LOCUS       sequence-237641-        6797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway adapted mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-4"; volume: "109(36)"; pages: 
            "14476-81. Epub 2012 Aug 20"
COMMENT     Sequence Label: pBABEpuro-gateway
FEATURES             Location/Qualifiers
     source          1..6797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             39..508
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        539..643
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(644..712,713..1504)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(862..881)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1664..2252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2153..2172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2406..2423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             2553..3022
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(3243..3842)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3326..3346)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3823..3842
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3852..4181)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3866..4001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4166..4186
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     protein_bind    4223..4347
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4388..4693)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4490..4509)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5035..5694)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5609..5628
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(5748..5754,5755..5772,5773..5778))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5803..5927)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5955..6371)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5971..5987)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6003..6025)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6572..6771
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc gctgacgggt
       61 agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc ggatgcaact
      121 gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg aggactggcg
      181 ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc gcgcgaacag
      241 aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt caggtccttg
      301 gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga ccgcatctgg
      361 ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
      421 ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga actgcttacc
      481 acagatatcc tgtttggccc atattcaggc tgcaggtggc acttttcggg gaaatgtgcg
      541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      661 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      721 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      781 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      841 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      901 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      961 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1021 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1081 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1141 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1201 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1261 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1321 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1381 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1441 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1501 gtaactgtca gaccaagttt actcatatat actttagatt gatttgcggc cgcaaaagga
     1561 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1621 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1681 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1741 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1801 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1861 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1921 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1981 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2041 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2101 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2161 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2221 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2281 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2341 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2401 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2461 ccgcgcgttg gccgattcat taatgcaact atggccattt aatgtaaata cttaagaaaa
     2521 aaaaccaaat taattttgat acatgctgca tgtgaaagac ccccgctgac gggtagtcaa
     2581 tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga
     2641 gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgccccga
     2701 gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga
     2761 gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac
     2821 cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca
     2881 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     2941 tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct taccacagat
     3001 atcctgtttg gcccatattc agctgttcca tctgttcctg accttgatct gaacttctct
     3061 attctcagtt atgtattttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca
     3121 aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat
     3181 tttatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     3241 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     3301 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     3361 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     3421 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     3481 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     3541 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     3601 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     3661 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     3721 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     3781 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     3841 atggtaagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     3901 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     3961 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     4021 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     4081 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     4141 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ggtcgaccac
     4201 tgtgctggcg aattcctaca tcaccacttt gtacaagaaa gctgaacgag aaacgtaaaa
     4261 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     4321 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
     4381 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
     4441 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
     4501 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggagactt
     4561 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
     4621 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa
     4681 ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt catttcaata
     4741 aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga
     4801 cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac atcatatatg
     4861 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat
     4921 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
     4981 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac gcgtttacgc
     5041 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     5101 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     5161 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     5221 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     5281 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     5341 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     5401 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     5461 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     5521 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     5581 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     5641 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     5701 ttccttagct cctgaaaatc tcgacggatc ctaactcaaa atccacacat tatacgagcc
     5761 ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gccatagtga ctggatatgt
     5821 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     5881 tttatatcat tttacgtttc tcgttcagct tttttgtaca aacttgtgat gtaccaccac
     5941 actgggatcc ggccggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc
     6001 ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga
     6061 ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa ggcttcccag
     6121 gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc
     6181 ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc gacgttaaag
     6241 gttggccatt ctgcagagca gaaggtaacc caacgtctct tcttgacatc taccgactgg
     6301 ttgtgagcga tccgctcgac atctttccag tgacctaagg tcaaacttaa gggagtggta
     6361 acagtctggc cctaattttc agacaaatac agaaacacag tcagacagag acaacacaga
     6421 acgatgctgc agcgctgcag cagacaagac gcgcggcttc ggttccaaac cgaaagcaaa
     6481 aattcagacg gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac cacatatcct
     6541 gacggggtcg gattccacat cgactccctt cctcaggtcg ggccacaaaa acggccccca
     6601 aagtccctgg gacgtctccc agggttgcgg ccgggtgttc agaactcgtc agttccacca
     6661 cgggtccgcc agatacagag ctagttagct aactagtacc gacgcaggcg cataaaatca
     6721 gtcatagaca ctagacaatc ggacagacac agataagttg ctggccagct tgcctcccgg
     6781 tggtgggtcg gtggtcc
//
