LOCUS       sequence-237687-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF K499N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-K499N
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..1188
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          241..1179
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1136..1156
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1208..1232
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1233..1242
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3537..3561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3569..3587
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3612..3631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3613..3631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3637..3657)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3711..3752
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3773..4340
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3790..4342
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3934..3951)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4161..4180
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4354..4953
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4354..4373)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4850..4870
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4967..5555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5020..5040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5438..5449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5630..5863
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5982..6000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6068..6172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6173..6241,6242..7033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6391..6410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7204..7792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7693..7712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7946..7963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8203..8224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8239..8244,8245..8262,8263..8269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8277..8293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8282..8304
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8301..8317
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8301..8317
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8367..8386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8578..9211
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9258..9383
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9880..10113
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10298..10342
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10635..10752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
       61 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      121 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      181 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      241 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
      301 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      421 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      961 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1021 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1081 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1141 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccctgca
     1201 gatatcaaca agtttgtaca aaaaagcagg caccatggcg gcgctgagcg gtggcggtgg
     1261 tggcggcgcg gagccgggcc aggctctgtt caacggggac atggagcccg aggccggcgc
     1321 cggcgccggc gccgcggcct cttcggctgc ggaccctgcc attccggagg aggtgtggaa
     1381 tatcaaacaa atgattaagt tgacacagga acatatagag gccctattgg acaaatttgg
     1441 tggggagcat aatccaccat caatatatct ggaggcctat gaagaataca ccagcaagct
     1501 agatgcactc caacaaagag aacaacagtt attggaatct ctggggaacg gaactgattt
     1561 ttctgtttct agctctgcat caatggatac cgttacatct tcttcctctt ctagcctttc
     1621 agtgctacct tcatctcttt cagtttttca aaatcccaca gatgtggcac ggagcaaccc
     1681 caagtcacca caaaaaccta tcgttagagt cttcctgccc aacaaacaga ggacagtggt
     1741 acctgcaagg tgtggagtta cagtccgaga cagtctaaag aaagcactga tgatgagagg
     1801 tctaatccca gagtgctgtg ctgtttacag aattcaggat ggagagaaga aaccaattgg
     1861 ttgggacact gatatttcct ggcttactgg agaagaattg catgtggaag tgttggagaa
     1921 tgttccactt acaacacaca actttgtacg aaaaacgttt ttcaccttag cattttgtga
     1981 cttttgtcga aagctgcttt tccagggttt ccgctgtcaa acatgtggtt ataaatttca
     2041 ccagcgttgt agtacagaag ttccactgat gtgtgttaat tatgaccaac ttgatttgct
     2101 gtttgtctcc aagttctttg aacaccaccc aataccacag gaagaggcgt ccttagcaga
     2161 gactgcccta acatctggat catccccttc cgcacccgcc tcggactcta ttgggcccca
     2221 aattctcacc agtccgtctc cttcaaaatc cattccaatt ccacagccct tccgaccagc
     2281 agatgaagat catcgaaatc aatttgggca acgagaccga tcctcatcag ctcccaatgt
     2341 gcatataaac acaatagaac ctgtcaatat tgatgacttg attagagacc aaggatttcg
     2401 tggtgatgga ggatcaacca caggtttgtc tgctaccccc cctgcctcat tacctggctc
     2461 actaactaac gtgaaagcct tacagaaatc tccaggacct cagcgagaaa ggaagtcatc
     2521 ttcatcctca gaagacagga atcgaatgaa aacacttggt agacgggact cgagtgatga
     2581 ttgggagatt cctgatgggc agattacagt gggacaaaga attggatctg gatcatttgg
     2641 aacagtctac aagggaaagt ggcatggtga tgtggcagtg aaaatgttga atgtgacagc
     2701 acctacacct cagcagttac aagccttcaa taatgaagta ggagtactca ggaaaacacg
     2761 acatgtgaat atcctactct tcatgggcta ttccacaaag ccacaactgg ctattgttac
     2821 ccagtggtgt gagggctcca gcttgtatca ccatctccat atcattgaga ccaaatttga
     2881 gatgatcaaa cttatagata ttgcacgaca gactgcacag ggcatggatt acttacacgc
     2941 caagtcaatc atccacagag acctcaagag taataatata tttcttcatg aagacctcac
     3001 agtaaaaata ggtgattttg gtctagctac agtgaaatct cgatggagtg ggtcccatca
     3061 gtttgaacag ttgtctggat ccattttgtg gatggcacca gaagtcatca gaatgcaaga
     3121 taaaaatcca tacagctttc agtcagatgt atatgcattt ggaattgttc tgtatgaatt
     3181 gatgactgga cagttacctt attcaaacat caacaacagg gaccagataa tttttatggt
     3241 gggacgagga tacctgtctc cagatctcag taaggtacgg agtaactgtc caaaagccat
     3301 gaagagatta atggcagagt gcctcaaaaa gaaaagagat gagagaccac tctttcccca
     3361 aattctcgcc tctattgagc tgctggcccg ctcattgcca aaaattcacc gcagtgcatc
     3421 agaaccctcc ttgaatcggg ctggtttcca aacagaggat tttagtctat atgcttgtgc
     3481 ttctccaaaa acacccatcc aggcaggggg atatggtgcg tttcctgtcc actagaaccc
     3541 agctttcttg tacaaagtgg tgatatccaa ttaaccctca ctaaaggtat ttgataagag
     3601 aatgaagaag tccctatagt gagtcgtatt accacccaac ttttctatac aaagtggttg
     3661 atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta
     3721 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gatccctccc
     3781 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     3841 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     3901 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     3961 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4021 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4081 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     4141 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     4201 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     4261 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     4321 tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc cacggtgcgc
     4381 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     4441 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     4501 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     4561 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     4621 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     4681 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     4741 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     4801 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     4861 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     4921 acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc aacctctgga
     4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5341 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     5581 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5641 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5701 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5761 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5821 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5881 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     5941 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     6001 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     6421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7801 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7861 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7921 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7981 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
     8041 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8101 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
     8161 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8221 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8281 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     8341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8401 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     8461 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     8521 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     8581 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     8641 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     8701 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     8761 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8821 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8881 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8941 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     9001 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     9061 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9121 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9181 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9241 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9301 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9361 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9421 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9481 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9541 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9601 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9661 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9721 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9781 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9841 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9901 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9961 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    10021 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    10081 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    10141 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    10201 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    10261 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    10321 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10381 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10441 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10501 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    10561 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
    10621 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10681 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10741 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac
//
