LOCUS       sequence-237663-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00081821
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(405..421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(405..421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(418..440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    429..445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(453..459,460..477,478..483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    498..519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(636..653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcaatta atttcaacac ccaaagatct caaagtaacc aatggaatag
       61 catgcatatc agtaccagca taagtcaaat gtggtggcaa ttcatgacaa gttggtttat
      121 cacaatcatc tttaacagta catctatatt tttcaccaga tctaacagta ataaaaactt
      181 cagagttcaa agcagtatca gcttcaccca taactggtct atcttgattt ctaccagatg
      241 gcaaaccata aataataata ttacatcttg gtctagacaa agtagatcta gacaaagcaa
      301 tcatattagc tttttgttca gcagtccatg gttctggttt tctaccacca cccattagag
      361 accaagttcc atcccaatgg cgcgccgagc ttggctcgag catggtcata gctgtttcct
      421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggcc
//
