LOCUS       sequence-237627-         631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-125b-2-3p target in 3' UTR of of mKate. miRNA activity can 
            be measured by the repression of mKate2 relative to EBFP2 
            fluorescent proteins . Plasmid is inherently unstable; please see 
            Depositor Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-125b-2-3p
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(99..123)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(347..366)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    412..467
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(466..485)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
       61 tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct tgtacaaagt
      121 ggtacgtctc aagaccagag gtctctgctt cggcaatagt cccaagagcc tgacttgtga
      181 gtcccaagag cctgacttgt gagtcccaag agcctgactt gtgagtccca agagcctgac
      241 ttgtgacaac cagaagagac ctgtgcttca ctcctcaggt gcaggctgcc tatcagaagg
      301 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
      361 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
      421 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
      481 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
      541 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
      601 aacagccccc tgctgtccat tccttattcc a
//
