LOCUS       sequence-237581-       10891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon 
            blue-light activation can bind up to 24 SspB-modified proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher 
            JE, Brangwynne CP
  TITLE     Mapping Local and Global Liquid Phase Behavior in Living Cells Using
            Photo-Oligomerizable Seeds.
  JOURNAL   Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 
            10.1016/j.cell.2018.10.048.
  PUBMED    30500534
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1467-1480.e13"
COMMENT     Sequence Label: M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1
FEATURES             Location/Qualifiers
     source          1..10891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..492
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      563..572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             638..1075
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             1106..1819
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1136..1164)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1759..1779
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    2520..3108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2573..2593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2991..3002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3111..3127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3112..3128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3218..3451
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3476..3494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3477..3494)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3784..3803)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4017..4036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4136..4158
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4196..4214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4282..4386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4387..4455,4456..5247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4605..4624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5418..6006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5907..5926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6160..6177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6247..6267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6252..6581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6432..6567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7715..7780
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7910..7930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8355..8489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8392..8411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8446..8465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8657..9290
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9337..9462
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9959..10192
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10377..10421
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10706..10823
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
       61 tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa
      121 ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa
      181 acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc
      241 gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc
      301 agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct
      361 gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt
      421 cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact
      481 gagtcgcccg ggggggatct ggagctctcg agaattctca cgcgtcaagt ggagcaaggc
      541 aggtggacag tggatcacta gtgccaccat ggcggagccg cgtagcaaac gtccgcgtgt
      601 gacgctgccg gcgtgtccgg cgcatagtgg ggagtttctg gcaaccacac tggaacggat
      661 cgagaaaaat ttcgtgatta ctgatccgag actgcctgac aacccaatca tttttgcgag
      721 cgattccttc ctgcagctga cagaatattc tcgggaagag atcctggggc gcaattgccg
      781 ttttctgcag ggacccgaga cagaccgtgc cactgttcgg aaaatcagag atgctattga
      841 caaccagact gaagtgaccg ttcagctgat caattatacc aagagcggca agaagttctg
      901 gaacgtgttc cacctgcagc cgatgcgcga ttataagggc gacgtccagt acttcattgg
      961 cgtgcagctg gatggcaccg aacgtcttca tggcgccgct gagcgtgagg cggtctgcct
     1021 gatcaaaaag acagcctttc agattgctga ggcagcgaac gacgaaaatt actttggcgg
     1081 ctccggctct ggatccggag ctaccatgag taaaggagaa gaacttttca ctggagttgt
     1141 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     1201 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     1261 actacctgtt ccatggccaa cacttgtcac taccctgact tatggtgttc aatgcttttc
     1321 aagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     1381 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
     1441 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     1501 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
     1561 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
     1621 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     1681 tgtcctttta ccagacaacc attacctgtc cacacaatct aagctttcga aagatcccaa
     1741 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     1801 catggatgaa ctatacaaag gtggatctgg aggttcaggt ggaagtgcgg ccggtaccgg
     1861 cagcggagga atgacgaccg cgtccacctc gcaggtgcgc cagaactacc accaggactc
     1921 agaggccgcc atcaaccgcc agatcaacct ggagctctac gcctcctacg tttacctgtc
     1981 catgtcttac tactttgacc gcgatgatgt ggctttgaag aactttgcca aatactttct
     2041 tcaccaatct catgaggaga gggaacatgc tgagaaactg atgaagctgc agaaccaacg
     2101 aggtggccga atcttccttc aggatatcaa gaaaccagac tgtgatgact gggagagcgg
     2161 gctgaatgca atggagtgtg cattacattt ggaaaaaaat gtgaatcagt cactactgga
     2221 actgcacaaa ctggccactg acaaaaatga cccccatttg tgtgacttca ttgagacaca
     2281 ttacctgaat gagcaggtga aagccatcaa agaattgggt gaccacgtga ccaacttgcg
     2341 caagatggga gcgcccgaat ctggcttggc ggaatatctc tttgacaagc acaccctggg
     2401 agacagtgat aatgaaagct gagcggccgc aactcccacc tgcaacatgc gtgactgact
     2461 gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     2521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2881 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2941 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3121 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     3181 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3241 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3421 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     3481 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     3541 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     3601 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     3661 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     3721 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     3781 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     3841 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     3901 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     3961 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     4021 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4081 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4141 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4201 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4261 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4321 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4381 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4441 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4501 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4561 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4621 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4681 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4741 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4801 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4861 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4921 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4981 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5041 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5101 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5161 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5221 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5281 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5341 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5461 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5521 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5581 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5641 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5701 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5761 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5821 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5881 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5941 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6001 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6061 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6181 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6241 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6301 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     6601 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     6661 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     6721 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     6781 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     6841 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     6901 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     6961 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     7021 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     7081 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     7141 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     7201 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     7261 cgggcgttgt tctttttaac ttcaggcgtg ttacaatagt ttccagtaag tattctggag
     7321 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     7381 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     7441 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     7501 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     7561 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     7621 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     7681 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     7741 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     7801 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     7861 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     7921 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     7981 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8041 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     8101 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     8161 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     8221 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     8281 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     8341 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8461 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     8521 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     8581 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     8641 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     8701 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     8761 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     8821 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     8881 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     8941 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     9001 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     9061 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     9121 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9181 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9241 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9301 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9361 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     9421 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     9481 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9541 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     9601 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     9661 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     9721 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     9781 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     9841 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9901 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9961 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    10021 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    10081 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    10141 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    10201 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    10261 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    10321 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    10381 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    10441 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    10501 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    10561 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    10621 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
    10681 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10741 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10801 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
    10861 tcgataagct tgatatcgaa ttcctgcagc c
//
