LOCUS       sequence-237562-       10763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N terminal domain of FUS (1-214) tagged with SspB and mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher 
            JE, Brangwynne CP
  TITLE     Mapping Local and Global Liquid Phase Behavior in Living Cells Using
            Photo-Oligomerizable Seeds.
  JOURNAL   Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 
            10.1016/j.cell.2018.10.048.
  PUBMED    30500534
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1467-1480.e13"
COMMENT     Sequence Label: M23: pHR-SFFVp-FUSN::mCherry::SspB
FEATURES             Location/Qualifiers
     source          1..10763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             491..502
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1500..1829
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    1936..2524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1989..2009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2407..2418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2527..2543)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2528..2544)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2634..2867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2892..2910)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2893..2910)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3200..3219)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3433..3452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3552..3574
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3612..3630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3698..3802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3803..3871,3872..4663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4021..4040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4834..5422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5323..5342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5576..5593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5663..5683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5668..5997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5848..5983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7131..7196
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7326..7346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7771..7905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7808..7827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7862..7881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8073..8706
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8753..8878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9375..9608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9793..9837
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10122..10239
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10392..10763,1..36)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 ggcgcgccag tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt
       61 ctcacgcgtc aagtggagca aggcaggtgg acagtggatc cggagctacc atggcctcaa
      121 acgattatac ccaacaagca acccaaagct atggggccta ccccacccag cccgggcagg
      181 gctattccca gcagagcagt cagccctacg gacagcagag ttacagtggt tatagccagt
      241 ccacggacac ttcaggctat ggccagagca gctattcttc ttatggccag agccagaaca
      301 caggctatgg aactcagtca actccccagg gatatggctc gactggcggc tatggcagta
      361 gccagagctc ccaatcgtct tacgggcagc agtcctccta tcctggctat ggccagcagc
      421 cagctcccag cagcacctcg ggaagttacg gtagcagttc tcagagcagc agctatgggc
      481 agccccagag tgggagctac agccagcagc ctagctatgg tggacagcag caaagctatg
      541 gacagcagca aagctataat ccccctcagg gctatggaca gcagaaccag tacaacagca
      601 gcagtggtgg tggaggtgga ggtggaggtg gaggtaacta tggccaagat caatcctcca
      661 tgagtagtgg tggtggcagt ggtggcggtt atggcaatca agaccagagt ggtggaggtg
      721 gcagcggtgg ctatggacag caggaccgtg gaggtggagg aatggtgtct aaaggcgagg
      781 aggataacat ggctataatc aaggaattta tgaggttcaa ggtgcatatg gagggatctg
      841 tgaacggtca cgagttcgaa atcgagggcg aaggcgaggg gcgaccctat gaagggacac
      901 agacggccaa actgaaggtg accaagggtg gccccctgcc tttcgcctgg gacatcctgt
      961 caccccagtt tatgtacggc tctaaggctt acgttaagca ccctgccgat attccggact
     1021 acttgaaact gagcttccct gaagggttca agtgggaacg ggtaatgaat tttgaggatg
     1081 gcggtgtagt cacagttacc caggacagct ctcttcagga cggagaattt atctataagg
     1141 ttaaacttcg gggcactaac ttcccatcag acggccccgt gatgcagaag aaaactatgg
     1201 gctgggaagc cagctcagaa cgcatgtatc ccgaggacgg agcactgaag ggagaaatca
     1261 agcagcgact caagctgaag gatgggggac attatgatgc tgaggtcaaa accacctata
     1321 aggccaagaa acccgttcag cttcctgggg catacaacgt gaatatcaaa ctggatatta
     1381 catcccataa cgaagactat accatcgtgg aacagtacga gcgggccgag ggcagacata
     1441 gcacaggggg catggatgaa ttgtacaagg cggccggatc cggtggtacc tctggaggta
     1501 gctccccgaa acgccctaag ctgctgcgtg aatattacga ttggctggtt gataacagct
     1561 ttaccccata tctggtggtg gatgccacat acctgggcgt gaacgtgccc gtggagtatg
     1621 tgaaagacgg tcagatcgtg ctgaatctgt ctgcaagtgc gaccggcaac ctgcaactga
     1681 caaatgattt tatccagttc aacgcccgct ttaagggcgt gtctcgtgaa ctgtatatcc
     1741 cgatgggtgc cgctctggcc atttacgctc gcgagaacgg cgatggtgtg atgttcgaac
     1801 cagaagaaat ctatgacgag ctgaatattg gttaaggatc ggccgcaact cccacctgca
     1861 acatgcgtga ctgactgagg ccgcgactct agagtcgacc tgcaggcatg caagcttgat
     1921 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1981 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2041 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2101 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2161 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2221 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2281 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2341 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2401 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2461 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2521 ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat
     2581 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     2641 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     2701 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2761 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2821 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     2881 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     2941 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     3001 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     3061 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     3121 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     3181 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     3241 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     3301 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     3361 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     3421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3481 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3541 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3601 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3661 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3781 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3841 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3901 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3961 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4021 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4081 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4141 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4201 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4261 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4321 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4381 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4441 agttgcagga acacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4501 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4561 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4621 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4681 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4981 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5521 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5581 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5641 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5701 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5761 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5821 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5881 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5941 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6001 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     6061 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     6121 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     6181 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     6241 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     6301 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     6361 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     6421 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     6481 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     6541 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
     6601 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     6661 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
     6721 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     6781 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     6841 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     6901 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
     6961 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
     7021 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
     7081 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     7141 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     7201 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     7261 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     7321 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     7381 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     7441 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     7501 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     7561 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     7621 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     7681 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     7741 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     7801 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7861 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     7921 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     7981 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     8041 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     8101 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     8161 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     8221 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     8281 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     8341 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     8401 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     8461 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     8521 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8581 agccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8701 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8761 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8821 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8881 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8941 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9001 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9061 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     9121 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     9181 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     9241 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9301 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9361 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9421 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9481 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9541 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9601 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9661 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     9721 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     9781 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     9841 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     9901 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     9961 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    10021 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    10081 ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg
    10141 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
    10201 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
    10261 agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa
    10321 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
    10381 agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga
    10441 agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc
    10501 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
    10561 cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa
    10621 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
    10681 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
    10741 aaagagctca caacccctca ctc
//
