LOCUS       sequence-237672-        1458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pET28B-PU.1-ETS-S3
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             376..393
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             415..432
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(485..503)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      499..546
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      583..1038
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(670..689)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     880..901
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1331..1340
                     /estimated_length=10
     gap             1419..1428
                     /estimated_length=10
ORIGIN
        1 gnnnnntnnn cnctnnanta ntttngttna actttaagaa ggagatatac catgggttcc
       61 aaaaaaaaga ttcgtttata ccagtttttg cttgatttgc ttcgtagtgg agacatgaaa
      121 gattcgatct ggtgggtaga taaggacaaa ggcacttttc aattctctag caaacataaa
      181 gaggctttgg cacaccgttg ggggatccaa aaaggtaacc gcaagaagat gacgtatcaa
      241 aaaatggccc gcgccttacg caattacgga aaaacgggca ttatccacaa gacggctaag
      301 aaaaaactta cctatcaatt tagcggcgaa gttcttgggc gtggcgggtt agccgaacgc
      361 cgtcttccgc cgcatcttgt tccgcgcggc tcgaagcttg cggccgcact cgagcaccac
      421 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      481 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      541 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      601 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      661 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      721 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      781 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      841 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      901 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      961 cggcctattg gttaaaaant gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1021 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccntatttg
     1081 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaanct
     1141 catcgagcat caaatgaanc tgccatttat tcnnatcagg attatccata ccatattttt
     1201 gaaaangccg ttnnngttat gaanganaat actcaccgag gcagttccna anganggnag
     1261 antctggnnn cggnntgnna ttccgannnt ccaaantcaa ncaaccannn aattnctcnn
     1321 ncnannnnta nnnnnnnnnn gtananncnn nnnnngtacn nnngancngg nnaatangna
     1381 annnttnnnc nnattttnnn nnntngnnca gnnncancnn nnnnnnnncn tcaancnnnn
     1441 nnnnannnnn nnnntnat
//
