LOCUS       sequence-237690-       10492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mCerulean under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP605
FEATURES             Location/Qualifiers
     source          1..10492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1963..1979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1963..1979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1986..2010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3438..3458)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3486..3682)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3688..4503)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3688..4503)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4411..4430
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4513..4535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4524..4540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4549..4554,4555..4570,4571..4576))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      4931..5519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5420..5439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5582..6745
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6768..6788
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8124..8148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8294..8305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10245..10262
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10246..10262
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(10287..10492,1..394)
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 tgcaggcagc aacgagaggg ggagagattt ttttgggcct tgtgaaatta acgtgagttt
       61 tctggtcatc tgactaatca tgttggtttt ttgttggttt attttgtttt tatctttgtt
      121 tttatccaga ttaggaaatt taaattttat gaatttataa tgaggtcaaa cattcagtcc
      181 cagcgttttt cctgttctca ctgtttagtc gaatttttat tttaggcttt caacaaatgt
      241 tctaactgtc ttatttgtga cctcactttt tatatttttt taatttttaa aaatattaga
      301 agtttctagg ataatttttt cgacttttat tctctctacc gtccgcactc ttcttacttt
      361 taaattaaat tgtttttttt tcagttggga aacactttgc tctagaatgg tttctaaggg
      421 agaggagctt ttcactggag tggtcccaat ccttgtcgag cttgatggag atgttaacgg
      481 acacaagttc tctgtttctg gagagggaga gggagacgct acctacggaa agcttactct
      541 taagttcatc tgcactactg gaaagcttcc agttccatgg ccaacccttg ttaccaccct
      601 ttcttgggga gttcaatgct tcgctcgtta cccagatcat atgaagcaac atgatttctt
      661 caagtctgct atgccagagg gatacgttca agagcgtact atcttcttca aggtaagttt
      721 aaacatatat atactaacta accctgatta tttaaatttt caggatgatg gaaactacaa
      781 gacccgtgct gaggttaagt tcgagggaga tacccttgtt aaccgtatcg agcttaaggg
      841 aatcgatttc aaggaagatg gaaacatcct tggacataag cttgaataca acgctatcca
      901 tggaaacgtt tacatcaccg ctgataagca aaagaacgga attaaggcta acttcggact
      961 caactgtaac atcgaggatg gatccgttca acttgctgat cattaccaac aaaacactcc
     1021 aatcggagat ggaccagttc ttcttccaga taaccactac ctttctaccc aatctaagct
     1081 ttctaaggat ccaaacgaga agcgtgacca catggtcctt cttgagttcg tcaccgctgc
     1141 cggcatcacc cttggaatgg atgagctcta caagtaaggc cgctgtcatc agacatctcg
     1201 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     1261 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     1321 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     1381 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     1441 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     1501 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     1561 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     1621 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     1681 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     1741 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     1801 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     1861 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     1921 tgcccactag tgagtcgtat tacgtagctt ggcgtaatca tggtcatagc tgtttcctgc
     1981 ccgtgaccca gctttcttgt acaaagtggt aagtgcaagt aagatcagtg tttgtttcga
     2041 tatcagtctg tttcgtaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc
     2101 acaactgaga aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta
     2161 aaaaatcgag tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt
     2221 ctcctgggcg tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc
     2281 aattatttca tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca
     2341 tacaaacgag gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct
     2401 aattagctat ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt
     2461 ctaattagaa atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga
     2521 aatgatcccg cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt
     2581 acacaattca agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa
     2641 aaggaggaaa gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag
     2701 gtgagtaacc atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt
     2761 aacagtttcg aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata
     2821 gcctaaacga gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt
     2881 tatcctttca aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt
     2941 tatatcatat ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg
     3001 tagatcgaca acaaggcctc cacttcttca tcgatggcct ctgcagcacc tgcaccacca
     3061 agtttgtatt tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg
     3121 ccagatcgat gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg
     3181 gccacggatt gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac
     3241 aaaatatggt tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag
     3301 atattttggg tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac
     3361 acaaaaagtt cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat
     3421 cgccgtgttc aaaaacacaa ctttattata catagttgat aattcgatgg tatcagctca
     3481 ctcaaagtat agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac
     3541 ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat
     3601 ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa
     3661 caagaatctt tttattgtca gtactgatta gaaaaactca tcgagcatca aatgaaactg
     3721 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     3781 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     3841 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     3901 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat
     3961 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     4021 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     4081 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     4141 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     4201 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     4261 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     4321 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     4381 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     4441 agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct
     4501 cattttatgt ttcctgtgtg aaattgttat ccgctcacaa ttccacagat tatacgagcc
     4561 gatgattaat tgtcaacagc tctttatgtt cggatgtgat gtgagaactg tatcctagca
     4621 agattttaaa aggaagtata tgaaagaaga acctcagtgg caaatcctaa ccttttatat
     4681 ttctctacag gggcgcggcg tggggacaat tcaacgcgtc tgtgagggga gcgtttccct
     4741 gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg cgccgtgcgg ccatcaaaat
     4801 gtatggatgc aaatgattat acatggggat gtatgggcta aatgtacggg cgacagtcac
     4861 atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag ggtattctgg gcctccatgt
     4921 cgggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5041 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccgaaa agtgccacct
     5581 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     5641 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     5701 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     5761 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     5821 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     5881 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     5941 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     6001 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     6061 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     6121 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     6181 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     6241 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     6301 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     6361 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     6421 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     6481 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     6541 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
     6601 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     6661 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     6721 tagtgcgtgt ttatgcttaa atgcgcgcca gggatccgcc aagctatcaa ctttgtatag
     6781 aaaagttgtg tcggattatg ggctcttctt ttatcctcta ctttctttct agaaaatgca
     6841 aatgttgagt cagaaattgc tgaatggagt acaactgcaa tgggtttagc ttatgtttct
     6901 ggaacaatta ctggtggaat tgtgattgaa aagtaaggtt atatttcatt tcaataaatg
     6961 ttaccacagt ataacaaaaa caagttacat tgttcagagt atgtctgaat gtattattat
     7021 tattgtattt gtgcatcagt actgtagttg tactgaagaa gggtttaaaa taatctcaac
     7081 gtttttaaga cgagttagtg agaatacggt cgcagttagg atgtttggac atagttttat
     7141 tagataatcg tgaactaact cactataaaa acccgctaac tcattgaata agatagtttt
     7201 aaacgttcaa aatttgaaga caaaattgta tgaataagtt tacagagttg gtcgacgcaa
     7261 gcttctcctt ctcttcacat ttctcaataa tttagctcta attgcattcg tattcttcgc
     7321 aaaaatacgt attctgattg acccgatgaa atacggatgc ctagttgcat tgatcatcta
     7381 cggttatact tatgggtgag gtttttcaaa tatattttga tttaatcgaa taatgaaatt
     7441 tcagaactgg agttggtcca atttcgtggt ttatatcctc tgaactggta cctcaaaaac
     7501 atcgtagtat tgctcaatct gtggcatatt caatgaatac tgtaatggtg gttatcagta
     7561 catttacagt attacctcta tattccgtta ttggtagtta tgctttcctt attctatatt
     7621 caattccttc atttataagt atgcttatat tatttcgcta cttaccggaa accaaaggac
     7681 gagagattca tgatattgtg aatgaactta aaaaaaaatg aaaacctttg aaaacgttca
     7741 tacggccgaa attttacttg atcgagccaa ttgaactttt gttaagactt acagaaaagt
     7801 gagagatttt gttacaaaaa agtgagaaaa aagcgaaaat gaacaggaaa aagaagaaaa
     7861 atacacccga agtatcatat aaaaattaaa aagattgttt gacctggcgg aacttttttg
     7921 caaaaactct gaatatccat ggcacatgct tcgtgcaaaa caggatccaa atgaatatca
     7981 acaagagatt cttgaagacg acgagccact tgctcagagc attgcttatc tcgaatgaga
     8041 cccttgttaa aatcattttt caaacattat atgacattgt cgaaatgtcc tcctgattcc
     8101 atgatggtag caaactcact tcgtcaagtt tgtacaaaaa agcaggcttg agccaattta
     8161 tccaagtcct tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat
     8221 caaaaacaat ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg
     8281 gctcaaagtt agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc
     8341 ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca
     8401 aattcatcgc ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt
     8461 ctgcacccta tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt
     8521 ctccccagct ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct
     8581 ctctgacgtc ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt
     8641 ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt
     8701 ttttttctgt cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc
     8761 tttttcttct tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac
     8821 cccgcggttc gcaataggtt tctttttcaa attattagcg ttataaatag aaaaatggtg
     8881 gagtttcaat aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt
     8941 tgaaattttt cataccagtt ttccggaaat ccatctgata tcattatcgt atttttcttt
     9001 ttaaaaatgt tttcaaaaaa aaacaaaata tagctggtta tttggcaccc tctaattacc
     9061 attttcctgt cacaccacac tcttttcctt cttccacttc tttccgtttt cagccgcttc
     9121 caaccaaacc gatatgaaag ccgagcaaca acgatcgatt ccacccggct cggcgacctt
     9181 cccgtcgcag gtgagactca gaaaactaga gaaaccgctc aactaactct tgatatccga
     9241 tttcattctt ttctcttttc tttttgttga acttttccat atttccagat gccacggcca
     9301 ccaccaagca ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa agctcaaatc
     9361 actccgaacg acgttttagc acttccggga atcactcaag gtatcttgct ccctttggat
     9421 ttcagtttaa aacataaatt taggattctt atgctcccca tctgcgaaca tctataacat
     9481 cgagttcacc aagttccaaa tccgtgatct ggacactgag caagtgcttt tcgagatcgc
     9541 caaaccggag aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg
     9601 ttatagattt gctccaaact ttttgaaact caaaacggtc ggagcaactg tggagttcaa
     9661 agtaggagac atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg
     9721 ccttctgaag tgttttgact ttgaatttgg attctgtatt ccgaactcac gaaacaactg
     9781 tgaacatatc tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa
     9841 agtacaattc aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga
     9901 gactcgttct gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga
     9961 ctactcatat gatgcataaa tatttaatac aaaaatgttc tggataatta ttctgtcgaa
    10021 tagaaaaaaa actccaaatg tgattaaatt ccaataattc ctgtctagtt tgttccttcc
    10081 ccttcccctt ctcatgttca atgcattcct aagcttttca gttcccccct tgtttctata
    10141 ttttttcgct gtcctgtcac actcgctaaa aacactaatc acacggaaat ctgttttcaa
    10201 taaaaactcc aactttaact cattttcaat ttcaactgca cgggtgtaaa acgacggcca
    10261 gtccgtaata cgactcactt aaggccgcac ctttggtctt ttattgtcaa cttccattgg
    10321 ttcttccatt gtttctgtta aattaatgaa tttttcataa aataaagaca ttatacaata
    10381 taaaaatgaa gaatttattg aaaataaact gccagagaga aaaagtatgc aacactcccg
    10441 ccgagagtgt ttgaaatggt gtacggtaca ttttcgtgct aggagttaga tg
//
