LOCUS       sequence-237689-       10379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mKO2 under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP607
FEATURES             Location/Qualifiers
     source          1..10379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..669
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(1455..1471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1455..1471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1478..1502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2931..2951)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(2979..3175)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3181..3996)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3181..3996)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3904..3923
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4006..4028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4017..4033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4042..4047,4048..4063,4064..4069))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      4424..5012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4913..4932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5075..6238
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6261..6281
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7617..7641
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7787..7798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9738..9755
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9739..9755
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9780..10379
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 ctttgctcta gaatggtctc cgtcatcaag ccagagatga agatgcgtta ctacatggac
       61 ggctccgtca acggacacga gttcacaatc gagggagagg gaaccggacg tccatacgag
      121 ggacaccaag agatgaccct ccgtgtcacc atggccgagg gagggccaat gccattcgcc
      181 ttcgacctcg tgtcccacgt cttctgctac ggacaccgtg tcttcaccaa gtacccagag
      241 gagatcccag actacttcaa gcaagccttc ccagagggac tctcctggga gcgttccctc
      301 gagttcgagg acggaggatc cgcctccgtc tccgcgcaca tctccctccg tggaaacacc
      361 ttctaccaca agtccaagtt caccggagtc aacttcccag ccgacggacc aatcatgcaa
      421 aaccaatccg tcgactggga gccatccacc gagaagatca ccgcctccga cggagtcctc
      481 aagggagacg tcaccatgta cctcaagctc gagggaggag gaaaccacaa gtgccaaatg
      541 aagaccacct acaaggccgc caaggagatc ctcgagatgc caggagacca ctacatcgga
      601 caccgtctcg tccgtaagac cgagggaaac atcaccgagc aagtcgagga cgccgtcgcc
      661 cacagctaag gccgctgtca tcagacatct cgcgcccgtg cctctgactt ctaagtccaa
      721 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
      781 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
      841 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
      901 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
      961 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     1021 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt
     1081 ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga
     1141 atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt
     1201 cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     1261 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     1321 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     1381 aatacattcc caatatacca aacataactg tttgcccact agtgagtcgt attacgtagc
     1441 ttggcgtaat catggtcata gctgtttcct gcccgtgacc cagctttctt gtacaaagtg
     1501 gtaagtgcaa gtaagatcag tgtttgtttc gatatcagtc tgtttcgtaa cggtcttctg
     1561 tataactaca aaaaagaata aaaaaccgta tcacaactga gaaaaaagac aaaacttatt
     1621 gaacagtgtt cctttcaaaa aattattttg taaaaaatcg agtttttctc caaaataacg
     1681 cacgcaatca aaaacaagaa aactaacgtg ttctcctggg cgtggaactc cttatcaaga
     1741 ttaccattat tgtctgtcag aattgatatt tcaattattt catatctaga caaacccggg
     1801 tgtcttgcat ttattagtac tgtgagttta catacaaacg aggcttactc agataacata
     1861 gagtcacctc taattagcaa aaacccattt ctaattagct atttcgacca gttctaaagt
     1921 caccgtaatt agaaatggtt tttgtagctt ttctaattag aaatgacccc gagataagtg
     1981 aaatatggca ttttctgggt tttctagtaa gaaatgatcc cgcacgtagt gattccgcgt
     2041 tacaagccaa attcaaatta aaaaagtata ttacacaatt caagttacat tattttcagg
     2101 aaacaatggc gaagagcaaa gcagagattc aaaaggagga aagagacaac agatatgaac
     2161 gaagacttct tgaaaagaga aagagacatg aggtgagtaa ccatgataat aaataatcaa
     2221 gtaagggcta tagatttata aattctggag ttaacagttt cgaaaaaaag tagttttcat
     2281 atgtgcgaag gaatatcaaa aataggggaa tagcctaaac gagtgagaaa attaaatgtt
     2341 tgttattttt tcaaaaatgt ctgcagggcc gttatccttt caaatgcact ctatcaaaca
     2401 tagatcatat ccatcatatc ttatccagaa tttatatcat atccaagatt gagagcgttt
     2461 caaggaaaat ctcaaacaac catacaattt tgtagatcga caacaaggcc tccacttctt
     2521 catcgatggc ctctgcagca cctgcaccac caagtttgta tttttcatat taatataaag
     2581 tgagccacgc atagtcgttt ttaggatttt tgccagatcg atgtcgcgca aacagactgt
     2641 ttttttttct ttgaaagccg tgcgtgacat gggccacgga ttgtaacgcc gcgctggatg
     2701 gataaaaatt tattcagttt tttagccttt acaaaatatg gttcgactac tttctttatt
     2761 tttacatttt tataggtgct cattgagctg agatattttg ggtataatcg aaaaaatcgc
     2821 tgattttggt catttttctg cactaaagat tacacaaaaa gttcagtatt tagattcatt
     2881 gttgaaaatt ggggatgatc tacagataca tatcgccgtg ttcaaaaaca caactttatt
     2941 atacatagtt gataattcga tggtatcagc tcactcaaag tatagcgacc agcattcaca
     3001 tacgattgac gcatgatatt actttctgcg cacttaactt cgcatctggg cagatgatgt
     3061 cgaggcgaaa aaaaatataa atcacgctaa catttgatta aaatagaaca actacaatat
     3121 aaaaaaacta tacaaatgac aagttcttga aaacaagaat ctttttattg tcagtactga
     3181 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     3241 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     3301 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     3361 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     3421 tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca
     3481 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     3541 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     3601 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     3661 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     3721 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     3781 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     3841 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     3901 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     3961 cctcgagcaa gacgtttccc gttgaatatg gctcatttta tgtttcctgt gtgaaattgt
     4021 tatccgctca caattccaca gattatacga gccgatgatt aattgtcaac agctctttat
     4081 gttcggatgt gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga
     4141 agaacctcag tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac
     4201 aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt
     4261 aatttttgct tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg
     4321 gatgtatggg ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa
     4381 gactgtcaag gagggtattc tgggcctcca tgtcgggatc ttcttgagat cctttttttc
     4441 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4501 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4561 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4621 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4681 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4741 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4801 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4861 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4921 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4981 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5041 tcctggcctt ttgctggccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt
     5101 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     5161 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
     5221 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     5281 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     5341 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     5401 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     5461 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     5521 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     5581 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     5641 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     5701 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     5761 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     5821 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     5881 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     5941 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
     6001 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagcta
     6061 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
     6121 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
     6181 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgcg
     6241 ccagggatcc gccaagctat caactttgta tagaaaagtt gtgtcggatt atgggctctt
     6301 cttttatcct ctactttctt tctagaaaat gcaaatgttg agtcagaaat tgctgaatgg
     6361 agtacaactg caatgggttt agcttatgtt tctggaacaa ttactggtgg aattgtgatt
     6421 gaaaagtaag gttatatttc atttcaataa atgttaccac agtataacaa aaacaagtta
     6481 cattgttcag agtatgtctg aatgtattat tattattgta tttgtgcatc agtactgtag
     6541 ttgtactgaa gaagggttta aaataatctc aacgttttta agacgagtta gtgagaatac
     6601 ggtcgcagtt aggatgtttg gacatagttt tattagataa tcgtgaacta actcactata
     6661 aaaacccgct aactcattga ataagatagt tttaaacgtt caaaatttga agacaaaatt
     6721 gtatgaataa gtttacagag ttggtcgacg caagcttctc cttctcttca catttctcaa
     6781 taatttagct ctaattgcat tcgtattctt cgcaaaaata cgtattctga ttgacccgat
     6841 gaaatacgga tgcctagttg cattgatcat ctacggttat acttatgggt gaggtttttc
     6901 aaatatattt tgatttaatc gaataatgaa atttcagaac tggagttggt ccaatttcgt
     6961 ggtttatatc ctctgaactg gtacctcaaa aacatcgtag tattgctcaa tctgtggcat
     7021 attcaatgaa tactgtaatg gtggttatca gtacatttac agtattacct ctatattccg
     7081 ttattggtag ttatgctttc cttattctat attcaattcc ttcatttata agtatgctta
     7141 tattatttcg ctacttaccg gaaaccaaag gacgagagat tcatgatatt gtgaatgaac
     7201 ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc gaaattttac ttgatcgagc
     7261 caattgaact tttgttaaga cttacagaaa agtgagagat tttgttacaa aaaagtgaga
     7321 aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc cgaagtatca tataaaaatt
     7381 aaaaagattg tttgacctgg cggaactttt ttgcaaaaac tctgaatatc catggcacat
     7441 gcttcgtgca aaacaggatc caaatgaata tcaacaagag attcttgaag acgacgagcc
     7501 acttgctcag agcattgctt atctcgaatg agacccttgt taaaatcatt tttcaaacat
     7561 tatatgacat tgtcgaaatg tcctcctgat tccatgatgg tagcaaactc acttcgtcaa
     7621 gtttgtacaa aaaagcaggc ttgagccaat ttatccaagt ccttgtaaaa agtattcgaa
     7681 aattgttaac ggataaaatg tttattataa tatcaaaaac aatttgtcag ttgaccactt
     7741 tttgatatat ttgacagaaa ccgggatgaa ttggctcaaa gttagggcgc ccttctatta
     7801 cagggtttct gataacaaac cggttattaa ctcccaacaa gggatgattt caattcatca
     7861 tgctcaaatt gacccaaatt aagttacatg acaaattcat cgcccttttc aactcttctt
     7921 ggctcatcat ctgttattct gttcctattc tgtctgcacc ctatacccct ttgcatactc
     7981 tctcgtcatt cctttcttgg atagtgtctt cttctcccca gctctgctac ttctatgact
     8041 tgcgcccgct gcttttccgc tcgttcctct cctctctgac gtcttcttct gcttcttttc
     8101 tccttccttt ttcccttctc aatcttcctc tttttcccat ttctgtcaat cattcgaaga
     8161 agaagaagaa gaagaccctc attcatttca tttttttttc tgtcggtgtg tgctgctcgg
     8221 ttaagagtga gctctctatt ccacgtcttc ttctttttct tcttcgattc gaatcaatca
     8281 ctccaccaaa acgcattcgt ttttgggatt caccccgcgg ttcgcaatag gtttcttttt
     8341 caaattatta gcgttataaa tagaaaaatg gtggagtttc aataaaaatg ataatttaca
     8401 aagtgtattt tgattacatg tactcaaaaa ggttgaaatt tttcatacca gttttccgga
     8461 aatccatctg atatcattat cgtatttttc tttttaaaaa tgttttcaaa aaaaaacaaa
     8521 atatagctgg ttatttggca ccctctaatt accattttcc tgtcacacca cactcttttc
     8581 cttcttccac ttctttccgt tttcagccgc ttccaaccaa accgatatga aagccgagca
     8641 acaacgatcg attccacccg gctcggcgac cttcccgtcg caggtgagac tcagaaaact
     8701 agagaaaccg ctcaactaac tcttgatatc cgatttcatt cttttctctt ttctttttgt
     8761 tgaacttttc catatttcca gatgccacgg ccaccaccaa gcaccgaaca aggaatcaca
     8821 acggaatcgg agcttgcgaa gaaagctcaa atcactccga acgacgtttt agcacttccg
     8881 ggaatcactc aaggtatctt gctccctttg gatttcagtt taaaacataa atttaggatt
     8941 cttatgctcc ccatctgcga acatctataa catcgagttc accaagttcc aaatccgtga
     9001 tctggacact gagcaagtgc ttttcgagat cgccaaaccg gagaacgatc aggagaatga
     9061 tgagtcgcca caggagtcgg caagatacgt gcgttataga tttgctccaa actttttgaa
     9121 actcaaaacg gtcggagcaa ctgtggagtt caaagtagga gacatcccaa tccatcattt
     9181 ccgaatgatc gaacgtcact tcttcaaaga tcgccttctg aagtgttttg actttgaatt
     9241 tggattctgt attccgaact cacgaaacaa ctgtgaacat atctatgagt tccctcaact
     9301 ctctcaacaa ctcagtgagt cattattcta aaaagtacaa ttcaaaagac taatctcttt
     9361 ttcagtggac gacatgatca acaatccaaa cgagactcgt tctgacagct tctatttcgt
     9421 cgataacaaa ctcgtcatgc acaacaaagc cgactactca tatgatgcat aaatatttaa
     9481 tacaaaaatg ttctggataa ttattctgtc gaatagaaaa aaaactccaa atgtgattaa
     9541 attccaataa ttcctgtcta gtttgttcct tccccttccc cttctcatgt tcaatgcatt
     9601 cctaagcttt tcagttcccc ccttgtttct atattttttc gctgtcctgt cacactcgct
     9661 aaaaacacta atcacacgga aatctgtttt caataaaaac tccaacttta actcattttc
     9721 aatttcaact gcacgggtgt aaaacgacgg ccagtccgta atacgactca cttaaggccg
     9781 cacctttggt cttttattgt caacttccat tggttcttcc attgtttctg ttaaattaat
     9841 gaatttttca taaaataaag acattataca atataaaaat gaagaattta ttgaaaataa
     9901 actgccagag agaaaaagta tgcaacactc ccgccgagag tgtttgaaat ggtgtacggt
     9961 acattttcgt gctaggagtt agatgtgcag gcagcaacga gagggggaga gatttttttg
    10021 ggccttgtga aattaacgtg agttttctgg tcatctgact aatcatgttg gttttttgtt
    10081 ggtttatttt gtttttatct ttgtttttat ccagattagg aaatttaaat tttatgaatt
    10141 tataatgagg tcaaacattc agtcccagcg tttttcctgt tctcactgtt tagtcgaatt
    10201 tttattttag gctttcaaca aatgttctaa ctgtcttatt tgtgacctca ctttttatat
    10261 ttttttaatt tttaaaaata ttagaagttt ctaggataat tttttcgact tttattctct
    10321 ctaccgtccg cactcttctt acttttaaat taaattgttt ttttttcagt tgggaaaca
//
