LOCUS       sequence-237695-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mNeptune under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP608
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1533..1549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1533..1549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1556..1580)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3008..3028)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3056..3252)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3258..4073)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3258..4073)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3981..4000
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4083..4105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4094..4110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4119..4124,4125..4140,4141..4146))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      4501..5089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4990..5009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5152..6315
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6338..6358
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7694..7718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7864..7875)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9815..9832
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9816..9832
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9857..10456
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 ctttgctcta gaatggtttc taagggagag gagcttatca aggagaacat gcacatgaag
       61 ctctacatgg agggaaccgt caacaaccac cacttcaagt gcacctccga gggagaggga
      121 aagccatacg agggaaccca aaccggacgt atcaaggtcg tcgagggagg accactccca
      181 ttcgccttcg acatcctcgc cacctgcttc atgtacggat ctaagacctt catcaaccac
      241 acccaaggaa tcccagactt cttcaagcaa tccttcccag agggattcac ctgggagcgt
      301 gtcaccacct acgaggacgg aggagtcctc accgccaccc aagacacctc cctccaagac
      361 ggatgcctca tctacaacgt caagatccgt ggagtcaact tcccatccaa cggaccagtc
      421 atgcaaaaga agaccctcgg atgggaggcc tccaccgaga ccctctaccc agccgacgga
      481 ggacttgagg gacgttgcga catggccctc aagctcgtcg gaggaggaca cctcatctgc
      541 aacctcaaga ccacctaccg ttccaagaag ccagccaaga acctcaagat gccaggagtc
      601 tacttcgtcg accgtcgtct tgagcgtatc aaggaggccg acaacgagac ctacgtcgag
      661 caacacgagg tcgccgtcgc ccgttactgc gacctcccat ccaagctcgg acacaagctc
      721 aacggaatgg acgagcttta caagtaaggc cgctgtcatc agacatctcg cgcccgtgcc
      781 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
      841 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
      901 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
      961 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     1021 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     1081 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     1141 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaagag
     1201 ttttggagta tttttgaaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     1261 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     1321 ctaaaatcac aagtattgat gagcacgatg caagaaagat cgggagaagg tttgggtttg
     1381 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     1441 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tgcccactag
     1501 tgagtcgtat tacgtagctt ggcgtaatca tggtcatagc tgtttcctgc ccgtgaccca
     1561 gctttcttgt acaaagtggt aagtgcaagt aagatcagtg tttgtttcga tatcagtctg
     1621 tttcgtaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc acaactgaga
     1681 aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag
     1741 tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg
     1801 tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc aattatttca
     1861 tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca tacaaacgag
     1921 gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct aattagctat
     1981 ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt ctaattagaa
     2041 atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga aatgatcccg
     2101 cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt acacaattca
     2161 agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa aaggaggaaa
     2221 gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag gtgagtaacc
     2281 atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt aacagtttcg
     2341 aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata gcctaaacga
     2401 gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt tatcctttca
     2461 aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt tatatcatat
     2521 ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg tagatcgaca
     2581 acaaggcctc cacttcttca tcgatggcct ctgcagcacc tgcaccacca agtttgtatt
     2641 tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg ccagatcgat
     2701 gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg gccacggatt
     2761 gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac aaaatatggt
     2821 tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag atattttggg
     2881 tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac acaaaaagtt
     2941 cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat cgccgtgttc
     3001 aaaaacacaa ctttattata catagttgat aattcgatgg tatcagctca ctcaaagtat
     3061 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
     3121 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
     3181 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
     3241 tttattgtca gtactgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     3301 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     3361 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     3421 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     3481 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     3541 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     3601 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     3661 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     3721 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     3781 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     3841 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     3901 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     3961 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     4021 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cattttatgt
     4081 ttcctgtgtg aaattgttat ccgctcacaa ttccacagat tatacgagcc gatgattaat
     4141 tgtcaacagc tctttatgtt cggatgtgat gtgagaactg tatcctagca agattttaaa
     4201 aggaagtata tgaaagaaga acctcagtgg caaatcctaa ccttttatat ttctctacag
     4261 gggcgcggcg tggggacaat tcaacgcgtc tgtgagggga gcgtttccct gctcgcaggt
     4321 ctgcagcgag gagccgtaat ttttgcttcg cgccgtgcgg ccatcaaaat gtatggatgc
     4381 aaatgattat acatggggat gtatgggcta aatgtacggg cgacagtcac atcatgcccc
     4441 tgagctgcgc acgtcaagac tgtcaaggag ggtattctgg gcctccatgt cgggatcttc
     4501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4621 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5101 cgcggccttt ttacggttcc tggccttttg ctggccgaaa agtgccacct gaacgaagca
     5161 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     5221 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     5281 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     5341 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     5401 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     5461 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     5521 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     5581 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     5641 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     5701 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     5761 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     5821 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     5881 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     5941 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     6001 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     6061 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     6121 cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     6181 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     6241 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     6301 ttatgcttaa atgcgcgcca gggatccgcc aagctatcaa ctttgtatag aaaagttgtg
     6361 tcggattatg ggctcttctt ttatcctcta ctttctttct agaaaatgca aatgttgagt
     6421 cagaaattgc tgaatggagt acaactgcaa tgggtttagc ttatgtttct ggaacaatta
     6481 ctggtggaat tgtgattgaa aagtaaggtt atatttcatt tcaataaatg ttaccacagt
     6541 ataacaaaaa caagttacat tgttcagagt atgtctgaat gtattattat tattgtattt
     6601 gtgcatcagt actgtagttg tactgaagaa gggtttaaaa taatctcaac gtttttaaga
     6661 cgagttagtg agaatacggt cgcagttagg atgtttggac atagttttat tagataatcg
     6721 tgaactaact cactataaaa acccgctaac tcattgaata agatagtttt aaacgttcaa
     6781 aatttgaaga caaaattgta tgaataagtt tacagagttg gtcgacgcaa gcttctcctt
     6841 ctcttcacat ttctcaataa tttagctcta attgcattcg tattcttcgc aaaaatacgt
     6901 attctgattg acccgatgaa atacggatgc ctagttgcat tgatcatcta cggttatact
     6961 tatgggtgag gtttttcaaa tatattttga tttaatcgaa taatgaaatt tcagaactgg
     7021 agttggtcca atttcgtggt ttatatcctc tgaactggta cctcaaaaac atcgtagtat
     7081 tgctcaatct gtggcatatt caatgaatac tgtaatggtg gttatcagta catttacagt
     7141 attacctcta tattccgtta ttggtagtta tgctttcctt attctatatt caattccttc
     7201 atttataagt atgcttatat tatttcgcta cttaccggaa accaaaggac gagagattca
     7261 tgatattgtg aatgaactta aaaaaaaatg aaaacctttg aaaacgttca tacggccgaa
     7321 attttacttg atcgagccaa ttgaactttt gttaagactt acagaaaagt gagagatttt
     7381 gttacaaaaa agtgagaaaa aagcgaaaat gaacaggaaa aagaagaaaa atacacccga
     7441 agtatcatat aaaaattaaa aagattgttt gacctggcgg aacttttttg caaaaactct
     7501 gaatatccat ggcacatgct tcgtgcaaaa caggatccaa atgaatatca acaagagatt
     7561 cttgaagacg acgagccact tgctcagagc attgcttatc tcgaatgaga cccttgttaa
     7621 aatcattttt caaacattat atgacattgt cgaaatgtcc tcctgattcc atgatggtag
     7681 caaactcact tcgtcaagtt tgtacaaaaa agcaggcttg agccaattta tccaagtcct
     7741 tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat caaaaacaat
     7801 ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg gctcaaagtt
     7861 agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc ccaacaaggg
     7921 atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca aattcatcgc
     7981 ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt ctgcacccta
     8041 tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt ctccccagct
     8101 ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct ctctgacgtc
     8161 ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt ttcccatttc
     8221 tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt ttttttctgt
     8281 cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc tttttcttct
     8341 tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac cccgcggttc
     8401 gcaataggtt tctttttcaa attattagcg ttataaatag aaaaatggtg gagtttcaat
     8461 aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt tgaaattttt
     8521 cataccagtt ttccggaaat ccatctgata tcattatcgt atttttcttt ttaaaaatgt
     8581 tttcaaaaaa aaacaaaata tagctggtta tttggcaccc tctaattacc attttcctgt
     8641 cacaccacac tcttttcctt cttccacttc tttccgtttt cagccgcttc caaccaaacc
     8701 gatatgaaag ccgagcaaca acgatcgatt ccacccggct cggcgacctt cccgtcgcag
     8761 gtgagactca gaaaactaga gaaaccgctc aactaactct tgatatccga tttcattctt
     8821 ttctcttttc tttttgttga acttttccat atttccagat gccacggcca ccaccaagca
     8881 ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa agctcaaatc actccgaacg
     8941 acgttttagc acttccggga atcactcaag gtatcttgct ccctttggat ttcagtttaa
     9001 aacataaatt taggattctt atgctcccca tctgcgaaca tctataacat cgagttcacc
     9061 aagttccaaa tccgtgatct ggacactgag caagtgcttt tcgagatcgc caaaccggag
     9121 aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg ttatagattt
     9181 gctccaaact ttttgaaact caaaacggtc ggagcaactg tggagttcaa agtaggagac
     9241 atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg ccttctgaag
     9301 tgttttgact ttgaatttgg attctgtatt ccgaactcac gaaacaactg tgaacatatc
     9361 tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa agtacaattc
     9421 aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga gactcgttct
     9481 gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga ctactcatat
     9541 gatgcataaa tatttaatac aaaaatgttc tggataatta ttctgtcgaa tagaaaaaaa
     9601 actccaaatg tgattaaatt ccaataattc ctgtctagtt tgttccttcc ccttcccctt
     9661 ctcatgttca atgcattcct aagcttttca gttcccccct tgtttctata ttttttcgct
     9721 gtcctgtcac actcgctaaa aacactaatc acacggaaat ctgttttcaa taaaaactcc
     9781 aactttaact cattttcaat ttcaactgca cgggtgtaaa acgacggcca gtccgtaata
     9841 cgactcactt aaggccgcac ctttggtctt ttattgtcaa cttccattgg ttctttcatt
     9901 gtttctgtta aattaatgaa tttttcataa aataaagaca ttatacaata taaaaatgaa
     9961 gaatttattg aaaataaact gccagagaga aaaagtatgc aacactcccg ccgagagtgt
    10021 ttgaaatggt gtacggtaca ttttcgtgct aggagttaga tgtgcaggca gcaacgagag
    10081 ggggagagat ttttttgggc cttgtgaaat taacgtgagt tttctggtca tctgactaat
    10141 catgttggtt ttttgttggt ttattttgtt tttatctttg tttttatcca gattaggaaa
    10201 tttaaatttt atgaatttat aatgaggtca aacattcagt cccagcgttt ttcctgttct
    10261 cactgtttag tcgaattttt attttaggct ttcaacaaat gttctaactg tcttatttgt
    10321 gacctcactt tttatatttt tttaattttt aaaaatatta gaagtttcta ggataatttt
    10381 ttcgactttt attctctcta ccgtccgcac tcttcttact tttaaattaa attgtttttt
    10441 tttcagttgg gaaaca
//
