LOCUS       sequence-237694-       10957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FGFR3 V50I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR3-V50I
FEATURES             Location/Qualifiers
     source          1..10957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    73..190
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        238..1416
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          469..1407
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1364..1384
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1436..1460
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3909..3933)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3986..4027
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4048..4615
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4065..4617
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4209..4226)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4436..4455
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4629..4631,4632..4634,4635..5348)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4629..4631,4632..4634,4635..5345)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4674..4695)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4935..4954)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5282..5303
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5362..5950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5415..5435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5833..5844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6025..6258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6377..6395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6463..6567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6568..6636,6637..7428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6786..6805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7599..8187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8088..8107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8341..8358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8598..8619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8634..8639,8640..8657,8658..8664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8672..8688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8677..8699
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8696..8712
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8696..8712
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8762..8781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8973..9606
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9653..9778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10275..10508
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10693..10737
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt
       61 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      121 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      181 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc
      241 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      301 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      361 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      421 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      481 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      541 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
      601 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
      661 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
      721 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
      781 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
      841 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
      901 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
      961 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     1021 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     1081 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     1141 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     1201 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     1261 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     1321 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     1381 gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag
     1441 tttgtacaaa aaagcagaag ggccgtcaag gcccaccatg ggcgcccctg cctgcgccct
     1501 cgcgctctgc gtggccgtgg ccatcgtggc cggcgcctcc tcggagtcct tggggacgga
     1561 gcagcgcgtc gtggggcgag cggcagaagt cccgggccca gagcccggcc agcaggagca
     1621 gttgatcttc ggcagcgggg atgctgtgga gctgagctgt cccccgcccg ggggtggtcc
     1681 catggggccc actgtctggg tcaaggatgg cacagggctg gtgccctcgg agcgtgtcct
     1741 ggtggggccc cagcggctgc aggtgctgaa tgcctcccac gaggactccg gggcctacag
     1801 ctgccggcag cggctcacgc agcgcgtact gtgccacttc agtgtgcggg tgacagacgc
     1861 tccatcctcg ggagatgacg aagacgggga ggacgaggct gaggacacag gtgtggacac
     1921 aggggcccct tactggacac ggcccgagcg gatggacaag aagctgctgg ccgtgccggc
     1981 cgccaacacc gtccgcttcc gctgcccagc cgctggcaac cccactccct ccatctcctg
     2041 gctgaagaac ggcagggagt tccgcggcga gcaccgcatt ggaggcatca agctgcggca
     2101 tcagcagtgg agcctggtca tggaaagcgt ggtgccctcg gaccgcggca actacacctg
     2161 cgtcgtggag aacaagtttg gcagcatccg gcagacgtac acgctggacg tgctggagcg
     2221 ctccccgcac cggcccatcc tgcaggcggg gctgccggcc aaccagacgg cggtgctggg
     2281 cagcgacgtg gagttccact gcaaggtgta cagtgacgca cagccccaca tccagtggct
     2341 caagcacgtg gaggtgaacg gcagcaaggt gggcccggac ggcacaccct acgttaccgt
     2401 gctcaagacg gcgggcgcta acaccaccga caaggagcta gaggttctct ccttgcacaa
     2461 cgtcaccttt gaggacgccg gggagtacac ctgcctggcg ggcaattcta ttgggttttc
     2521 tcatcactct gcgtggctgg tggtgctgcc agccgaggag gagctggtgg aggctgacga
     2581 ggcgggcagt gtgtatgcag gcatcctcag ctacggggtg ggcttcttcc tgttcatcct
     2641 ggtggtggcg gctgtgacgc tctgccgcct gcgcagcccc cccaagaaag gcctgggctc
     2701 ccccaccgtg cacaagatct cccgcttccc gctcaagcga caggtgtccc tggagtccaa
     2761 cgcgtccatg agctccaaca caccactggt gcgcatcgca aggctgtcct caggggaggg
     2821 ccccacgctg gccaatgtct ccgagctcga gctgcctgcc gaccccaaat gggagctgtc
     2881 tcgggcccgg ctgaccctgg gcaagcccct tggggagggc tgcttcggcc aggtggtcat
     2941 ggcggaggcc atcggcattg acaaggaccg ggccgccaag cctgtcaccg tagccgtgaa
     3001 gatgctgaaa gacgatgcca ctgacaagga cctgtcggac ctggtgtctg agatggagat
     3061 gatgaagatg atcgggaaac acaaaaacat catcaacctg ctgggcgcct gcacgcaggg
     3121 cgggcccctg tacgtgctgg tggagtacgc ggccaagggt aacctgcggg agtttctgcg
     3181 ggcgcggcgg cccccgggcc tggactactc cttcgacacc tgcaagccgc ccgaggagca
     3241 gctcaccttc aaggacctgg tgtcctgtgc ctaccaggtg gcccggggca tggagtactt
     3301 ggcctcccag aagtgcatcc acagggacct ggctgcccgc aatgtgctgg tgaccgagga
     3361 caacgtgatg aagatcgcag acttcgggct ggcccgggac gtgcacaacc tcgactacta
     3421 caagaagaca accaacggcc ggctgcccgt gaagtggatg gcgcctgagg ccttgtttga
     3481 ccgagtctac actcaccaga gtgacgtctg gtcctttggg gtcctgctct gggagatctt
     3541 cacgctgggg ggctccccgt accccggcat ccctgtggag gagctcttca agctgctgaa
     3601 ggagggccac cgcatggaca agcccgccaa ctgcacacac gacctgtaca tgatcatgcg
     3661 ggagtgctgg catgccgcgc cctcccagag gcccaccttc aagcagctgg tggaggacct
     3721 ggaccgtgtc cttaccgtga cgtccaccga cgagtacctg gacctgtcgg cgcctttcga
     3781 gcagtactcc ccgggtggcc aggacacccc cagctccagc tcctcagggg acgactccgt
     3841 gtttgcccac gacctgctgc ccccggcccc acccagcagt gggggctcgc ggacgtaggg
     3901 cctcatgggc ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
     3961 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     4021 ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag
     4081 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4141 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4201 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4261 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4321 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4381 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4441 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4501 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4561 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4621 ccacacatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     4681 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4741 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4801 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4861 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4921 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4981 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     5041 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     5101 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     5161 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     5221 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     5281 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     5341 acaagtaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     5401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5761 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5881 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5941 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6001 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6241 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6301 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     6361 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6421 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6481 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6541 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6601 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6661 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6721 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6781 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6841 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6901 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6961 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7021 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7081 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7141 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7201 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7261 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7321 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7381 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7441 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7501 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7561 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7621 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7681 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7741 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7801 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7861 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7921 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7981 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8041 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8101 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8161 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8221 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8281 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8341 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8401 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     8461 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8521 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     8581 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8641 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8701 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     8761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     8821 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     8881 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     8941 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     9001 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     9061 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     9121 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     9181 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     9241 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     9301 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     9361 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     9421 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9481 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9541 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9601 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     9661 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9721 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     9781 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     9841 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9901 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9961 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
    10021 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
    10081 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
    10141 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    10201 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    10261 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    10321 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    10381 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    10441 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    10501 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    10561 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    10621 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    10681 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    10741 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    10801 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    10861 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    10921 gaaggaatag aagaagaagg tggagagaga gacagag
//
