LOCUS       sequence-237616-         512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-1249-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-1249-3p
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(108..132)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(356..375)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    421..476
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(475..494)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 caaggaggcc gacaaagaga catacgtcga gcagcacgag gtggctgtgg ccagatactg
       61 cgacctccct agcaaactgg ggcacaaact taattgagaa ttctaccacc cagctttctt
      121 gtacaaagtg gtacgtctca agaccagagg tctctgcttc gtacctgtga agaagggggg
      181 gaagggcgtt gaagaagggg gggaagggcg ttgaagaagg gggggaaggg cgttgaagaa
      241 gggggggaag ggcgtcaacc agaagagacc tgtgcttcac tcctcaggtg caggctgcct
      301 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
      361 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
      421 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
      481 gaaggacata tgggagggca aatcatttaa aa
//
