LOCUS       sequence-237640-        7530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protocadherin gamma C5 expression (mCherry-tag at the C-terminus)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali
            R, Chen WV, Maniatis T, De Blas AL
  TITLE     Molecular and functional interaction between protocadherin-gammaC5 
            and GABAA receptors.
  JOURNAL   J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 
            10.1523/JNEUROSCI.0969-12.2012.
  PUBMED    22915120
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0969-12"; journalName: "J Neurosci"; date: 
            "2012-08-22- 22"; volume: "32"; issue: "34"; pages: "11780-97"
COMMENT     Sequence Label: PCDHGC5-mCherry
FEATURES             Location/Qualifiers
     source          1..7530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3117..3126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3123..3833
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3268..3286)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3536..3555
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3954..4075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3991..4010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4045..4064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4082..4537)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4219..4240)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4431..4450
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4564..4668
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4670..5027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4670..4861
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4693..4713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4878..5013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5062..5856
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5062..5856
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5116..5135)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5726..5745
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6044..6063)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6088..6135
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6464..7052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6953..6972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7227..7530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gccgccatgg
      241 ggcctatggc atcaccacag gtcactggta aacggcaagt gctgtatatg ttgtccttgt
      301 gctgctgtgg ctgggtgtct gggcagcttc gttattcagt ggtggaggaa tcagagccgg
      361 ggactttggt ggggaacgtt gctcaagatc tgggcttaaa ggtggcagat ctgttgagtc
      421 gcaggctgag actgggctct gaggagaacg ggcgctattt ttccctgagc ttgatgagtg
      481 gagctttggc agtgaatcta aaaattgacc gagagagcct gtgtggagcc agcacgagct
      541 gcctgctgcc tgtacaggtg gtaaccgaac accccctgga gctcacccgg gtagaggtag
      601 agatcctaga tctcaacgac aactctccta gctttgccac tcctgagcga gagatgcgca
      661 tctcagaatc agcggcacct ggagccaggt tcccactgga cagtgcccaa gacccagacg
      721 tgggcaccaa tactgtgagc ttttatactc taagccccag cagtcacttc tctctgaatg
      781 tgaagaccct aaaagatggg aagctcttcc cagagctagt gctagagcag cagctagacc
      841 gggaagccca ggcaaggcat cagctggtac tcactgctgt ggatggggga accccagccc
      901 gctcagggac cagccttatc tctgtcattg tgctggacat caatgataat gccccaacct
      961 tccagtcctc agttctacgt gtgggactcc cagagaacac acccccgggg accctgctgc
     1021 tccgtctcaa tgccactgat ccagacgagg gcaccaatgg ccaactagac tactctttcg
     1081 gagaccatac atctgaagca gtgaagaatc tctttggcct ggatcctaac actggagcaa
     1141 tccacgtatt gggtccggta gattttgagg aatcaaattt ctatgaaatt catgcaagag
     1201 ctcgagacca gggacagcca gccatggagg gccactgtgt aatccaggtg gatgtagggg
     1261 atgctaacga caatccccct gaggtgctgc tggcatcttt ggtcaaccct gtcctagaga
     1321 gcacacccgt gggcacagtt gtggggttgt ttaatgtgcg ggaccgagac tcaggtagaa
     1381 atggtgaggt gatcctggag acaactccaa acctgccatt tcagatcaag ccttccgaga
     1441 accattactc actgctcacc agccagcctt tggaccgaga ggtcacatcc cattacacca
     1501 tcgacctgct ggccagtgat gctggatcac ctcccctgca tacacatctc accatcaggc
     1561 tgaacatttc agatgttaat gacaacgcac cccatttcac ccaacagctc tacactgctt
     1621 atatcccaga aaaccggcca ccaggctctc tcctctgtac tgtggctgcc tctgatccag
     1681 atgaggggga taatgcccgc ctcacctatt ctattgtggg aagtcaaatt cagggagccc
     1741 cagcctcctc cttcgtgtat gtcaaccccg aagatggacg agtctttgcc cagcggacct
     1801 ttgactatga attactgcag atgctgcaga ttgtggtggg ggtccgagat tccggctctc
     1861 cccgactgta tgctaacaca tctctccatg tgtttgtcct tgatcagaac gataacgccc
     1921 caactgtact gcacccacga tctggcaggg agttctcagc ccctcagcgt ctcccccgat
     1981 ctgctcctcc tggttctctg gtcaccaagg tgacagctgt ggatgctgat gctggtcaca
     2041 atgcatggct ctcttactct ctattacctc agtccacagc ccctggactg ttcctcgtat
     2101 ctacacacac tggtgaggtg cgcacagccc gggccttatt ggaggacgac tctgataccc
     2161 aacaggtggt ggtcctggtg agggacaatg gtgacccttc actatcctcc acagcaacag
     2221 tattgctggt tctggaggac gaggatgcag aggaaatgcc caaatccagc gacttcctca
     2281 cacaccctcc cgagcggtca gacctcaccc tttatcttat tgtggcgctg gcagctgtca
     2341 gtctcttatc cctagttacc ttcactttca tgtcagctaa gtgtctgcgg aggcatgaag
     2401 atagggaccg gggtgggggc cagtgctgca ggggccagga ctcgccctcc agggagttct
     2461 ataaacagtc cagtcccaac cttcaggtca gctcagatgg cactctaaag tacatggagg
     2521 tgaccctgcg gcccacagat tctcagagcc attgctacag gacgtgcttt tctccagcct
     2581 ccgatggcag tgacttcact ttcctcaggc ccctcagcgt tcagcagccc tcagccttgg
     2641 cgctggagcc cgaggccttg cgttcccggt ctagtacgct gcgggagcgg agccagcaag
     2701 ccccgcccaa cactgactgg cgtttctctc aagcccagag acccggcacg agcggatccc
     2761 aaaatgggga tgaaaccggc acctggccca acaaccagtt cgatacagag atgctgcaag
     2821 ccatgatctt ggcctctgcc agtgaagccg ctgatgggag ctccactctg ggagggggca
     2881 ctggcactat gggtttgagt gctcgatatg gaccccagtt taccctgcag cacgtgcctg
     2941 actaccgcca gaacgtgtac atccctggca gcaatgccac actgaccaac gcagctggca
     3001 aacgagatgg caaggctccg gcaggcggca atggcaacaa gaagaagtcg ggcaagaaag
     3061 agaagaaggc cggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     3121 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     3181 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     3241 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     3301 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     3361 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     3421 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     3481 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     3541 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     3601 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     3661 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     3721 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3781 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tagcggccgc
     3841 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3901 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     4081 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4141 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4201 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4261 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4321 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4381 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4441 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4501 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     4561 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4621 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     4681 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4801 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     5041 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5101 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5161 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5221 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     5281 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5341 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5401 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5461 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5521 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5581 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5641 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5701 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5761 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5821 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5881 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5941 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6001 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     6061 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     6121 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     6181 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     6241 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     6301 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6361 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6601 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7081 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7141 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     7201 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     7261 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     7321 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     7381 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     7441 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     7501 atctacgtat tagtcatcgc tattaccatg
//
