LOCUS       sequence-237686-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF E648Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-E648Q
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          221..1159
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1116..1136
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1188..1212
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1213..1222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3517..3541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3549..3567
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3592..3611)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3593..3611)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3617..3637)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3691..3732
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3753..4320
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3770..4322
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3914..3931)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4141..4160
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4334..4933
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4334..4353)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4830..4850
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4947..5535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5000..5020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5418..5429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5610..5843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5962..5980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6048..6152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6153..6221,6222..7013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6371..6390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7184..7772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7673..7692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7926..7943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8183..8204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8219..8224,8225..8242,8243..8249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8257..8273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8262..8284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8281..8297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8281..8297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8347..8366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8558..9191
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9238..9363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9860..10093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10278..10322
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10615..10732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10780..10790,1..1168)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
       61 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
      121 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
      181 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
      241 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
      301 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
      361 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
      421 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
      481 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
      541 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
      601 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
      661 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
      721 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
      781 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
      841 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
      901 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
      961 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     1021 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     1081 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     1141 tttttttctt ccatttcagg tgtcgtgaag cggccctgca gatatcaaca agtttgtaca
     1201 aaaaagcagg caccatggcg gcgctgagcg gtggcggtgg tggcggcgcg gagccgggcc
     1261 aggctctgtt caacggggac atggagcccg aggccggcgc cggcgccggc gccgcggcct
     1321 cttcggctgc ggaccctgcc attccggagg aggtgtggaa tatcaaacaa atgattaagt
     1381 tgacacagga acatatagag gccctattgg acaaatttgg tggggagcat aatccaccat
     1441 caatatatct ggaggcctat gaagaataca ccagcaagct agatgcactc caacaaagag
     1501 aacaacagtt attggaatct ctggggaacg gaactgattt ttctgtttct agctctgcat
     1561 caatggatac cgttacatct tcttcctctt ctagcctttc agtgctacct tcatctcttt
     1621 cagtttttca aaatcccaca gatgtggcac ggagcaaccc caagtcacca caaaaaccta
     1681 tcgttagagt cttcctgccc aacaaacaga ggacagtggt acctgcaagg tgtggagtta
     1741 cagtccgaga cagtctaaag aaagcactga tgatgagagg tctaatccca gagtgctgtg
     1801 ctgtttacag aattcaggat ggagagaaga aaccaattgg ttgggacact gatatttcct
     1861 ggcttactgg agaagaattg catgtggaag tgttggagaa tgttccactt acaacacaca
     1921 actttgtacg aaaaacgttt ttcaccttag cattttgtga cttttgtcga aagctgcttt
     1981 tccagggttt ccgctgtcaa acatgtggtt ataaatttca ccagcgttgt agtacagaag
     2041 ttccactgat gtgtgttaat tatgaccaac ttgatttgct gtttgtctcc aagttctttg
     2101 aacaccaccc aataccacag gaagaggcgt ccttagcaga gactgcccta acatctggat
     2161 catccccttc cgcacccgcc tcggactcta ttgggcccca aattctcacc agtccgtctc
     2221 cttcaaaatc cattccaatt ccacagccct tccgaccagc agatgaagat catcgaaatc
     2281 aatttgggca acgagaccga tcctcatcag ctcccaatgt gcatataaac acaatagaac
     2341 ctgtcaatat tgatgacttg attagagacc aaggatttcg tggtgatgga ggatcaacca
     2401 caggtttgtc tgctaccccc cctgcctcat tacctggctc actaactaac gtgaaagcct
     2461 tacagaaatc tccaggacct cagcgagaaa ggaagtcatc ttcatcctca gaagacagga
     2521 atcgaatgaa aacacttggt agacgggact cgagtgatga ttgggagatt cctgatgggc
     2581 agattacagt gggacaaaga attggatctg gatcatttgg aacagtctac aagggaaagt
     2641 ggcatggtga tgtggcagtg aaaatgttga atgtgacagc acctacacct cagcagttac
     2701 aagccttcaa aaatgaagta ggagtactca ggaaaacacg acatgtgaat atcctactct
     2761 tcatgggcta ttccacaaag ccacaactgg ctattgttac ccagtggtgt gagggctcca
     2821 gcttgtatca ccatctccat atcattgaga ccaaatttga gatgatcaaa cttatagata
     2881 ttgcacgaca gactgcacag ggcatggatt acttacacgc caagtcaatc atccacagag
     2941 acctcaagag taataatata tttcttcatg aagacctcac agtaaaaata ggtgattttg
     3001 gtctagctac agtgaaatct cgatggagtg ggtcccatca gtttgaacag ttgtctggat
     3061 ccattttgtg gatggcacca gaagtcatca gaatgcaaga taaaaatcca tacagctttc
     3121 agtcagatgt atatgcattt ggaattgttc tgtatcaatt gatgactgga cagttacctt
     3181 attcaaacat caacaacagg gaccagataa tttttatggt gggacgagga tacctgtctc
     3241 cagatctcag taaggtacgg agtaactgtc caaaagccat gaagagatta atggcagagt
     3301 gcctcaaaaa gaaaagagat gagagaccac tctttcccca aattctcgcc tctattgagc
     3361 tgctggcccg ctcattgcca aaaattcacc gcagtgcatc agaaccctcc ttgaatcggg
     3421 ctggtttcca aacagaggat tttagtctat atgcttgtgc ttctccaaaa acacccatcc
     3481 aggcaggggg atatggtgcg tttcctgtcc actagaaccc agctttcttg tacaaagtgg
     3541 tgatatccaa ttaaccctca ctaaaggttg tatgtgagtt tagattaatg accctatagt
     3601 gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca cagtggcggc
     3661 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     3721 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc
     3781 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3841 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3901 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3961 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     4021 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     4081 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     4141 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4201 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4261 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4321 tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4381 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4441 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4501 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4561 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4621 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4681 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4741 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4801 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4861 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4921 gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     4981 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5041 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5101 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5161 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5221 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5281 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5341 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5401 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5461 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5521 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     5581 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5641 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5701 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5761 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5821 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     5881 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     5941 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6001 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6361 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     6421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6661 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6841 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7321 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7801 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7861 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7921 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7981 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
     8041 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8101 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
     8161 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8221 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8281 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
     8341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     8401 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     8461 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     8521 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
     8581 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     8641 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     8701 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     8761 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     8821 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     8881 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     8941 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     9001 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9061 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9121 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9181 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     9241 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9301 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9361 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9421 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9481 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9541 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9601 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9661 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     9721 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9781 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9841 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9901 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9961 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    10021 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    10081 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    10141 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    10201 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    10261 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    10321 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    10381 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    10441 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    10501 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    10561 tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa
    10621 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
    10681 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
    10741 ttacagggac agcagagatc cagtttggac tagtcgtgag gctccggtgc
//
