LOCUS       sequence-237638-         573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-1271-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-1271-3p
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(93..117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(341..360)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    406..461
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(460..479)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 agagacatac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
       61 actggggcac aaacttaatt gagaattcta ccacccagct ttcttgtaca aagtggtacg
      121 tctcaagacc agaggtctct gcttatcctg gatgcctggc acatagcagg cacttgcctg
      181 gcacatagca ggcacttgcc tggcacatag caggcacttg cctggcacat agcaggcact
      241 caaccagaag agacctgtgc ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
      301 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
      361 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
      421 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
      481 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
      541 atatgctggc tgccatgaac aaaggttggc tat
//
