LOCUS       sequence-237611-        3390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter paf from Penicillium chrysogenum domesticated into pUPD2 
            with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid 
            modular DNA assembly for A. tumefaciens-mediated genetic 
            transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB029
FEATURES             Location/Qualifiers
     source          1..3390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     409..426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(450..507)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1849..1870
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1873..1916
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1920..1938)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2100..2759
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2166..2185)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2780..2874
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(3057..3390,1..255)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
       61 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      241 cggagcctgt ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      421 agcgaggaag cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt
      481 ggtgacacct tgcccttttt tgccggactg cagcggccgc tactagtcgc gatgagtggt
      541 ctcacattta tgaagggctt gagatgatga tcctctggag aaggttaaag tggttgaaga
      601 tatcgaaatg ctgtgatggg agtgatacta tatggaattg agggtcatga agccgccttt
      661 ataaaccaga tcatgagctc tgtgtgaaag gtgacacata caatgctcag gtccaatgca
      721 gtcatcttgg gggtggcagc ttatggattg catgacttct ccaatgacat taatgcctca
      781 catacttcag gatactggac ctcttggctg gtatattctt acgggtgggg actctccaaa
      841 atcccttacc tgcttcagcc tccggggtaa gatgcactac ttgtttacca ggaagatatt
      901 tttgggctta cctagtggta atcatttcct aaaaagcaga gaaaacggct gacacgttga
      961 cacggtgact gggccactcg gatctgcaaa ggacgatggc tgcattgcat ttgataaagt
     1021 tgtttcacta ggaaatgatc tccaaatcca aaatgttggt atatgagaag gtttagcata
     1081 tggcggatgc ttataggtcg aaaaggcaaa ggcactatac tatgtatgta tctatgtgat
     1141 actgtgatac agccaagctg taaggcagct cccggttgtg ttacgtaggg ctggggtgga
     1201 ttcgagccaa agcaaattag gaaatgattc gcgggtagta tttcagtaac ataagctcac
     1261 atggtctagg cggtcaaaag ttgcatactt caaggtggga tgggtgggct tacagcggga
     1321 tgctagccct tgaacagggg ttgtcctcag tggcagcccc gcttctcctt ttgtgtaaca
     1381 aagtacatag tccgatcata ctgccaaggt tcgctcggcc gaaagagcag acagtaagcg
     1441 ctggaagagt aaatccaaat cttttaagat atacaggctt tcctacggtt gagcttaaaa
     1501 catgaagtat gaggggagtg gaaaaggtac tttcagtcag agaagagtat tgttagtggt
     1561 aaagaaattt attagctttc agatagagta gctaactgat ttaatatcgc actgatgaat
     1621 gaacactacg gtcattctcg aaatcggctt gaccattacg gagtacacac cactgaacaa
     1681 cccttttctg gccccatcgc caaggcctta gtgcagatca attattgagc aggaccgcat
     1741 taaattgggg ttgcagcaaa atgtgctttg catgctacca taaaatgcta cgtacattac
     1801 gcacttcgta cgaaaagctc tctgaattcc tcccgagacc actcatcgct ctagaagcgg
     1861 ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg
     1921 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1981 aggtggcact cgagttgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     2041 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     2101 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2161 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2221 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2281 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
     2341 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2401 cgttttcatc actctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2461 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2521 atatgttttt cgtatcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2581 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2641 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2701 tcggcagaat gcttaatgaa ttacaacagt actgcgacga gtggcagggc ggggcgtaat
     2761 ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
     2821 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     2881 ctcgagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2941 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3001 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3061 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3361 cagcggtcgg gctgaacggg gggttcgtgc
//
