LOCUS       sequence-237559-        5745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of LisDPS in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Allen M, Willits D, Young M, Douglas T
  TITLE     Constrained synthesis of cobalt oxide nanomaterials in the 
            12-subunit protein cage from Listeria innocua.
  JOURNAL   Inorg Chem. 2003 Oct 6;42(20):6300-5. doi: 10.1021/ic0343657.
  PUBMED    14514305
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Inorg Chem"; date: "2003-10-6- 6"; volume: "42"; issue: "20"; 
            pages: "6300-5"
COMMENT     Sequence Label: LisDPS
FEATURES             Location/Qualifiers
     source          1..5745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     67..86
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     174..193
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        174..192
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    193..217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             779..796
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(849..867)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      863..910
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      947..1402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1034..1053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1244..1265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1494..2309)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1494..2309)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2217..2236
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2301..2320
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2431..3019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2920..2939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3173..3190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3205..3344
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3270..3289)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3430..3452
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3446..3637)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4446..5528)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5490..5509
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5529..5606)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
       61 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      121 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg
      181 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatataca tatgaaaaca atcaactcag tagacacaaa ggaatttttg
      301 aatcatcaag tagcgaattt aaacgtattc acagtaaaaa ttcatcaaat tcattggtat
      361 atgagaggcc acaacttctt cactttacat gaaaaaatgg atgatttata tagcgaattc
      421 ggtgaacaaa tggatgaagt agcagaacgt ttacttgcca ttggtggaag cccattctcg
      481 actttaaaag agtttttaga aaatgcgagt gtagaagaag ctccttatac aaaacctaaa
      541 actatggatc aattaatgga agacttagtt ggtacattag aattacttag agacgaatat
      601 aaacaaggca ttgagctaac tgacaaagaa ggcgacgatg taacaaacga tatgctaatt
      661 gcatttaaag ctagcattga caaacatatc tggatgttca aagcattcct tggaaaagct
      721 ccattagaat aaggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      781 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      841 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      901 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1261 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1321 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1381 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1441 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     1501 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     1561 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     1621 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     1681 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     1741 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     1801 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     1861 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     1921 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     1981 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     2041 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     2101 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     2161 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     2221 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     2281 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     2341 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3181 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3241 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3301 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     3361 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3421 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3481 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     3541 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     3601 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     3661 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     3721 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     3781 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     3841 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
     3901 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
     3961 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
     4021 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
     4081 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc
     4141 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
     4201 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
     4261 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
     4321 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     4381 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     4441 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4501 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     4561 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     4621 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     4681 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     4741 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     4801 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     4861 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     4921 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     4981 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     5041 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     5101 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     5161 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     5221 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     5281 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     5341 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     5401 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     5461 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     5521 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     5581 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     5641 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     5701 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcc
//
