LOCUS       sequence-237578-       15249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 15249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
FEATURES             Location/Qualifiers
     source          1..15249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(102..120)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(103..120)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(410..429)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(643..662)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     762..784
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(822..840)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        908..1012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1013..1081,1082..1873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1231..1250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2044..2632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2533..2552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2786..2803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2873..2893)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2878..3207
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3058..3193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4341..4406
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4536..4556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4981..5115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5018..5037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5072..5091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5283..5916
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5963..6088
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6585..6818
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7003..7047
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7332..7449
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7612..8019
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8073..8093
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8142..8151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8148..8174,8175..8177,8178..10664,10665..10667,
                     10668..12251)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8148..12251
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12255..12281
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12261..12287
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             12300..12320
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12327..12347
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12426..12482
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12498..12554
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12570..12626
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12642..12698
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12714..12770
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12786..12842
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12858..12914
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12930..12986
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13002..13058
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13074..13130
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13146..13202
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13218..13274
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13290..13346
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13362..13418
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13434..13490
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13506..13562
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13578..13634
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13650..13706
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13722..13778
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13794..13850
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13866..13922
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13938..13994
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             14082..14138
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             14178..14198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14208..14264
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             14277..14978
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             join(15093..15249,1..77)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
       61 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
      121 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
      181 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
      241 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
      301 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
      361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
      541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
      601 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
      661 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      721 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      781 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
      841 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      901 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      961 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1021 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1081 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1141 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1201 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1261 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1321 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1381 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1441 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1501 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1561 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1621 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1681 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1741 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1801 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1861 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1921 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1981 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2041 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2101 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2161 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2221 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2281 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2341 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2401 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2461 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2521 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2581 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2641 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2701 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2761 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     2821 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     2881 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2941 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3001 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3061 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3121 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3181 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     3241 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     3301 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     3361 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     3421 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     3481 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     3541 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     3601 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     3661 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     3721 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     3781 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     3841 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     3901 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     3961 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     4021 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     4081 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     4141 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     4201 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     4261 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     4321 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     4381 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     4441 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     4501 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     4561 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     4621 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     4681 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     4741 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     4801 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     4861 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4921 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4981 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5041 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5101 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     5161 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     5221 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     5281 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     5341 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     5401 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     5461 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     5521 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     5581 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     5641 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     5701 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     5761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5941 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6001 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6061 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6121 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6181 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6241 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6301 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6361 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6421 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6481 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6541 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6601 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6661 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6721 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6781 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6841 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6901 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6961 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7021 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7081 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7141 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7201 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7261 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta
     7321 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     7381 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     7441 caaaattttc gggtttatta cagggacagc agagatccag tttggatcga taagcttgat
     7501 atcgaattat atcgaattcc tgcagccccg ataaaataaa agattttatt tagtctccag
     7561 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca
     7621 ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt
     7681 acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg
     7741 gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag
     7801 atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag
     7861 gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt
     7921 ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca
     7981 ctcggcgcgc cagtcctccg acagactgag tcgcccgggg gggatctgga gctctcgaga
     8041 attctcacgc gtcaagtacg cgcgccacca tgcccaagaa gaagcgcaag gtgggacgcg
     8101 tctgcaggat atcaagcttg cggtaccgcg ggcccgggat cgccaccatg gacaagaagt
     8161 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     8221 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     8281 agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga
     8341 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     8401 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     8461 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     8521 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     8581 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     8641 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     8701 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     8761 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     8821 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     8881 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     8941 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     9001 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     9061 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     9121 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     9181 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg
     9241 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     9301 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     9361 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     9421 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     9481 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     9541 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     9601 gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     9661 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
     9721 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     9781 aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     9841 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     9901 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     9961 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
    10021 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
    10081 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
    10141 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
    10201 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
    10261 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
    10321 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
    10381 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
    10441 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
    10501 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
    10561 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
    10621 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
    10681 gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg
    10741 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc
    10801 agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga
    10861 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
    10921 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
    10981 agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
    11041 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
    11101 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
    11161 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
    11221 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
    11281 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
    11341 caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga
    11401 aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
    11461 tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg
    11521 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
    11581 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
    11641 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
    11701 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
    11761 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
    11821 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
    11881 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact
    11941 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
    12001 ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc
    12061 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
    12121 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
    12181 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
    12241 tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc
    12301 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggac ggcattggta
    12361 gtgggagcaa cggcagcagc ggatccaacg gtccgggtgg atctggaggt ggaggttctg
    12421 gaggagaaga acttttgagc aagaattatc atcttgagaa cgaagtggct cgtcttaaga
    12481 aaggttctgg cagtggagaa gaactgcttt caaagaatta ccacctggaa aatgaggtag
    12541 ctagactgaa aaaggggagc ggaagtgggg aggagttgct gagcaaaaat tatcatttgg
    12601 agaacgaagt agcacgacta aagaaagggt ccggatcggg tgaggagtta ctctcgaaaa
    12661 attatcatct cgaaaacgaa gtggctcggc taaaaaaggg cagtggttct ggagaagagc
    12721 tattatctaa aaactaccac ctcgaaaatg aggtggcacg cttaaaaaag ggaagtggca
    12781 gtggtgaaga gctactatcc aagaattatc atcttgagaa cgaggtagcg cgtttgaaga
    12841 agggttccgg ctcaggagag gaactgctct cgaagaacta tcatcttgaa aatgaggtcg
    12901 ctcgattaaa aaagggatcg ggcagtggtg aggaactact ttcaaagaat taccacctcg
    12961 aaaacgaagt agctcgatta aagaaaggtt cagggtcggg tgaagaatta ctgagtaaaa
    13021 attatcatct ggaaaatgag gtagcgagac taaaaaaggg gagtggttct ggcgaagagt
    13081 tgctatcgaa aaattatcat cttgagaacg aagttgctag gctcaaaaag ggctcaggct
    13141 caggcgagga gttgctctcg aaaaactacc acttggaaaa tgaggtcgcg aggttgaaaa
    13201 aggggagcgg gtcgggcgag gagttattga gcaaaaacta tcatttagag aacgaagtcg
    13261 cgcgcttaaa gaaaggctcg ggctcgggcg aagaactctt atcgaagaac taccacctcg
    13321 aaaatgaggt cgccaggttg aaaaagggca gtggcagcgg ggaggaactc ttgagcaaga
    13381 actaccactt ggagaatgag gtcgcgagat tgaagaaagg gtcggggagc ggcgaggaat
    13441 tgctcagcaa gaattatcat ttggagaacg aagtcgccag gctcaagaaa ggctcggggt
    13501 cgggggagga attgttgagt aaaaactacc acttggaaaa tgaagtcgcc aggctcaaaa
    13561 aagggagtgg gagcggcgaa gagttattga gcaaaaatta ccacttggag aacgaagtgg
    13621 caaggctcaa gaaagggagc ggcagcgggg aggagctctt atcgaagaac taccacttag
    13681 agaatgaagt cgcccgcttg aagaaaggct cggggagcgg ggaagagctc ttgagcaaga
    13741 actaccactt ggaaaatgag gtggcgcgct tgaagaaagg gagcgggagc ggggaagagt
    13801 tactatctaa gaattatcat ctcgagaacg aggtggctcg actaaagaag ggctccggca
    13861 gtggggagga actcctgtcg aagaactatc atcttgaaaa tgaggttgca agacttaaaa
    13921 aggggtccgg atcaggtgag gaactactca gtaagaatta ccacctggaa aacgaagttg
    13981 cacgtttgaa gaaaggatca ggatcaggcg aagaactgct ctcaaaagat tatcatttgg
    14041 aaaatgaggt tgcacgttta aaaaagggaa gtggcagtgg tgaggaactt ctgtcgaaaa
    14101 attatcatct cgagaatgaa gtagcccgac ttaaaaaggg ttctggctcg ggtcagcggc
    14161 cgcaaggtgg aggtggaccc aagaagaagc gcaaggtggg aagcggagct actaacttca
    14221 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctgcacca ggaagtatga
    14281 gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
    14341 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
    14401 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
    14461 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
    14521 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
    14581 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
    14641 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
    14701 ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
    14761 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
    14821 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
    14881 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
    14941 gcgacctccc tagcaaactg gggcacaagc ttaattaagc ggccgcgact ctagagtcga
    15001 cctgcaggga tcgatcactg aggtaccttt aagaccaatg acttacaagg cagctgtaga
    15061 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
    15121 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
    15181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    15241 tcaagtagt
//
