LOCUS       sequence-237576-        9152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing G3BP1-GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA
  TITLE     Single-Molecule Imaging of mRNA Localization and Regulation during 
            the Integrated Stress Response.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 
            10.1016/j.molcel.2018.12.006.
  PUBMED    30661979
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 
            10.1016/j.molcel.2018.12.006."
COMMENT     Sequence Label: phage UbiC G3BP1-GFP-GFP
FEATURES             Location/Qualifiers
     source          1..9152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2625..2634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4047..4049,4050..4052,4053..4763)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4047..4049,4050..4052,4053..4763)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4809..4811,4812..4814,4815..5528)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4809..4811,4812..4814,4815..5525)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5542..6130
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5595..5615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6013..6024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6205..6438
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6557..6575)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6643..6747
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6748..6816,6817..7608)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6966..6985)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7779..8367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8268..8287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8521..8538
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8814..8819,8820..8837,8838..8844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8852..8868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8857..8879
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8876..8892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8876..8892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8942..8961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccgccacc atggtgatgg
     2641 agaagcctag tcccctgctg gtcgggcggg aatttgtgag acagtattac acactgctga
     2701 accaggcccc agacatgctg catagatttt atggaaagaa ctcttcttat gtccatgggg
     2761 gattggattc aaatggaaag ccagcagatg cagtctacgg acagaaagaa atccacagga
     2821 aagtgatgtc acaaaacttc accaactgcc acaccaagat tcgccatgtt gatgctcatg
     2881 ccacgctaaa tgatggtgtg gtagtccagg tgatggggct tctctctaac aacaaccagg
     2941 ctttgaggag attcatgcaa acgtttgtcc ttgctcctga ggggtctgtt gcaaataaat
     3001 tctatgttca caatgatatc ttcagatacc aagatgaggt ctttggtggg tttgtcactg
     3061 agcctcagga ggagtctgaa gaagaagtag aggaacctga agaaagacag caaacacctg
     3121 aggtggtacc tgatgattct ggaactttct atgatcaggc agttgtcagt aatgacatgg
     3181 aagaacattt agaggagcct gttgctgaac cagagcctga tcctgaacca gaaccagaac
     3241 aagaacctgt atctgaaatc caagaggaaa agcctgagcc agtattagaa gaaactgccc
     3301 ctgaggatgc tcagaagagt tcttctccag cacctgcaga catagctcag acagtacagg
     3361 aagacttgag gacattttct tgggcatctg tgaccagtaa gaatcttcca cccagtggag
     3421 ctgttccagt tactgggata ccacctcatg ttgttaaagt accagcttca cagccccgtc
     3481 cagagtctaa gcctgaatct cagattccac cacaaagacc tcagcgggat caaagagtgc
     3541 gagaacaacg aataaatatt cctccccaaa ggggacccag accaatccgt gaggctggtg
     3601 agcaaggtga cattgaaccc cgaagaatgg tgagacaccc tgacagtcac caactcttca
     3661 ttggcaacct gcctcatgaa gtggacaaat cagagcttaa agatttcttt caaagttatg
     3721 gaaacgtggt ggagttgcgc attaacagtg gtgggaaatt acccaatttt ggttttgttg
     3781 tgtttgatga ttctgagcct gttcagaaag tccttagcaa caggcccatc atgttcagag
     3841 gtgaggtccg tctgaatgtc gaagagaaga agactcgagc tgccagggaa ggcgaccgac
     3901 gagataatcg ccttcgggga cctggaggcc ctcgaggtgg gctgggtggt ggaatgagag
     3961 gccctccccg tggaggcatg gtgcagaaac caggatttgg agtgggaagg gggcttgcgc
     4021 cacggcaggg gggggggggg tctagaatgg tgagcaaggg cgaggagctg ttcaccgggg
     4081 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4141 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4201 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4261 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4321 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4381 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4441 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4501 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4561 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4621 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4681 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4741 tcggcatgga cgagctgtac aagggcacag gcagcaccgg ttcaggttca tcaggcacag
     4801 ctagttcaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     4861 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4921 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4981 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     5041 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     5101 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     5161 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     5221 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     5281 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     5341 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     5401 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     5461 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     5521 acaagtaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     5581 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5641 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5701 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5761 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5821 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5881 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5941 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6001 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6061 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6121 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6181 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6241 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6301 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6361 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6421 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6481 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     6541 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6601 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6661 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6721 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6781 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6841 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6901 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6961 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     7021 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7081 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7141 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7201 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7261 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7321 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7381 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7441 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7501 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7561 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7621 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7681 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7741 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7801 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7861 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7921 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7981 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8041 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8101 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8161 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8221 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8281 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8341 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8401 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8461 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8521 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8581 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     8641 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8701 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     8761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8881 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     8941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     9001 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     9061 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9121 atttgttaaa tatgtactac aaacttagta gt
//
