LOCUS       sequence-23760-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Shaikh AS, Tang YJ, Mukhopadhyay A, Keasling JD
  TITLE     Isotopomer distributions in amino acids from a highly expressed 
            protein as a proxy for those from total protein.
  JOURNAL   Anal Chem. 2008 Feb 1. 80(3):886-90.
  PUBMED    18179240
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal Chem. 
            2008 Feb 1. 80(3):886-90."
COMMENT     Sequence Label: pET30-R5-GFP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             104..148
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             164..178
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(278..306)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcgtaattcc ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtc tggtgccacg cggttctggt atgaaagaaa ccgctgctgc
      121 taaattcgaa cgccagcaca tggacagccc agatctgggt accgacgacg acgacaaggc
      181 catggcttct tcctctaaaa agtctggttc ctactctggt agcaaaggct ccaaacgtcg
      241 catcctggcc agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      301 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      361 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      421 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
      481 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
      541 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
      601 ccttgttaat cgtatcgagt taaaaggtat tgattttnaa gaagatggaa acattctcgg
      661 acacnaaact cgagtacnac tataactcac acaatgtata catcacggca gacaaacaaa
      721 agaatggaat caaagctaac tncaaattcg ccacancatt gaagatggat ccgttcaact
      781 agcagacntt atcacaaa
//
