LOCUS       sequence-237617-         634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-1249-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-1249-5p
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(99..123)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(355..374)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    420..475
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(474..493)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
       61 tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct tgtacaaagt
      121 ggtacgtctc aagaccagag gtctctgctt cctcgtacaa cttggcccat ctcctccctc
      181 ctaacttggc ccatctcctc cctcctaact tggcccatct cctccctcct aacttggccc
      241 atctcctccc tcctcaacca gaagagacct gtgcttcact cctcaggtgc aggctgccta
      301 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
      361 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
      421 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
      481 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
      541 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
      601 gtatatgaaa cagccccctg ctgtccattc ctta
//
