LOCUS       sequence-237688-       10432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mTFP under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP609
FEATURES             Location/Qualifiers
     source          1..10432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1509..1525)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1509..1525)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1532..1556)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2984..3004)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3032..3228)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3234..4049)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3234..4049)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3957..3976
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4059..4081)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4070..4086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4095..4100,4101..4116,4117..4122))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      4477..5065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4966..4985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5128..6291
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6314..6334
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7670..7694
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7840..7851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9791..9808
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9792..9808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9833..10432
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 ctttgctcta gaatggtttc taagggagag gagaccacca tgggagtcat caagccagac
       61 atgaagatca agctcaagat ggagggaaac gtcaacggac acgccttcgt catcgaggga
      121 gagggagagg gaaagccata cgacggaacc aacactatca acctcgaggt caaggaagga
      181 gccccactcc cattctccta cgacatcctc accaccgcgt tcgcctacgg aaaccgtgcc
      241 ttcaccaagt acccagacga catcccaaac tactttaagc aatccttccc agagggatac
      301 tcctgggagc gtaccatgac cttcgaagac aagggaatcg tcaaggtcaa gtcagacatc
      361 tccatggagg aggactcctt catctacgag atccacctca agggagagaa cttcccacca
      421 aacggaccag tcatgcaaaa gaagaccacc ggatgggacg cctccaccga gagaatgtac
      481 gtccgtgacg gagtcctcaa gggagacgtc aagcacaagt tgctcctcga gggaggagga
      541 caccaccgtg tcgactttaa gaccatctac cgtgccaaga aggccgtcaa gctcccagac
      601 taccacttcg tcgaccaccg tatagagatc ctcaaccacg acaaggacta caacaaggtc
      661 accgtctacg agtccgccgt cgcccgtaac tctaccgacg gaatggatga gctctacaag
      721 taaggccgct gtcatcagac atctcgcgcc cgtgcctctg acttctaagt ccaattactc
      781 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
      841 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
      901 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
      961 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     1021 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     1081 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     1141 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     1201 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta
     1261 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     1321 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     1381 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     1441 ttcccaatat accaaacata actgtttgcc cactagtgag tcgtattacg tagcttggcg
     1501 taatcatggt catagctgtt tcctgcccgt gacccagctt tcttgtacaa agtggtaagt
     1561 gcaagtaaga tcagtgtttg tttcgatatc agtctgtttc gtaacggtct tctgtataac
     1621 tacaaaaaag aataaaaaac cgtatcacaa ctgagaaaaa agacaaaact tattgaacag
     1681 tgttcctttc aaaaaattat tttgtaaaaa atcgagtttt tctccaaaat aacgcacgca
     1741 atcaaaaaca agaaaactaa cgtgttctcc tgggcgtgga actccttatc aagattacca
     1801 ttattgtctg tcagaattga tatttcaatt atttcatatc tagacaaacc cgggtgtctt
     1861 gcatttatta gtactgtgag tttacataca aacgaggctt actcagataa catagagtca
     1921 cctctaatta gcaaaaaccc atttctaatt agctatttcg accagttcta aagtcaccgt
     1981 aattagaaat ggtttttgta gcttttctaa ttagaaatga ccccgagata agtgaaatat
     2041 ggcattttct gggttttcta gtaagaaatg atcccgcacg tagtgattcc gcgttacaag
     2101 ccaaattcaa attaaaaaag tatattacac aattcaagtt acattatttt caggaaacaa
     2161 tggcgaagag caaagcagag attcaaaagg aggaaagaga caacagatat gaacgaagac
     2221 ttcttgaaaa gagaaagaga catgaggtga gtaaccatga taataaataa tcaagtaagg
     2281 gctatagatt tataaattct ggagttaaca gtttcgaaaa aaagtagttt tcatatgtgc
     2341 gaaggaatat caaaaatagg ggaatagcct aaacgagtga gaaaattaaa tgtttgttat
     2401 tttttcaaaa atgtctgcag ggccgttatc ctttcaaatg cactctatca aacatagatc
     2461 atatccatca tatcttatcc agaatttata tcatatccaa gattgagagc gtttcaagga
     2521 aaatctcaaa caaccataca attttgtaga tcgacaacaa ggcctccact tcttcatcga
     2581 tggcctctgc agcacctgca ccaccaagtt tgtatttttc atattaatat aaagtgagcc
     2641 acgcatagtc gtttttagga tttttgccag atcgatgtcg cgcaaacaga ctgttttttt
     2701 ttctttgaaa gccgtgcgtg acatgggcca cggattgtaa cgccgcgctg gatggataaa
     2761 aatttattca gttttttagc ctttacaaaa tatggttcga ctactttctt tatttttaca
     2821 tttttatagg tgctcattga gctgagatat tttgggtata atcggaaaat cgctgatttt
     2881 ggtcattttt ctgcactaaa gattacacaa aaagttcagt atttagattc attgttgaaa
     2941 attggggatg atctacagat acatatcgcc gtgttcaaaa acacaacttt attatacata
     3001 gttgataatt cgatggtatc agctcactca aagtatagcg accagcattc acatacgatt
     3061 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
     3121 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
     3181 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattagaaa
     3241 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     3301 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     3361 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     3421 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     3481 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     3541 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     3601 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     3661 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     3721 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     3781 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     3841 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     3901 ggcgcatcgg gcttaccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     3961 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag
     4021 caagacgttt cccgttgaat atggctcatt ttatgtttcc tgtgtgaaat tgttatccgc
     4081 tcacaattcc acagattata cgagccgatg attaattgtc aacagctctt tatgttcgga
     4141 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     4201 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     4261 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     4321 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     4381 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     4441 aaggagggta ttctgggcct ccatgtcggg atcttcttga gatccttttt ttctgcgcgt
     4501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4621 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5101 cttttgctgg ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa
     5161 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     5221 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     5281 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     5341 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     5401 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     5461 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     5521 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     5581 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     5641 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     5701 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     5761 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     5821 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     5881 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     5941 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     6001 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
     6061 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata
     6121 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     6181 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     6241 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gcgccaggga
     6301 tccgccaagc tatcaacttt gtatagaaaa gttgtgtcgg attatgggct cttcttttat
     6361 cctctacttt ctttctagaa aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa
     6421 ctgcaatggg tttagcttat gtttctggaa caattactgg tggaattgtg attgaaaagt
     6481 aaggttatat ttcatttcaa taaatgttac cacagtataa caaaaacaag ttacattgtt
     6541 cagagtatgt ctgaatgtat tattattatt gtatttgtgc atcagtactg tagttgtact
     6601 gaagaagggt ttaaaataat ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca
     6661 gttaggatgt ttggacatag ttttattaga taatcgtgaa ctaactcact ataaaaaccc
     6721 gctaactcat tgaataagat agttttaaac gttcaaaatt tgaagacaaa attgtatgaa
     6781 taagtttaca gagttggtcg acgcaagctt ctccttctct tcacatttct caataattta
     6841 gctctaattg cattcgtatt cttcgcaaaa atacgtattc tgattgaccc gatgaaatac
     6901 ggatgcctag ttgcattgat catctacggt tatacttatg ggtgaggttt ttcaaatata
     6961 ttttgattta atcgaataat gaaatttcag aactggagtt ggtccaattt cgtggtttat
     7021 atcctctgaa ctggtacctc aaaaacatcg tagtattgct caatctgtgg catattcaat
     7081 gaatactgta atggtggtta tcagtacatt tacagtatta cctctatatt ccgttattgg
     7141 tagttatgct ttccttattc tatattcaat tccttcattt ataagtatgc ttatattatt
     7201 tcgctactta ccggaaacca aaggacgaga gattcatgat attgtgaatg aacttaaaaa
     7261 aaaatgaaaa cctttgaaaa cgttcatacg gccgaaattt tacttgatcg agccaattga
     7321 acttttgtta agacttacag aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc
     7381 gaaaatgaac aggaaaaaga agaaaaatac acccgaagta tcatataaaa attaaaaaga
     7441 ttgtttgacc tggcggaact tttttgcaaa aactctgaat atccatggca catgcttcgt
     7501 gcaaaacagg atccaaatga atatcaacaa gagattcttg aagacgacga gccacttgct
     7561 cagagcattg cttatctcga atgagaccct tgttaaaatc atttttcaaa cattatatga
     7621 cattgtcgaa atgtcctcct gattccatga tggtagcaaa ctcacttcgt caagtttgta
     7681 caaaaaagca ggcttgagcc aatttatcca agtccttgta aaaagtattc gaaaattgtt
     7741 aacggataaa atgtttatta taatatcaaa aacaatttgt cagttgacca ctttttgata
     7801 tatttgacag aaaccgggat gaattggctc aaagttaggg cgcccttcta ttacagggtt
     7861 tctgataaca aaccggttat taactcccaa caagggatga tttcaattca tcatgctcaa
     7921 attgacccaa attaagttac atgacaaatt catcgccctt ttcaactctt cttggctcat
     7981 catctgttat tctgttccta ttctgtctgc accctatacc cctttgcata ctctctcgtc
     8041 attcctttct tggatagtgt cttcttctcc ccagctctgc tacttctatg acttgcgccc
     8101 gctgcttttc cgctcgttcc tctcctctct gacgtcttct tctgcttctt ttctccttcc
     8161 tttttccctt ctcaatcttc ctctttttcc catttctgtc aatcattcga agaagaagaa
     8221 gaagaagacc ctcattcatt tcattttttt ttctgtcggt gtgtgctgct cggttaagag
     8281 tgagctctct attccacgtc ttcttctttt tcttcttcga ttcgaatcaa tcactccacc
     8341 aaaacgcatt cgtttttggg attcaccccg cggttcgcaa taggtttctt tttcaaatta
     8401 ttagcgttat aaatagaaaa atggtggagt ttcaataaaa atgataattt acaaagtgta
     8461 ttttgattac atgtactcaa aaaggttgaa atttttcata ccagttttcc ggaaatccat
     8521 ctgatatcat tatcgtattt ttctttttaa aaatgttttc aaaaaaaaac aaaatatagc
     8581 tggttatttg gcaccctcta attaccattt tcctgtcaca ccacactctt ttccttcttc
     8641 cacttctttc cgttttcagc cgcttccaac caaaccgata tgaaagccga gcaacaacga
     8701 tcgattccac ccggctcggc gaccttcccg tcgcaggtga gactcagaaa actagagaaa
     8761 ccgctcaact aactcttgat atccgatttc attcttttct cttttctttt tgttgaactt
     8821 ttccatattt ccagatgcca cggccaccac caagcaccga acaaggaatc acaacggaat
     8881 cggagcttgc gaagaaagct caaatcactc cgaacgacgt tttagcactt ccgggaatca
     8941 ctcaaggtat cttgctccct ttggatttca gtttaaaaca taaatttagg attcttatgc
     9001 tccccatctg cgaacatcta taacatcgag ttcaccaagt tccaaatccg tgatctggac
     9061 actgagcaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg
     9121 ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt gaaactcaaa
     9181 acggtcggag caactgtgga gttcaaagta ggagacatcc caatccatca tttccgaatg
     9241 atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga atttggattc
     9301 tgtattccga actcacgaaa caactgtgaa catatctatg agttccctca actctctcaa
     9361 caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc tttttcagtg
     9421 gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt cgtcgataac
     9481 aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatatt taatacaaaa
     9541 atgttctgga taattattct gtcgaataga aaaaaaactc caaatgtgat taaattccaa
     9601 taattcctgt ctagtttgtt ccttcccctt ccccttctca tgttcaatgc attcctaagc
     9661 ttttcagttc cccccttgtt tctatatttt ttcgctgtcc tgtcacactc gctaaaaaca
     9721 ctaatcacac ggaaatctgt tttcaataaa aactccaact ttaactcatt ttcaatttca
     9781 actgcacggg tgtaaaacga cggccagtcc gtaatacgac tcacttaagg ccgcaccttt
     9841 ggtcttttat tgtcaacttc cattggttct tccattgttt ctgttaaatt aatgaatttt
     9901 tcataaaata aagacattat acaatataaa aatgaagaat ttattgaaaa taaactgcca
     9961 gagagaaaaa gtatgcaaca ctcccgccga gagtgtttga aatggtgtac ggtacatttt
    10021 cgtgctagga gttagatgtg caggcagcaa cgagaggggg agagattttt ttgggccttg
    10081 tgaaattaac gtgagttttc tggtcatctg actaatcatg ttggtttttt gttggtttat
    10141 tttgttttta tctttgtttt tatccagatt aggaaattta aattttatga atttataatg
    10201 aggtcaaaca ttcagtccca gcgtttttcc tgttctcact gtttagtcga atttttattt
    10261 taggctttca acaaatgttc taactgtctt atttgtgacc tcacttttta tattttttta
    10321 atttttaaaa atattagaag tttctaggat aattttttcg acttttattc tctctaccgt
    10381 ccgcactctt cttactttta aattaaattg tttttttttc agttgggaaa ca
//
