LOCUS       sequence-237685-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mtagRFP-T under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP611
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..600
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     primer_bind     complement(2133..2149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2133..2149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2156..2180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3608..3628)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3656..3852)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3858..4673)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3858..4673)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4581..4600
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4683..4705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4694..4710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4719..4724,4725..4740,4741..4746))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      5101..5689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5590..5609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5752..6915
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6938..6958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8294..8318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8464..8475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10415..10432
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10416..10432
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
       61 tgaatttttc ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata
      121 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
      181 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
      241 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
      301 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
      361 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
      421 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
      481 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
      541 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
      601 ctttgctcta gaatggtctc caagggagag gagcttatca aggagaacat gcacatgaag
      661 ctctacatgg agggaaccgt caacaaccac cacttcaagt gcacctccga gggagaggga
      721 aagccatacg agggaaccca aaccatgcgt atcaaggtcg tcgagggagg accactccca
      781 ttcgccttcg acatcctcgc cacctccttc atgtacggat cgcgtacctt catcaaccac
      841 acccaaggaa tcccagactt cttcaagcaa tccttcccag agggattcac ctgggagcgt
      901 gtcaccacct acgaggacgg aggagtcctc accgccaccc aagacacctc cctccaagac
      961 ggatgcctca tctacaacgt caagatccgt ggagtcaact tcccatccaa cggaccagtc
     1021 atgcaaaaga agaccctcgg atgggaggcc aacaccgaga tgctctaccc agccgacgga
     1081 ggacttgagg gacgtaccga catggccctc aagctcgtcg gaggaggaca cctcatctgc
     1141 aacttcaaga ccacctaccg ttccaagaag ccagccaaga acctcaagat gccaggagtc
     1201 tactacgttg accaccgtct tgagcgtatc aaggaggccg acaaggagac ctacgtcgag
     1261 caacacgagg tcgccgtcgc ccgttactgc gacctcccat ccaagctcgg acacaagctc
     1321 aacggaatgg acgagcttta caagtaaggc cgctgtcatc agacatctcg cgcccgtgcc
     1381 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     1441 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     1501 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     1561 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     1621 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     1681 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     1741 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     1801 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     1861 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     1921 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     1981 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     2041 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tgcccactag
     2101 tgagtcgtat tacgtagctt ggcgtaatca tggtcatagc tgtttcctgc ccgtgaccca
     2161 gctttcttgt acaaagtggt aagtgcaagt aagatcagtg tttgtttcga tatcagtctg
     2221 tttcgtaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc acaactgaga
     2281 aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag
     2341 tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg
     2401 tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc aattatttca
     2461 tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca tacaaacgag
     2521 gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct aattagctat
     2581 ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt ctaattagaa
     2641 atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga aatgatcccg
     2701 cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt acacaattca
     2761 agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa aaggaggaaa
     2821 gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag gtgagtaacc
     2881 atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt aacagtttcg
     2941 aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata gcctaaacga
     3001 gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt tatcctttca
     3061 aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt tatatcatat
     3121 ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg tagatcgaca
     3181 acaaggcctc cacttcttca tcgatggcct ctgcagcacc tgcaccacca agtttgtatt
     3241 tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg ccagatcgat
     3301 gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg gccacggatt
     3361 gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac aaaatatggt
     3421 tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag atattttggg
     3481 tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac acaaaaagtt
     3541 cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat cgccgtgttc
     3601 aaaaacacaa ctttattata catagttgat aattcgatgg tatcagctca ctcaaagtat
     3661 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
     3721 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
     3781 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
     3841 tttattgtca gtactgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     3901 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     3961 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     4021 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     4081 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     4141 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     4201 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     4261 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     4321 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     4381 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     4441 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     4501 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     4561 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     4621 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cattttatgt
     4681 ttcctgtgtg aaattgttat ccgctcacaa ttccacagat tatacgagcc gatgattaat
     4741 tgtcaacagc tctttatgtt cggatgtgat gtgagaactg tatcctagca agattttaaa
     4801 aggaagtata tgaaagaaga acctcagtgg caaatcctaa ccttttatat ttctctacag
     4861 gggcgcggcg tggggacaat tcaacgcgtc tgtgagggga gcgtttccct gctcgcaggt
     4921 ctgcagcgag gagccgtaat ttttgcttcg cgccgtgcgg ccatcaaaat gtatggatgc
     4981 aaatgattat acatggggat gtatgggcta aatgtacggg cgacagtcac atcatgcccc
     5041 tgagctgcgc acgtcaagac tgtcaaggag ggtattctgg gcctccatgt cgggatcttc
     5101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5221 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     5281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5701 cgcggccttt ttacggttcc tggccttttg ctggccgaaa agtgccacct gaacgaagca
     5761 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     5821 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     5881 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     5941 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     6001 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     6061 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     6121 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     6181 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     6241 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     6301 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     6361 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     6421 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     6481 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     6541 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     6601 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     6661 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     6721 cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     6781 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     6841 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     6901 ttatgcttaa atgcgcgcca gggatccgcc aagctatcaa ctttgtatag aaaagttgtg
     6961 tcggattatg ggctcttctt ttatcctcta ctttctttct agaaaatgca aatgttgagt
     7021 cagaaattgc tgaatggagt acaactgcaa tgggtttagc ttatgtttct ggaacaatta
     7081 ctggtggaat tgtgattgaa aagtaaggtt atatttcatt tcaataaatg ttaccacagt
     7141 ataacaaaaa caagttacat tgttcagagt atgtctgaat gtattattat tattgtattt
     7201 gtgcatcagt actgtagttg tactgaagaa gggtttaaaa taatctcaac gtttttaaga
     7261 cgagttagtg agaatacggt cgcagttagg atgtttggac atagttttat tagataatcg
     7321 tgaactaact cactataaaa acccgctaac tcattgaata agatagtttt aaacgttcaa
     7381 aatttgaaga caaaattgta tgaataagtt tacagagttg gtcgacgcaa gcttctcctt
     7441 ctcttcacat ttctcaataa tttagctcta attgcattcg tattcttcgc aaaaatacgt
     7501 attctgattg acccgatgaa atacggatgc ctagttgcat tgatcatcta cggttatact
     7561 tatgggtgag gtttttcaaa tatattttga tttaatcgaa taatgaaatt tcagaactgg
     7621 agttggtcca atttcgtggt ttatatcctc tgaactggta cctcaaaaac atcgtagtat
     7681 tgctcaatct gtggcatatt caatgaatac tgtaatggtg gttatcagta catttacagt
     7741 attacctcta tattccgtta ttggtagtta tgctttcctt attctatatt caattccttc
     7801 atttataagt atgcttatat tatttcgcta cttaccggaa accaaaggac gagagattca
     7861 tgatattgtg aatgaactta aaaaaaaatg aaaacctttg aaaacgttca tacggccgaa
     7921 attttacttg atcgagccaa ttgaactttt gttaagactt acagaaaagt gagagatttt
     7981 gttacaaaaa agtgagaaaa aagcgaaaat gaacaggaaa aagaagaaaa atacacccga
     8041 agtatcatat aaaaattaaa aagattgttt gacctggcgg aacttttttg caaaaactct
     8101 gaatatccat ggcacatgct tcgtgcaaaa caggatccaa atgaatatca acaagagatt
     8161 cttgaagacg acgagccact tgctcagagc attgcttatc tcgaatgaga cccttgttaa
     8221 aatcattttt caaacattat atgacattgt cgaaatgtcc tcctgattcc atgatggtag
     8281 caaactcact tcgtcaagtt tgtacaaaaa agcaggcttg agccaattta tccaagtcct
     8341 tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat caaaaacaat
     8401 ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg gctcaaagtt
     8461 agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc ccaacaaggg
     8521 atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca aattcatcgc
     8581 ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt ctgcacccta
     8641 tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt ctccccagct
     8701 ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct ctctgacgtc
     8761 ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt ttcccatttc
     8821 tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt ttttttctgt
     8881 cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc tttttcttct
     8941 tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac cccgcggttc
     9001 gcaataggtt tctttttcaa attattagcg ttataaatag aaaaatggtg gagtttcaat
     9061 aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt tgaaattttt
     9121 cataccagtt ttccggaaat ccatctgata tcattatcgt atttttcttt ttaaaaatgt
     9181 tttcaaaaaa aaacaaaata tagctggtta tttggcaccc tctaattacc attttcctgt
     9241 cacaccacac tcttttcctt cttccacttc tttccgtttt cagccgcttc caaccaaacc
     9301 gatatgaaag ccgagcaaca acgatcgatt ccacccggct cggcgacctt cccgtcgcag
     9361 gtgagactca gaaaactaga gaaaccgctc aactaactct tgatatccga tttcattctt
     9421 ttctcttttc tttttgttga acttttccat atttccagat gccacggcca ccaccaagca
     9481 ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa agctcaaatc actccgaacg
     9541 acgttttagc acttccggga atcactcaag gtatcttgct ccctttggat ttcagtttaa
     9601 aacataaatt taggattctt atgctcccca tctgcgaaca tctataacat cgagttcacc
     9661 aagttccaaa tccgtgatct ggacactgag caagtgcttt tcgagatcgc caaaccggag
     9721 aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg ttatagattt
     9781 gctccaaact ttttgaaact caaaacggtc ggagcaactg tggagttcaa agtaggagac
     9841 atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg ccttctgaag
     9901 tgttttgact ttgaatttgg attctgtatt ccgaactcac gaaacaactg tgaacatatc
     9961 tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa agtacaattc
    10021 aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga gactcgttct
    10081 gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga ctactcatat
    10141 gatgcataaa tatttaatac aaaaatgttc tggataatta ttctgtcgaa tagaaaaaaa
    10201 actccaaatg tgattaaatt ccaataattc ctgtctagtt tgttccttcc ccttcccctt
    10261 ctcatgttca atgcattcct aagcttttca gttcccccct tgtttctata ttttttcgct
    10321 gtcctgtcac actcgctaaa aacactaatc acacggaaat ctgttttcaa taaaaactcc
    10381 aactttaact cattttcaat ttcaactgca cgggtgtaaa acgacggcca gtccgtaata
    10441 cgactcactt aaggcc
//
