LOCUS       sequence-237614-        8257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protocadherin gammaC5 expression (Myc-tag is at the linker between 
            EC1 and EC2 extracellular cadherin repeats).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali
            R, Chen WV, Maniatis T, De Blas AL
  TITLE     Molecular and functional interaction between protocadherin-gammaC5 
            and GABAA receptors.
  JOURNAL   J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 
            10.1523/JNEUROSCI.0969-12.2012.
  PUBMED    22915120
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0969-12"; journalName: "J Neurosci"; date: 
            "2012-08-22- 22"; volume: "32"; issue: "34"; pages: "11780-97"
COMMENT     Sequence Label: Myc-PCDHGC5
FEATURES             Location/Qualifiers
     source          1..8257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1072..1101
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3614..3631)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3620..3844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3890..4318
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3977..3996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4187..4208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4327..4347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4332..4661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4512..4647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4574..4593
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4728..5522
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4782..4801)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5392..5411
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5696..5817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5733..5752)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5787..5806
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5866..5882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5866..5882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5879..5901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5890..5906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5914..5920,5921..5938,5939..5944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5959..5980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6097..6114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6268..6856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6348..6367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7027..7818,7819..7887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7650..7669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7888..7992)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8067..8086)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagctaga ccgccatggg gcctatggca tcaccacagg tcactggtaa acggcaagtg
      721 ctgtatatgt tgtccttgtg ctgctgtggc tgggtgtctg ggcagcttcg ttattcagtg
      781 gtggaggaat cagagccggg gactttggtg gggaacgttg ctcaagatct gggcttaaag
      841 gtggcagatc tgttgagtcg caggctgaga ctgggctctg aggagaacgg gcgctatttt
      901 tccctgagct tgatgagtgg agctttggca gtgaatctaa aaattgaccg agagagcctg
      961 tgtggagcca gcacgagctg cctgctgcct gtacaggtgg taaccgaaca ccccctggag
     1021 ctcacccggg tagaggtaga gatcctagat ctcaacgaca actctcctag cgaacaaaaa
     1081 ctaatatcag aagaagacct atttgccact cctgagcgag agatgcgcat ctcagaatca
     1141 gcggcacctg gagccaggtt cccactggac agtgcccaag acccagacgt gggcaccaat
     1201 actgtgagct tttatactct aagccccagc agtcacttct ctctgaatgt gaagacccta
     1261 aaagatggga agctcttccc agagctagtg ctagagcagc agctagaccg ggaagcccag
     1321 gcaaggcatc agctggtact cactgctgtg gatgggggaa ccccagcccg ctcagggacc
     1381 agccttatct ctgtcattgt gctggacatc aatgataatg ccccaacctt ccagtcctca
     1441 gttctacgtg tgggactccc agagaacaca cccccgggga ccctgctgct ccgtctcaat
     1501 gccactgatc cagacgaggg caccaatggc caactagact actctttcgg agaccataca
     1561 tctgaagcag tgaagaatct ctttggcctg gatcctaaca ctggagcaat ccacgtattg
     1621 ggtccggtag attttgagga atcaaatttc tatgaaattc atgcaagagc tcgagaccag
     1681 ggacagccag ccatggaggg ccactgtgta atccaggtgg atgtagggga tgctaacgac
     1741 aatccccctg aggtgctgct ggcatctttg gtcaaccctg tcctagagag cacacccgtg
     1801 ggcacagttg tggggttgtt taatgtgcgg gaccgagact caggtagaaa tggtgaggtg
     1861 atcctggaga caactccaaa cctgccattt cagatcaagc cttccgagaa ccattactca
     1921 ctgctcacca gccagccttt ggaccgagag gtcacatccc attacaccat cgacctgctg
     1981 gccagtgatg ctggatcacc tcccctgcat acacatctca ccatcaggct gaacatttca
     2041 gatgttaatg acaacgcacc ccatttcacc caacagctct acactgctta tatcccagaa
     2101 aaccggccac caggctctct cctctgtact gtggctgcct ctgatccaga tgagggggat
     2161 aatgcccgcc tcacctattc tattgtggga agtcaaattc agggagcccc agcctcctcc
     2221 ttcgtgtatg tcaaccccga agatggacga gtctttgccc agcggacctt tgactatgaa
     2281 ttactgcaga tgctgcagat tgtggtgggg gtccgagatt ccggctctcc ccgactgtat
     2341 gctaacacat ctctccatgt gtttgtcctt gatcagaacg ataacgcccc aactgtactg
     2401 cacccacgat ctggcaggga gttctcagcc cctcagcgtc tcccccgatc tgctcctcct
     2461 ggttctctgg tcaccaaggt gacagctgtg gatgctgatg ctggtcacaa tgcatggctc
     2521 tcttactctc tattacctca gtccacagcc cctggactgt tcctcgtatc tacacacact
     2581 ggtgaggtgc gcacagcccg ggccttattg gaggacgact ctgataccca acaggtggtg
     2641 gtcctggtga gggacaatgg tgacccttca ctatcctcca cagcaacagt attgctggtt
     2701 ctggaggacg aggatgcaga ggaaatgccc aaatccagcg acttcctcac acaccctccc
     2761 gagcggtcag acctcaccct ttatcttatt gtggcgctgg cagctgtcag tctcttatcc
     2821 ctagttacct tcactttcat gtcagctaag tgtctgcgga ggcatgaaga tagggaccgg
     2881 ggtgggggcc agtgctgcag gggccaggac tcgccctcca gggagttcta taaacagtcc
     2941 agtcccaacc ttcaggtcag ctcagatggc actctaaagt acatggaggt gaccctgcgg
     3001 cccacagatt ctcagagcca ttgctacagg acgtgctttt ctccagcctc cgatggcagt
     3061 gacttcactt tcctcaggcc cctcagcgtt cagcagccct cagccttggc gctggagccc
     3121 gaggccttgc gttcccggtc tagtacgctg cgggagcgga gccagcaagc cccgcccaac
     3181 actgactggc gtttctctca agcccagaga cccggcacga gcggatccca aaatggggat
     3241 gaaaccggca cctggcccaa caaccagttc gatacagaga tgctgcaagc catgatcttg
     3301 gcctctgcca gtgaagccgc tgatgggagc tccactctgg gagggggcac tggcactatg
     3361 ggtttgagtg ctcgatatgg accccagttt accctgcagc acgtgcctga ctaccgccag
     3421 aacgtgtaca tccctggcag caatgccaca ctgaccaacg cagctggcaa acgagatggc
     3481 aaggctccgg caggcggcaa tggcaacaag aagaagtcgg gcaagaaaga gaagaagtaa
     3541 ccggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     3601 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3661 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3721 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3781 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3841 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     3901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3961 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4081 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4201 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4261 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4321 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4381 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4501 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4561 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4621 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4681 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4741 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4801 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4861 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     4921 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4981 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5041 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5101 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5161 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5221 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5281 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5341 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5401 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5461 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5521 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5581 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5641 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5701 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5761 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5821 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     5881 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5941 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6001 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6061 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6241 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7141 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7741 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     8041 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8101 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     8161 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     8221 gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
