LOCUS       sequence-237566-       11294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; 
            note plasmid contains 24xGCN4 repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA
  TITLE     Single-Molecule Imaging of mRNA Localization and Regulation during 
            the Integrated Stress Response.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 
            10.1016/j.molcel.2018.12.006.
  PUBMED    30661979
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 
            10.1016/j.molcel.2018.12.006."
COMMENT     Sequence Label: pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP ...
FEATURES             Location/Qualifiers
     source          1..11294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3710..3757)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    join(3869..3887,3888..3910,3911..3929,3930..3952,3953..3971,
                     3972..3994,3995..4013,4014..4036,4037..4055,4056..4078,
                     4079..4097,4098..4120,4121..4139)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4172..4210
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4286..4418
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4401..4420
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4463..4481
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4485..4494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4560..4616
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4632..4688
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4704..4760
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4776..4832
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4848..4904
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4920..4976
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4992..5048
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5064..5120
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5136..5192
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5208..5264
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5280..5336
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5352..5408
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5424..5480
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5496..5552
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5568..5624
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5640..5696
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5712..5768
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5784..5840
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5856..5912
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5928..5984
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6000..6056
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6072..6128
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6216..6272
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6306..7235
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(7236..7238,7239..7559)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             7239..7562
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    9092..9226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9170..9189
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9334..9367)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9420..9436)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9452..9474)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9885..10242
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10307..10776
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca atcacacaca
     2821 cacaatcaca cacaaacata catacacaca atcacacaca accatacaca catatacaca
     2881 atcacacaca caaacacaat cacacacaca cacatcaatc aatcaatcaa tcaatatacc
     2941 aaaaatgata gctcatacct gtaatcttag atgtgggaag gctaaaacag caggctaccc
     3001 atgacttcaa ggtcaccctg ggttacagag tagctaatag tctagcttga actatagtgt
     3061 aggatcttta aaaaaaaatt aaccaaccaa ccaacaaaca aacaaacaaa caacaacaac
     3121 aacaaatgga agacagagga caaaaaaaaa taaaagaaaa agtagaaagc caatggagat
     3181 actatcttaa aattagaaat atttatgaca cattctgaat actccatgtt tatttcaagg
     3241 aagccaatgt aactttaagt actatggcca tttaatgtaa atacttaaga aaaaaaacca
     3301 aattaatttt gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca
     3361 gaggagaccc tctcaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     3421 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     3481 gttgtgggct cgagctcggg gagcagaagc gcgcgaacag aagtcgagct agcttgccaa
     3541 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
     3601 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
     3661 cctggaccac tgatatcctg tctttaacaa attggactaa tccggatctg aagttcctat
     3721 actttctaga gaataggaac ttcggaatag gaacttcgcg gccagatctt ttgcggccgc
     3781 tttgggccgg ccgaatgaat tcgcgcaaat ttaaccctca ctaaagggaa caaaagctgg
     3841 gtaccgggcc ccccctcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     3901 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     3961 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     4021 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     4081 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     4141 aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat
     4201 aagcagagct cgtttaggcc accgtcctct cttcctcggc gctgcctacg gaggtggcag
     4261 ccatctcctt ctcggcaccg gtcaggtaag tatcaaggtt ccaagacagg tttaaggaga
     4321 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     4381 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     4441 cagctcttaa ggctagagta cttaatacga ctcactatag gctagccacc atgggcgcca
     4501 tcatacacgc ggccgcaatc atcggtccgg gtggatctgg aggtggaggt tctggaggag
     4561 aagaactttt gagcaagaat tatcatcttg agaacgaagt ggctcgtctt aagaaaggtt
     4621 ctggcagtgg agaagaactg ctttcaaaga attaccacct ggaaaatgag gtagctagac
     4681 tgaaaaaggg gagcggaagt ggggaggagt tgctgagcaa aaattatcat ttggagaacg
     4741 aagtagcacg actaaagaaa gggtccggat cgggtgagga gttactctcg aaaaattatc
     4801 atctcgaaaa cgaagtggct cggctaaaaa agggcagtgg ttctggagaa gagctattat
     4861 ctaaaaacta ccacctcgaa aatgaggtgg cacgcttaaa aaagggaagt ggcagtggtg
     4921 aagagctact atccaagaat tatcatcttg agaacgaggt agcgcgtttg aagaagggtt
     4981 ccggctcagg agaggaactg ctctcgaaga actatcatct tgaaaatgag gtcgctcgat
     5041 taaaaaaggg atcgggcagt ggtgaggaac tactttcaaa gaattaccac ctcgaaaacg
     5101 aagtagctcg attaaagaaa ggttcagggt cgggtgaaga attactgagt aaaaattatc
     5161 atctggaaaa tgaggtagcg agactaaaaa aggggagtgg ttctggcgaa gagttgctat
     5221 cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa aaagggctca ggctcaggcg
     5281 aggagttgct ctcgaaaaac taccacttgg aaaatgaggt cgcgaggttg aaaaagggga
     5341 gcgggtcggg cgaggagtta ttgagcaaaa actatcattt agagaacgaa gtcgcgcgct
     5401 taaagaaagg ctcgggctcg ggcgaagaac tcttatcgaa gaactaccac ctcgaaaatg
     5461 aggtcgccag gttgaaaaag ggcagtggca gcggggagga actcttgagc aagaactacc
     5521 acttggagaa tgaggtcgcg agattgaaga aagggtcggg gagcggcgag gaattgctca
     5581 gcaagaatta tcatttggag aacgaagtcg ccaggctcaa gaaaggctcg gggtcggggg
     5641 aggaattgtt gagtaaaaac taccacttgg aaaatgaagt cgccaggctc aaaaaaggga
     5701 gtgggagcgg cgaagagtta ttgagcaaaa attaccactt ggagaacgaa gtggcaaggc
     5761 tcaagaaagg gagcggcagc ggggaggagc tcttatcgaa gaactaccac ttagagaatg
     5821 aagtcgcccg cttgaagaaa ggctcgggga gcggggaaga gctcttgagc aagaactacc
     5881 acttggaaaa tgaggtggcg cgcttgaaga aagggagcgg gagcggggaa gagttactat
     5941 ctaagaatta tcatctcgag aacgaggtgg ctcgactaaa gaagggctcc ggcagtgggg
     6001 aggaactcct gtcgaagaac tatcatcttg aaaatgaggt tgcaagactt aaaaaggggt
     6061 ccggatcagg tgaggaacta ctcagtaaga attaccacct ggaaaacgaa gtagctcgat
     6121 taaagaaagg ttcagggtcg ggtgaagaac tgctctcaaa agattatcat ttggaaaatg
     6181 aggttgcacg tttaaaaaag ggaagtggca gtggtgagga acttctgtcg aaaaattatc
     6241 atctcgagaa tgaagtagcc cgacttaaaa agggttctgg ctcgggtcag cggccgcacc
     6301 ggtcagcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt
     6361 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg
     6421 agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt
     6481 ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg
     6541 gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt
     6601 acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg
     6661 actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag atcaaggcca
     6721 tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg
     6781 aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact
     6841 tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg
     6901 ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc
     6961 ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact
     7021 acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag tccgaccctg
     7081 ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga
     7141 aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt aagtacatca
     7201 agagcttcgt ggagcgcgtg ctgaagaacg agcagatggg agtgcaggtg gaaaccatct
     7261 ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgtgtggtg cactacaccg
     7321 ggatgcttga agatggaaag aaagtcgatt cctcccggga cagaaacaag ccctttaagt
     7381 ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg
     7441 tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc
     7501 caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta aaaccggaat
     7561 aagggccagg gaagaactgg ctcggaaaga ggaccccaca tgttgtagct tcaagtttaa
     7621 acggatccaa cctacaaacg ggtggaggat caccccaccc gacacttcac aatcaagggg
     7681 tacaatacac aagggtggag gaacacccca ccctccagac acattacaca gaaatccaat
     7741 caaacagaag caccatcagg gcttctgcta ccaaatttat ctcaaaaaac tacaacaagg
     7801 aatcaccatc agggattccc tgtgcaatat acgtcaaacg agggccacga cgggaggacg
     7861 atcacgcctc ccgaatatcg gcatgtctgg ctttcgaatt cagtgcgtgg agcatcagcc
     7921 cacgcagcca atcagagtcg aatacaagtc gactttcgcg aagagcatca gccttcgcgc
     7981 cattcttaca caaaccacac tctcccctac aggaacagca tcagcgttcc tgcccagtac
     8041 ccaactcaag aaaatttatg tccccatgca gcatcagcgc atgggcccca agaatacatc
     8101 cccaacaaaa tcacatccga gcaccaacag ggctcggagt gttgtttctt gtccaactgg
     8161 acaaaccctc catggaccat caggccatgg actctcacca acaagacaaa aactactctt
     8221 ctcgaagcag catcagcgct tcgaaacact cgagcataca ttgtgcctat ttcttgggtg
     8281 gacgatcacg ccacccatgc tctcacgaat ttcaaaacac ggacaaggac gagcaccacc
     8341 agggctcgtc gttccacgtc caatacgatt acttaccttt cgggatcacg atcacggatc
     8401 ccgcagctac atcacttcca ctcaggacat tcaagcatgc acgatcacgg catgctccac
     8461 aagtctcaac cacagaaact accaaatggg ttcagcacca gcgaacccac tcctacctca
     8521 aacctcttcc cacaaaactg gcaagcagga tcaccgcttg cccattccaa cataccaaat
     8581 caaaaacaat tactggtaca gcatcagcgt accagcccac atctctcact actatcaaaa
     8641 accaaaccgt tcagcaacag cgaacggtac acacggaaaa atcaactggt ttacaaatac
     8701 gaaagacgat cacgctttcg tccagcgcaa actattacga aaaacatccg acgggaagag
     8761 caacagcctt cccgcggcgg aaaacctcac aaaaacacga caaacggatg cacgaacacg
     8821 gcatccgccg acaacccaca aacttacaac caggcaaacg gtgcaggatc accgcaccgt
     8881 acatcaaaca cctcagatct catttcgcgc gggatccaga ccacctcccc tgcgagctaa
     8941 gctggacagc caatgacggg taagagagtg acatttttca ctaacctaag acaggagggc
     9001 cgtcagagct actgcctaat ccaaagacgg gtaaaagtga taaaaatgta tcactccaac
     9061 ctaagacagg cgcagcttcc gagggatttg agatccagac atgataagat acattgatga
     9121 gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga
     9181 tgctattgcc ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     9241 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     9301 cctctacaaa tgtggtatgg ctgattatga tctgaagttc ctatactatt tgaagaatag
     9361 gaacttcgga ataggaactt cggatctcat atggccatat gggggcgcct agagaaggag
     9421 tgagggctgg ataaagggag gatcgaggcg gggtcgaacg aggaggttca agggggagag
     9481 acggggcgga tggaggaaga ggaggcggag gcttagggtg tacaaagggc ttgacccagg
     9541 gaggggggtc aaaagccaag gcttcccagg tcacgatgta ggggacctgg tctgggtgtc
     9601 catgcgggcc aggtgaaaag accttgatct taacctgggt gatgaggtct cggttaaagg
     9661 tgccgtctcg cggccatccg acgttaaagg ttggccattc tgcagagcag aaggtaaccc
     9721 aacgtctctt cttgacatct accgactggt tgtgagcgat ccgctcgaca tctttccagt
     9781 gacctaaggt caaacttaag ggagtggtaa caggctagcc cgggcccata ttttcagaca
     9841 aatacagaaa cacagtcaga cagagacaac acagaacgat gctgcagcag acaagacgcg
     9901 cggcgcggct tcggtcccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     9961 ggttctcgtc tcctaccaga accacatatc cctcctctaa ggggggtgca ccaaagagtc
    10021 ctaaacgatc gggattttag gactcaggtc gggccacaaa aacggccccc gaagtccctg
    10081 ggacgtctcc cagggttgcg gccgggtgtt ccgaactcgt cagttccacc acgggtccgc
    10141 cagatacaga gctagttagc taactagtac cgacgcaggc gcataaaatc agtcatagac
    10201 actagacaat cggacagaca cagataagtt gctggccagc ttacctcccg gtggtgggtc
    10261 ggtggtccct gggcaggggt ctcccgatcc cggacgagcc cccaaatgaa agacccccgc
    10321 tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac cacaagtcgg
    10381 atgcaactgc aagagggttt attggataca cgggtacccg ggcgactcag tcaatcggag
    10441 gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc
    10501 gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca
    10561 ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga gggctggacc
    10621 gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt accacagata
    10681 tcctgtttgg cccatattca gctgttccat ctgttcttgg ccctgagccg gggcaggaac
    10741 tgcttaccac agatatcctg tttggcccat attcagctgt tccatctgtt cctgaccttg
    10801 atctgaactt ttctattctc agttatgtat ttttccatgc cttgcaaaat ggcgttactt
    10861 aagctagctt gccaaaccta caggtggggt ctttcacatg tatatgtcaa aaataaaaat
    10921 caactaattg actagtaatt aatatgactg gcataatggg aaattgatcc tgacagatgc
    10981 aaactggctt ctcagcagcg catttatgtt gtcaactgag gaaggaaacg ttaatgacag
    11041 aaactctaag taatttccac gtttatctat ttttatttat actagctttg gtaacaggaa
    11101 tattgcagca ttcatgcaca ttgaaaccct tatgaaataa aaacatctgt gcatttaaaa
    11161 tggaattaac attttaaatg ttaaaaaaag ctggcttagc ttccccccgc cccctagggc
    11221 atagaacaag tcaaatgctt tatatatttg agtttgggat gtattaggaa actcctaaga
    11281 gcaaagctag cttg
//
