LOCUS       sequence-237570-         596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-101-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-101-5p
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(94..118)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(342..361)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    407..462
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(461..480)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aagagacata cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
       61 aactggggca caaacttaat tgagaattct accacccagc tttcttgtac aaagtggtac
      121 gtctcaagac cagaggtctc tgcttctagc ggcagcatca gcactgtgat aactgagcat
      181 cagcactgtg ataactgagc atcagcactg tgataactga gcatcagcac tgtgataact
      241 gcaaccagaa gagacctgtg cttcactcct caggtgcagg ctgcctatca gaaggtggtg
      301 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
      361 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
      421 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
      481 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
      541 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaa
//
