LOCUS       sequence-237644-        5868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RING finger protein 34 expression (HA-tag at the N-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Jin H, Chiou TT, Serwanski DR, Miralles CP, Pinal N, De Blas AL
  TITLE     Ring finger protein 34 (RNF34) interacts with and promotes 
            gamma-aminobutyric acid type-A receptor degradation via 
            ubiquitination of the gamma2 subunit.
  JOURNAL   J Biol Chem. 2014 Oct 17;289(42):29420-36. doi: 
            10.1074/jbc.M114.603068. Epub 2014 Sep 5.
  PUBMED    25193658
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.603068"; journalName: "J Biol Chem"; date: 
            "2014-10-17- 17"; volume: "289"; issue: "42"; pages: "29420-36"
COMMENT     Sequence Label: HA-RNF34
FEATURES             Location/Qualifiers
     source          1..5868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          128..1144
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1068..1090
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1152..1171
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1202..1228
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2388..2407)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2453..2508
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2507..2526)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2869..2885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2869..2885)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2882..2904)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2893..2909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2917..2923,2924..2941,2942..2947))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2962..2983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3042..3237
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3088..3223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3243..3377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3280..3299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3334..3353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3445..3462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3616..4204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3696..3715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4375..5166,5167..5235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4998..5017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5236..5340)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5371..5750
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
       61 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      121 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      181 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      241 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      301 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      361 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      421 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
      481 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
      541 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
      601 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
      661 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
      721 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
      781 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
      841 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
      901 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
      961 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1021 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1081 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1141 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcat
     1201 gtacccatac gatgttccag attacgctgc tagcctcgag atgaaggcgg gtgctacctc
     1261 tatgtgggct tcatgctgtg gactgctgaa tgaagtcatg ggaactggag ctgtcagagg
     1321 ccagcaggca ggattcccag gaagcactgg tccattcagg tttacaccga gttctgactt
     1381 tcctacctac ccaccagcag ctacagaagg acccaatata gtttgcaaag cctgtggact
     1441 ttcattttca gtctttagaa agaagcacgt ctgctgtgac tgcaagaagg acttttgctc
     1501 cctttgttcg gtctcacaag aaaacctccg cagatgctct acatgtcacc tactccaaga
     1561 gacagccttc cagcgccctc agttgatgag actaaaagtg aaggacctgc ggcagtatct
     1621 gctcctcaga aacgtaccga ccgacacttg tcgcgagaag gaggacttag tggatctagt
     1681 gctgtgccat cgtggactgg gctcggggga tggcctggac tcaaggagtc tcagttcctc
     1741 aagatcccag acttctagtt tttttacaca atcctatttt tcaaattata cacccccctc
     1801 tgctactgtg tcctccttcc agggagagct gatggacaga gagggcacct tcaggtcaga
     1861 agttctcaca caggtacaaa gtgaattagc ttcagcaaac acggatgatg aggatgggga
     1921 ggaagatgac gacgatgatg acgacgacga cgacgaggat gacgatgaac aagaagaaaa
     1981 cttagaggaa cagaacctgg ggctctctaa gaagaaggcg agagcttccc tgtccgacct
     2041 gtcaagcctt gaagaggtgg aagggatgag cgtgcgccag ctgaaggaaa tcctggctcg
     2101 gaattttgtc aactattctg gctgctgtga aaagtgggag ctggtggaga aagtcaaccg
     2161 gctgtacaag gagaatgagg aaaaccaaaa gtcatatggt gagcggatgc aacttcagga
     2221 tgaggaagac gacagcctgt gccgaatctg catggatgcg gtcattgact gtgtcctgct
     2281 ggagtgtggg cacatggtca cctgcaccaa gtgcggcaag cgcatgagtg agtgtcccat
     2341 ctgcaggcag tatgtggttc gagctgtgca tgttttcaag tcctagatct ttttccctct
     2401 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     2461 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     2521 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     2581 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     2641 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     2701 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     2761 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     2821 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     2881 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2941 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3001 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     3061 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3121 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3181 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3241 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3361 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5341 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     5401 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     5461 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     5521 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5581 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     5641 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     5701 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     5761 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     5821 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggg
//
