttatgaggt
     2881 tcaaggtgca tatggaggga tctgtgaacg gtcacgagtt cgaaatcgag ggcgaaggcg
     2941 aggggcgacc ctatgaaggg acacagacgg ccaaactgaa ggtgaccaag ggtggccccc
     3001 tgcctttcgc ctgggacatc ctgtcacccc agtttatgta cggctctaag gcttacgtta
     3061 agcaccctgc cgatattccg gactacttga aactgagctt ccctgaaggg ttcaagtggg
     3121 aacgggtaat gaattttgag gatggcggtg tagtcacagt tacccaggac agctctcttc
     3181 aggacggaga atttatctat aaggttaaac ttcggggcac taacttccca tcagacggcc
     3241 ccgtgatgca gaagaaaact atgggctggg aagccagctc agaacgcatg tatcccgagg
     3301 acggagcact gaagggagaa atcaagcagc gactcaagct gaaggatggg ggacattatg
     3361 atgctgaggt caaaaccacc tataaggcca agaaacccgt tcagcttcct ggggcataca
     3421 acgtgaatat caaactggat attacatccc ataacgaaga ctataccatc gtggaacagt
     3481 acgagcgggc cgagggcaga catagcacag ggggcatgga tgaattgtac aaggcggccg
     3541 gatccggtgg tacctctgga ggtagctccc cgaaacgccc taagctgctg cgtgaatatt
     3601 acgattggct ggttgataac agctttaccc catatctggt ggtggatgcc acatacctgg
     3661 gcgtgaacgt gcccgtggag tatgtgaaag acggtcagat cgtgctgaat ctgtctgcaa
     3721 gtgcgaccgg caacctgcaa ctgacaaatg attttatcca gttcaacgcc cgctttaagg
     3781 gcgtgtctcg tgaactgtat atcccgatgg gtgccgctct ggccatttac gctcgcgaga
     3841 acggcgatgg tgtgatgttc gaaccagaag aaatctatga cgagctgaat attggttaag
     3901 gatcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc
     3961 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     4021 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4081 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4141 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4201 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4261 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4321 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4381 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4441 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4501 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4561 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     4621 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4681 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4741 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4801 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4861 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4921 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     4981 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     5041 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     5101 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     5161 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     5221 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     5281 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     5341 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     5401 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     5461 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     5521 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5581 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5641 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5701 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5761 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6481 aatagactgg atggaggcgg ataaagttgc aggaacactt ctgcgctcgg cccttccggc
     6541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7561 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7621 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7681 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     7741 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7801 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7861 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7921 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7981 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8041 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     8101 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     8161 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     8221 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     8281 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     8341 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     8401 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     8461 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     8521 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     8581 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     8641 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     8701 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     8761 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     8821 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     8881 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     8941 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     9001 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     9061 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     9121 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     9181 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     9241 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     9301 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     9361 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     9421 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     9481 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     9541 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     9601 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     9661 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     9721 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9781 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9961 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10021 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10081 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
    10141 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
    10201 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
    10261 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
    10321 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
    10381 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
    10441 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
    10501 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
    10561 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
    10621 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10681 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    10741 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
    10801 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10861 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10921 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    10981 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    11041 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    11101 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    11161 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    11221 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    11281 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    11341 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    11401 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    11461 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    11521 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    11581 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    11641 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    11701 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    11761 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    11821 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    11881 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    11941 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    12001 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    12061 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    12121 gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc
    12181 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
    12241 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
    12301 tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa
    12361 ttcctgcagc cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
    12421 aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga
    12481 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
    12541 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
    12601 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
    12661 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
    12721 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
    12781 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactc
//
