LOCUS       sequence-237643-        7541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protocadherin gamma C5 expression (EGFP-tag at the C-terminus)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali
            R, Chen WV, Maniatis T, De Blas AL
  TITLE     Molecular and functional interaction between protocadherin-gammaC5 
            and GABAA receptors.
  JOURNAL   J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 
            10.1523/JNEUROSCI.0969-12.2012.
  PUBMED    22915120
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0969-12"; journalName: "J Neurosci"; date: 
            "2012-08-22- 22"; volume: "32"; issue: "34"; pages: "11780-97"
COMMENT     Sequence Label: PCDHGC5-EGFP
FEATURES             Location/Qualifiers
     source          1..7541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3117..3126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3123..3125,3126..3128,3129..3842)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCLSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSAQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3168..3189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3429..3448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3776..3797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3965..4086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4002..4021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4056..4075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4093..4548)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4230..4251)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4442..4461
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4575..4679
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4681..5038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4681..4872
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4704..4724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4889..5024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5073..5867
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNVMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5127..5146)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5737..5756
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6055..6074)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6099..6146
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6475..7063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6964..6983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7238..7541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gccgccatgg
      241 ggcctatggc atcaccacag gtcactggta aacggcaagt gctgtatatg ttgtccttgt
      301 gctgctgtgg ctgggtgtct gggcagcttc gttattcagt ggtggaggaa tcagagccgg
      361 ggactttggt ggggaacgtt gctcaagatc tgggcttaaa ggtggcagat ctgttgagtc
      421 gcaggctgag actgggctct gaggagaacg ggcgctattt ttccctgagc ttgatgagtg
      481 gagctttggc agtgaatcta aaaattgacc gagagagcct gtgtggagcc agcacgagct
      541 gcctgctgcc tgtacaggtg gtaaccgaac accccctgga gctcacccgg gtagaggtag
      601 agatcctaga tctcaacgac aactctccta gctttgccac tcctgagcga gagatgcgca
      661 tctcagaatc agcggcacct ggagccaggt tcccactgga cagtgcccaa gacccagacg
      721 tgggcaccaa tactgtgagc ttttatactc taagccccag cagtcacttc tctctgaatg
      781 tgaagaccct aaaagatggg aagctcttcc cagagctagt gctagagcag cagctagacc
      841 gggaagccca ggcaaggcat cagctggtac tcactgctgt ggatggggga accccagccc
      901 gctcagggac cagccttatc tctgtcattg tgctggacat caatgataat gccccaacct
      961 tccagtcctc agttctacgt gtgggactcc cagagaacac acccccgggg accctgctgc
     1021 tccgtctcaa tgccactgat ccagacgagg gcaccaatgg ccaactagac tactctttcg
     1081 gagaccatac atctgaagca gtgaagaatc tctttggcct ggatcctaac actggagcaa
     1141 tccacgtatt gggtccggta gattttgagg aatcaaattt ctatgaaatt catgcaagag
     1201 ctcgagacca gggacagcca gccatggagg gccactgtgt aatccaggtg gatgtagggg
     1261 atgctaacga caatccccct gaggtgctgc tggcatcttt ggtcaaccct gtcctagaga
     1321 gcacacccgt gggcacagtt gtggggttgt ttaatgtgcg ggaccgagac tcaggtagaa
     1381 atggtgaggt gatcctggag acaactccaa acctgccatt tcagatcaag ccttccgaga
     1441 accattactc actgctcacc agccagcctt tggaccgaga ggtcacatcc cattacacca
     1501 tcgacctgct ggccagtgat gctggatcac ctcccctgca tacacatctc accatcaggc
     1561 tgaacatttc agatgttaat gacaacgcac cccatttcac ccaacagctc tacactgctt
     1621 atatcccaga aaaccggcca ccaggctctc tcctctgtac tgtggctgcc tctgatccag
     1681 atgaggggga taatgcccgc ctcacctatt ctattgtggg aagtcaaatt cagggagccc
     1741 cagcctcctc cttcgtgtat gtcaaccccg aagatggacg agtctttgcc cagcggacct
     1801 ttgactatga attactgcag atgctgcaga ttgtggtggg ggtccgagat tccggctctc
     1861 cccgactgta tgctaacaca tctctccatg tgtttgtcct tgatcagaac gataacgccc
     1921 caactgtact gcacccacga tctggcaggg agttctcagc ccctcagcgt ctcccccgat
     1981 ctgctcctcc tggttctctg gtcaccaagg tgacagctgt ggatgctgat gctggtcaca
     2041 atgcatggct ctcttactct ctattacctc agtccacagc ccctggactg ttcctcgtat
     2101 ctacacacac tggtgaggtg cgcacagccc gggccttatt ggaggacgac tctgataccc
     2161 aacaggtggt ggtcctggtg agggacaatg gtgacccttc actatcctcc acagcaacag
     2221 tattgctggt tctggaggac gaggatgcag aggaaatgcc caaatccagc gacttcctca
     2281 cacaccctcc cgagcggtca gacctcaccc tttatcttat tgtggcgctg gcagctgtca
     2341 gtctcttatc cctagttacc ttcactttca tgtcagctaa gtgtctgcgg aggcatgaag
     2401 atagggaccg gggtgggggc cagtgctgca ggggccagga ctcgccctcc agggagttct
     2461 ataaacagtc cagtcccaac cttcaggtca gctcagatgg cactctaaag tacatggagg
     2521 tgaccctgcg gcccacagat tctcagagcc attgctacag gacgtgcttt tctccagcct
     2581 ccgatggcag tgacttcact ttcctcaggc ccctcagcgt tcagcagccc tcagccttgg
     2641 cgctggagcc cgaggccttg cgttcccggt ctagtacgct gcgggagcgg agccagcaag
     2701 ccccgcccaa cactgactgg cgtttctctc aagcccagag acccggcacg agcggatccc
     2761 aaaatgggga tgaaaccggc acctggccca acaaccagtt cgatacagag atgctgcaag
     2821 ccatgatctt ggcctctgcc agtgaagccg ctgatgggag ctccactctg ggagggggca
     2881 ctggcactat gggtttgagt gctcgatatg gaccccagtt taccctgcag cacgtgcctg
     2941 actaccgcca gaacgtgtac atccctggca gcaatgccac actgaccaac gcagctggca
     3001 aacgagatgg caaggctccg gcaggcggca atggcaacaa gaagaagtcg ggcaagaaag
     3061 agaagaaggc cggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     3121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3301 ccctcgtgac caccctgacc tacggcgtgc agtgcctcag ccgctacccc gaccacatga
     3361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gcgcagctcg
     3661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3721 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     3781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     3841 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3901 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3961 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4081 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4141 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4201 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4261 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4321 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4381 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4441 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4501 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     4561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     4681 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4861 agccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4921 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg tcgcctcggc
     4981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     5041 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5101 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5161 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5221 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     5281 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcggggaggg
     5341 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5401 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5461 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5521 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5581 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5641 atgcctgctt gccgaatgtc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5701 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5761 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5821 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5881 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5941 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6001 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     6061 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     6121 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     6181 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     6241 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     6301 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     6361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6421 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6601 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccggtgg ctgctgccag
     6721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7141 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     7201 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     7261 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     7321 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     7381 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     7441 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     7501 cttggcagta catctacgta ttagtcatcg ctattaccat g
//
