LOCUS       sequence-237555-        6131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RING finger protein 34 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Jin H, Chiou TT, Serwanski DR, Miralles CP, Pinal N, De Blas AL
  TITLE     Ring finger protein 34 (RNF34) interacts with and promotes 
            gamma-aminobutyric acid type-A receptor degradation via 
            ubiquitination of the gamma2 subunit.
  JOURNAL   J Biol Chem. 2014 Oct 17;289(42):29420-36. doi: 
            10.1074/jbc.M114.603068. Epub 2014 Sep 5.
  PUBMED    25193658
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.603068"; journalName: "J Biol Chem"; date: 
            "2014-10-17- 17"; volume: "289"; issue: "42"; pages: "29420-36"
COMMENT     Sequence Label: RNF34
FEATURES             Location/Qualifiers
     source          1..6131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          287..1303
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1227..1249
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1311..1330
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2505..2524)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2570..2625
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2624..2643)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2986..3002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2986..3002)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2999..3021)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3010..3026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3034..3040,3041..3058,3059..3064))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3079..3100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3159..3354
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3205..3340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3360..3494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3397..3416)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3451..3470
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3562..3579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3733..4321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3813..3832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4492..5283,5284..5352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5115..5134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5353..5457)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5488..5867
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 rrssssscgg ggggggggsc cccccttctc byyccccccc cccccccccc cccccccccc
       61 ccccccmwtt tttttttttt ttttttttta attttttkgg gggggggggg gggggggggg
      121 gggggggggg cgcgcgccgg gsggggcggg gcggggcgag gggcggggcg gggcgaggcg
      181 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
      241 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
      301 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      361 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      421 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
      481 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
      541 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
      601 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
      661 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
      721 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
      781 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
      841 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
      901 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
      961 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     1021 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     1081 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     1141 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     1201 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     1261 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1321 ggttattgtg ctgtctcatc attttggcaa agaattcatg aaggcgggtg ctacctctat
     1381 gtgggcttca tgctgtggac tgctgaatga agtcatggga actggagctg tcagaggcca
     1441 gcaggcagga ttcccaggaa gcactggtcc attcaggttt acaccgagtt ctgactttcc
     1501 tacctaccca ccagcagcta cagaaggacc caatatagtt tgcaaagcct gtggactttc
     1561 attttcagtc tttagaaaga agcacgtctg ctgtgactgc aagaaggact tttgctccct
     1621 ttgttcggtc tcacaagaaa acctccgcag atgctctaca tgtcacctac tccaagagac
     1681 agccttccag cgccctcagt tgatgagact aaaagtgaag gacctgcggc agtatctgct
     1741 cctcagaaac gtaccgaccg acacttgtcg cgagaaggag gacttagtgg atctagtgct
     1801 gtgccatcgt ggactgggct cgggggatgg cctggactca aggagtctca gttcctcaag
     1861 atcccagact tctagttttt ttacacaatc ctatttttca aattatacac ccccctctgc
     1921 tactgtgtcc tccttccagg gagagctgat ggacagagag ggcaccttca ggtcagaagt
     1981 tctcacacag gtacaaagtg aattagcttc agcaaacacg gatgatgagg atggggagga
     2041 agatgacgac gatgatgacg acgacgacga cgaggatgac gatgaacaag aagaaaactt
     2101 agaggaacag aacctggggc tctctaagaa gaaggcgaga gcttccctgt ccgacctgtc
     2161 aagccttgaa gaggtggaag ggatgagcgt gcgccagctg aaggaaatcc tggctcggaa
     2221 ttttgtcaac tattctggct gctgtgaaaa gtgggagctg gtggagaaag tcaaccggct
     2281 gtacaaggag aatgaggaaa accaaaagtc atatggtgag cggatgcaac ttcaggatga
     2341 ggaagacgac agcctgtgcc gaatctgcat ggatgcggtc attgactgtg tcctgctgga
     2401 gtgtgggcac atggtcacct gcaccaagtg cggcaagcgc atgagtgagt gtcccatctg
     2461 caggcagtat gtggttcgag ctgtgcatgt tttcaagtcc tagatctttt tccctctgcc
     2521 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     2581 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2641 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     2701 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     2761 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2821 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2881 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2941 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     3001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3121 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     3181 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3241 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3301 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     3361 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3421 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3481 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3541 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3721 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3781 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3841 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3901 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3961 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4021 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4081 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4141 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4201 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4261 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4321 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4381 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4441 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4501 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4561 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4621 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4681 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4741 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4801 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4861 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4921 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4981 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5041 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5101 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5161 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5221 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5281 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5341 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5401 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5461 atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat
     5521 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5581 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5641 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5701 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5761 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     5821 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
     5881 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     5941 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg gcgcgcccsg
     6001 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggggggggcc
     6061 rrymrrgggg gggggccccs rargtttcyt tttttkgggg gggggggggg gggggggsss
     6121 ykramaamrs k
//
