LOCUS       sequence-237619-         597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-124a-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-124a-3p
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(97..121)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(337..356)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    402..457
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(456..475)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 acaaagagac atacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
       61 gcaaactggg gcacaaactt aattgagaat tctaccaccc agctttcttg tacaaagtgg
      121 tacgtctcaa gaccagaggt ctctgcttga cgagcgggca ttcaccgcgt gccttaggca
      181 ttcaccgcgt gccttaggca ttcaccgcgt gccttaggca ttcaccgcgt gccttacaac
      241 cagaagagac ctgtgcttca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
      301 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
      361 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
      421 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
      481 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
      541 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagcc
//
