LOCUS       sequence-237639-        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-303
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3434..3523
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3536..3625
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4234..4252)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4248..4295
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4332..4787
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4419..4438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4629..4650
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4879..5694)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4879..5694)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5602..5621
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5686..5705
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5816..6404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6305..6324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6558..6575
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6590..6729
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6655..6674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6815..6837
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6831..7022)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7831..8913)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8875..8894
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8914..8991)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9197..9216
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9300..9319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9300..9318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9319..9343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9397..9414
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctccggatc agcagaggct tattttcgcc ggcaagcagc tagaggatgg ccgtacgttg
       61 gctgattaca atatccagaa ggaatccacc ctccacttgg tcctcaggct ccgtggtggt
      121 taagcttgca attaacttta agaaggagat atacaatgct tcacaagcga gctagtgaag
      181 ctaacgataa gaacgataat accatcatcg gttccgattt agcatcatct aagaaacgcc
      241 gtatcgattt cactgaatct tcgtctgata agtcatcttc gattttagct tctggtagta
      301 gcaggggttt tcacggcgat agcgtcgttc agcaaatcga catggctttt ggtaattcga
      361 accgtcagga gattgatgaa gatctgcaca gtcgccagct cgccgtctat ggtcgtgaga
      421 ctatgaggcg tctctttgct tccaatgttc tcatctcggg gatgcacggt cttggtgccg
      481 agattgccaa gaatcttata cttgctggtg tgaagtctgt gaccttgcat gatgaaagag
      541 ttgtagagct atgggactta tcaagcaact ttgttttctc ggaggatgat gttggcaaga
      601 atagagcaga tgcttctgtt cagaagttac aggatcttaa caatgctgtg gttgtctcta
      661 gcttgaccaa aagcttaaac aaagaggatc tttctggctt ccaggttgtt gtattctctg
      721 acataagcat ggaaagagca atcgaatttg atgactattg tcacagccat cagcctccta
      781 tcgcttttgt caaggctgat gtcaggggtc ttttcggctc tgtcttttgt gattttgggc
      841 ctgaatttgc ggttcttgat gttgatggag aggaacccca tacaggcatc attgcgtcta
      901 tctctaatga gaaccaggca tttatttcct gtgtcgacga tgagaggctt gagtttgaag
      961 atggtgacct agttgttttc tcagaagttg agggtatgac agaattgaat gatgggaaac
     1021 caaggaagat aaaaagtaca cggccatatt cattcaccct tgatgaggac actactaact
     1081 atggaacata tgtgaagggt ggtattgtca ctcaagtgaa acagccaaag ttactgaatt
     1141 tcaagccatt gagggaagcg cttaaggatc caggggattt tctatttagt gatttctcta
     1201 agtttgatcg gcctccactc cttcacttag cattccaggc acttgatcat tttaaagctg
     1261 aagctggcag atttcctgtt gctggctcag aagaggacgc tcagaagctt atatcgattg
     1321 ccacagccat caataccggg cagggtgatt taaaggtgga gaatgttgat cagaagcttc
     1381 taagacactt ttcctttgga gccaaggctg tcctgaatcc tatggctgca atgtttggcg
     1441 gtattgttgg acaggaggtt gttaaagctt gctctggaaa atttcatcct ctcttccagt
     1501 ttttctactt tgattcagtg gagtcactcc cctctgaacc tgtggactcg agtgactttg
     1561 caccaagaaa cagccggtat gatgcccaaa tatctgtatt tggggccaag ttccagaaga
     1621 aacttgaaga tgctaaagtt ttcacagtag ggtctggtgc tcttggctgt gagttcttga
     1681 aaaatttggc tctgatgggg gtttcttgtg gaagccaagg gaagctgaca gtgacagatg
     1741 atgatatcat tgagaagagt aacctcagtc gtcaatttct gttccgtgac tggaacattg
     1801 gacaggctaa atccacagtt gctgcttcgg ctgctgctgt tataaatcct cgcttcaaca
     1861 ttgaggccct gcaaaaccgt gttggcgcgg agactgagaa tgtatttgac gatgccttct
     1921 gggagaactt aactgttgtc gtcaatgcac tggataatgt taatgcgagg ctctatgttg
     1981 attcgaggtg cttgtatttc cagaagcctc tccttgagtc tggaactctt ggtacaaagt
     2041 gcaacacaca gtcggttatc ccacatctaa ctgaaaatta cggtgcctca agggacccac
     2101 cagagaaaca ggcccccatg tgtacagtgc attccttccc gcataacatt gatcactgtt
     2161 taacttgggc tcgctctgag tttgagggtc tgctggagaa aactcccgct gaagtgaatg
     2221 catatctttc tagcccagtt gagtacacta actcaatgat gagtgctggt gatgctcagg
     2281 cgagggacac attggagagg attgttgagt gccttgaaaa ggagaagtgt gaaaccttcc
     2341 aagactgctt aacctgggca cgactgaggt ttgaggatta ctttgtgaac cgtgtcaagc
     2401 aattgatata cacatttcct gaagatgctg cgactagtac cggagctcca ttctggtctg
     2461 ctccaaaaag attccctcgt ccactccagt actcgtcgtc cgacccaagt ctccttaact
     2521 ttatcacagc gactgctatt ttacgagcag agacatttgg gatcccgata cctgagtgga
     2581 ctaagaaccc aaaggaagca gcagaagctg ttgacagagt gatagtccca gactttgagc
     2641 caaggcaaga tgcaaagatt gtgactgatg agaaagctac cactttaacc actgcttcgg
     2701 tggacgacgc tgcagtcatc gacgatctca ttgctaagat cgaccagtgt aggcataact
     2761 tgtcgccaga cttcaggatg aaacctattc agttcgaaaa ggatgatgat acaaactatc
     2821 acatggatgt gatagcagga ctagccaaca tgagggcgag aaattacagc atccctgaag
     2881 tggacaagct gaaagcaaag ttcatagcag ggagaatcat cccagccatt gcgacttcaa
     2941 cagccatggc tactgggctg gtctgcctcg agctgtacaa ggtccttgat ggaggacata
     3001 aagtggaagc ttacagaaac acatttgcca acctggcact tccactattc tccatggctg
     3061 aaccacttcc accaaaggtg gtgaagcacc gtgacatggc ttggacagtt tgggacagat
     3121 gggttctcaa gggaaaccca actcttcgtg aggtgttgca gtggctggag gacaaagggc
     3181 ttagcgcata cagcatctct tgtggaagct gtctactgtt caacagtatg ttcacaaggc
     3241 acaaggagag gatggacaag aaggttgtgg atctcgctcg ggatgttgcg aaagtcgagt
     3301 taccgcctta tcgcaaccat cttgatgtag tggtggcttg tgaggatgaa gatgacaatg
     3361 acgtcgatat tcctctcgtc tctatctact tcaggtgagc ttactattaa ctttaagaag
     3421 gagatatacc atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     3481 ggactatgca ggattgtatc catatgacgt tccagattac gccactagag ctgcttaccc
     3541 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggattgta
     3601 tccatatgac gttccagatt acgcaatggc ttctaagagg atttcaaggg aactgaggga
     3661 tatgcagaga catcctccag caaactgtag cgcaggtcct gtggctgagg aggacatatt
     3721 ccattggcaa gcaactatca tgggaccgca tgacagtccg tattccggag gcgtatttac
     3781 ggtttctatt gatttctctt cggattatcc tttcaagcca ccaaaggtga atttcaagac
     3841 taaggtgtac cacccaaaca tcgacagcaa aggaagcatt tgccttgaca tattgaaaga
     3901 gcaatggagt cctgctccta ccacatccaa ggttttgttg tcgatttgct cgctgctgac
     3961 tgacccgaac ccaaatgatc ctcttgtgcc ggagatagct catctctaca aggtagataa
     4021 atcaaaatac gagtcaactg cacaaaaatg gacacagaag tacgcgatgg gatgagcttt
     4081 tacgctttta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4141 tctgctcccg gcatccgctt acagacaagc tgtgaccggg atgagatccg gctgctaaca
     4201 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     4261 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     4321 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4381 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4621 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4681 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4741 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     4801 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4861 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     4921 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     4981 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     5041 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     5101 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     5161 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     5221 gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca
     5281 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     5341 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     5401 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     5461 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     5521 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     5581 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     5641 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     5701 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     5761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5941 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6481 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6541 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     6601 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     6661 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     6721 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6781 ccggcatccg cttacagaca agctgtgacc gactccggga gctgcatgtg tcagaggttt
     6841 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     6901 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     6961 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     7021 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     7081 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     7141 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     7201 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     7261 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     7321 acacggaaac ccaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     7381 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     7441 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     7501 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     7561 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     7621 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     7681 tgcatgataa agaacacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     7741 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     7801 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7861 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7921 cgccagggtg gtttttcttt tcaccagtga cacgggcaac agctgattgc ccttcaccgc
     7981 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     8041 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcctcggtat cgtcgtatcc
     8101 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     8161 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     8221 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     8281 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     8341 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     8401 cacgcccagt cgcgtaccgt cctcatggga gaaaataata ctgttgatgg gtgtctggtc
     8461 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     8521 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     8581 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     8641 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     8701 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     8761 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     8821 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     8881 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     8941 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     9001 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     9061 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     9121 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     9181 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     9241 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     9301 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     9361 gccttaactt taagaaggag atataccatg ggcagccacc accaccacca ccacagcgga
     9421 atgcagatct ttgttaagac tctcaccgga aagacaatca ccctagaggt ggaaagctcc
     9481 gacaccatcg acaacgttaa ggccaagatc caggataagg agggcatt
//
