LOCUS       sequence-237691-       12145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR R252C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-R252C
FEATURES             Location/Qualifiers
     source          1..12145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    79..196
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        244..1422
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          475..1413
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1370..1390
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1442..1466
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1469..1540,1541..3403,3404..3472,3473..5101)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCCKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5103..5127)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5180..5221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5242..5809
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5259..5811
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5403..5420)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5630..5649
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5823..5825,5826..5828,5829..6542)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5823..5825,5826..5828,5829..6539)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5868..5889)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6129..6148)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6476..6497
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6556..7144
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6609..6629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7027..7038)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7219..7452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7571..7589)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7657..7761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7762..7830,7831..8622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8793..9381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9282..9301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9535..9552
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9792..9813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9828..9833,9834..9851,9852..9858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9866..9882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9871..9893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9890..9906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9890..9906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9956..9975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10167..10800
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10847..10972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11469..11702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11887..11931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat
       61 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      121 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      181 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      241 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      301 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      361 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      421 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      481 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      541 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      601 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      661 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      721 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      781 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      841 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      901 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      961 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     1021 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1081 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1141 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1201 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1261 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1321 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1381 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccc tgcagatatc
     1441 aacaagtttg tacaaaaaag caggcaccat gcgaccctcc gggacggccg gggcagcgct
     1501 cctggcgctg ctggctgcgc tctgcccggc gagtcgggct ctggaggaaa agaaagtttg
     1561 ccaaggcacg agtaacaagc tcacgcagtt gggcactttt gaagatcatt ttctcagcct
     1621 ccagaggatg ttcaataact gtgaggtggt ccttgggaat ttggaaatta cctatgtgca
     1681 gaggaattat gatctttcct tcttaaagac catccaggag gtggctggtt atgtcctcat
     1741 tgccctcaac acagtggagc gaattccttt ggaaaacctg cagatcatca gaggaaatat
     1801 gtactacgaa aattcctatg ccttagcagt cttatctaac tatgatgcaa ataaaaccgg
     1861 actgaaggag ctgcccatga gaaatttaca ggaaatcctg catggcgccg tgcggttcag
     1921 caacaaccct gccctgtgca acgtggagag catccagtgg cgggacatag tcagcagtga
     1981 ctttctcagc aacatgtcga tggacttcca gaaccacctg ggcagctgcc aaaagtgtga
     2041 tccaagctgt cccaatggga gctgctgggg tgcaggagag gagaactgcc agaaactgac
     2101 caaaatcatc tgtgcccagc agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg
     2161 ctgccacaac cagtgtgctg caggctgcac aggcccccgg gagagcgact gcctggtctg
     2221 ctgcaaattc cgagacgaag ccacgtgcaa ggacacctgc cccccactca tgctctacaa
     2281 ccccaccacg taccagatgg atgtgaaccc cgagggcaaa tacagctttg gtgccacctg
     2341 cgtgaagaag tgtccccgta attatgtggt gacagatcac ggctcgtgcg tccgagcctg
     2401 tggggccgac agctatgaga tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg
     2461 gccttgccgc aaagtgtgta acggaatagg tattggtgaa tttaaagact cactctccat
     2521 aaatgctacg aatattaaac acttcaaaaa ctgcacctcc atcagtggcg atctccacat
     2581 cctgccggtg gcatttaggg gtgactcctt cacacatact cctcctctgg atccacagga
     2641 actggatatt ctgaaaaccg taaaggaaat cacagggttt ttgctgattc aggcttggcc
     2701 tgaaaacagg acggacctcc atgcctttga gaacctagaa atcatacgcg gcaggaccaa
     2761 gcaacatggt cagttttctc ttgcagtcgt cagcctgaac ataacatcct tgggattacg
     2821 ctccctcaag gagataagtg atggagatgt gataatttca ggaaacaaaa atttgtgcta
     2881 tgcaaataca ataaactgga aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat
     2941 aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag gtctgccatg ccttgtgctc
     3001 ccccgagggc tgctggggcc cggagcccag ggactgcgtc tcttgccgga atgtcagccg
     3061 aggcagggaa tgcgtggaca agtgcaacct tctggagggt gagccaaggg agtttgtgga
     3121 gaactctgag tgcatacagt gccacccaga gtgcctgcct caggccatga acatcacctg
     3181 cacaggacgg ggaccagaca actgtatcca gtgtgcccac tacattgacg gcccccactg
     3241 cgtcaagacc tgcccggcag gagtcatggg agaaaacaac accctggtct ggaagtacgc
     3301 agacgccggc catgtgtgcc acctgtgcca tccaaactgc acctacggat gcactgggcc
     3361 aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg tccatcgcca ctgggatggt
     3421 gggggccctc ctcttgctgc tggtggtggc cctggggatc ggcctcttca tgcgaaggcg
     3481 ccacatcgtt cggaagcgca cgctgcggag gctgctgcag gagagggagc ttgtggagcc
     3541 tcttacaccc agtggagaag ctcccaacca agctctcttg aggatcttga aggaaactga
     3601 attcaaaaag atcaaagtgc tgggctccgg tgcgttcggc acggtgtata agggactctg
     3661 gatcccagaa ggtgagaaag ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac
     3721 atctccgaaa gccaacaagg aaatcctcga tgaagcctac gtgatggcca gcgtggacaa
     3781 cccccacgtg tgccgcctgc tgggcatctg cctcacctcc accgtgcagc tcatcacgca
     3841 gctcatgccc ttcggctgcc tcctggacta tgtccgggaa cacaaagaca atattggctc
     3901 ccagtacctg ctcaactggt gtgtgcagat cgcaaagggc atgaactact tggaggaccg
     3961 tcgcttggtg caccgcgacc tggcagccag gaacgtactg gtgaaaacac cgcagcatgt
     4021 caagatcaca gattttgggc tggccaaact gctgggtgcg gaagagaaag aataccatgc
     4081 agaaggaggc aaagtgccta tcaagtggat ggcattggaa tcaattttac acagaatcta
     4141 tacccaccag agtgatgtct ggagctacgg ggtgaccgtt tgggagttga tgacctttgg
     4201 atccaagcca tatgacggaa tccctgccag cgagatctcc tccatcctgg agaaaggaga
     4261 acgcctccct cagccaccca tatgtaccat cgatgtctac atgatcatgg tcaagtgctg
     4321 gatgatagac gcagatagtc gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat
     4381 ggcccgagac ccccagcgct accttgtcat tcagggggat gaaagaatgc atttgccaag
     4441 tcctacagac tccaacttct accgtgccct gatggatgaa gaagacatgg acgacgtggt
     4501 ggatgccgac gagtacctca tcccacagca gggcttcttc agcagcccct ccacgtcacg
     4561 gactcccctc ctgagctctc tgagtgcaac cagcaacaat tccaccgtgg cttgcattga
     4621 tagaaatggg ctgcaaagct gtcccatcaa ggaagacagc ttcttgcagc gatacagctc
     4681 agaccccaca ggcgccttga ctgaggacag catagacgac accttcctcc cagtgcctga
     4741 atacataaac cagtccgttc ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca
     4801 caatcagcct ctgaaccccg cgcccagcag agacccacac taccaggacc cccacagcac
     4861 tgcagtgggc aaccccgagt atctcaacac tgtccagccc acctgtgtca acagcacatt
     4921 cgacagccct gcccactggg cccagaaagg cagccaccaa attagcctgg acaaccctga
     4981 ctaccagcag gacttctttc ccaaggaagc caagccaaat ggcatcttta agggctccac
     5041 agctgaaaat gcagaatacc taagggtcgc gccacaaagc agtgaattta ttggagcata
     5101 gaacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
     5161 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5221 gcgtaccggt tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt
     5281 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5341 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5401 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5461 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5521 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5581 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5641 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5701 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5761 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     5821 atatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5881 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5941 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6001 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     6061 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6121 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6181 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6241 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6301 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6361 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6421 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6481 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6541 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6601 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6661 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6721 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6781 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6841 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6901 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6961 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7021 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7081 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7141 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7201 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7261 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7441 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7501 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     7561 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7621 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7681 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7741 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7801 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7861 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7921 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7981 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     8041 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8101 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8161 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8221 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8281 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8341 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8401 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8461 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8521 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8581 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8641 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8701 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9421 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9481 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9541 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9601 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
     9661 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9721 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
     9781 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     9841 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     9901 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9961 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    10021 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    10081 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    10141 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
    10201 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
    10261 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
    10321 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
    10381 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
    10441 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
    10501 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
    10561 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
    10621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10801 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    10861 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    10921 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10981 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    11041 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    11101 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    11161 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    11221 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    11281 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
    11341 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    11401 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    11461 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    11521 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    11581 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    11641 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    11701 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    11761 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    11821 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    11881 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    11941 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    12001 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    12061 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    12121 atagaagaag aaggtggaga gagag
//
