LOCUS       sequence-237696-       10435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mtagBFP2 under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP610
FEATURES             Location/Qualifiers
     source          1..10435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1512..1528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1512..1528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1535..1559)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2987..3007)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3035..3231)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3237..4052)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3237..4052)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3960..3979
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4062..4084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4073..4089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4098..4103,4104..4119,4120..4125))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      4480..5068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4969..4988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5131..6294
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6317..6337
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7673..7697
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7843..7854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9794..9811
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9795..9811
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9836..10435
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 ctttgctcta gaatggtctc caagggagag gagcttatca aggagaacat gcacatgaag
       61 ctctacatgg agggaaccgt tgacaaccac cacttcaagt gcacctccga gggagaggga
      121 aagccatacg agggaaccca aaccatgcgt atcaaggtcg tcgagggagg accactccca
      181 ttcgccttcg acatcctcgc cacctccttc ctctacggtt ccaagacctt catcaaccac
      241 acccaaggaa tcccagactt cttcaagcaa tccttcccag agggattcac ctgggagcgt
      301 gtcaccacct acgaggacgg aggagtcctc accgccaccc aagacacctc cctccaagac
      361 ggatgcctca tctacaacgt caagatccgt ggagtcaact tcacctccaa cggaccagtc
      421 atgcaaaaga agaccctcgg atgggaggcc ttcaccgaga ccctctaccc agccgacgga
      481 ggacttgagg gacgtaacga catggccctc aagctcgtcg gaggatctca cctcatcgcc
      541 aacgccaaga ccacctaccg ttccaagaag ccagccaaga acctcaagat gccaggagtc
      601 tactacgtcg attaccgtct tgagcgtatc aaggaggcca acaacgagac ctacgtcgag
      661 caacacgagg tcgccgtcgc ccgttactgc gacctcccat ccaagctcgg acacaagctc
      721 aactaaggcc gctgtcatca gacatctcgc gcccgtgcct ctgacttcta agtccaatta
      781 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
      841 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
      901 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
      961 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     1021 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     1081 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg
     1141 ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt
     1201 tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc
     1261 ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg
     1321 agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta
     1381 gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat
     1441 acattcccaa tataccaaac ataactgttt gcccactagt gagtcgtatt acgtagcttg
     1501 gcgtaatcat ggtcatagct gtttcctgcc cgtgacccag ctttcttgta caaagtggta
     1561 agtgcaagta agatcagtgt ttgtttcgat atcagtctgt ttcgtaacgg tcttctgtat
     1621 aactacaaaa aagaataaaa aaccgtatca caactgagaa aaaagacaaa acttattgaa
     1681 cagtgttcct ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac
     1741 gcaatcaaaa acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta
     1801 ccattattgt ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt
     1861 cttgcattta ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag
     1921 tcacctctaa ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac
     1981 cgtaattaga aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa
     2041 tatggcattt tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac
     2101 aagccaaatt caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa
     2161 caatggcgaa gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa
     2221 gacttcttga aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta
     2281 agggctatag atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg
     2341 tgcgaaggaa tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt
     2401 tattttttca aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag
     2461 atcatatcca tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa
     2521 ggaaaatctc aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcat
     2581 cgatggcctc tgcagcacct gcaccaccaa gtttgtattt ttcatattaa tataaagtga
     2641 gccacgcata gtcgttttta ggatttttgc cagatcgatg tcgcgcaaac agactgtttt
     2701 tttttctttg aaagccgtgc gtgacatggg ccacggattg taacgccgcg ctggatggat
     2761 aaaaatttat tcagtttttt agcctttaca aaatatggtt cgactacttt ctttattttt
     2821 acatttttat aggtgctcat tgagctgaga tattttgggt ataatcggaa aatcgctgat
     2881 tttggtcatt tttctgcact aaagattaca caaaaagttc agtatttaga ttcattgttg
     2941 aaaattgggg atgatctaca gatacatatc gccgtgttca aaaacacaac tttattatac
     3001 atagttgata attcgatggt atcagctcac tcaaagtata gcgaccagca ttcacatacg
     3061 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
     3121 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
     3181 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattag
     3241 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     3301 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     3361 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     3421 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     3481 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     3541 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     3601 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     3661 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     3721 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     3781 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     3841 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     3901 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     3961 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     4021 gagcaagacg tttcccgttg aatatggctc attttatgtt tcctgtgtga aattgttatc
     4081 cgctcacaat tccacagatt atacgagccg atgattaatt gtcaacagct ctttatgttc
     4141 ggatgtgatg tgagaactgt atcctagcaa gattttaaaa ggaagtatat gaaagaagaa
     4201 cctcagtggc aaatcctaac cttttatatt tctctacagg ggcgcggcgt ggggacaatt
     4261 caacgcgtct gtgaggggag cgtttccctg ctcgcaggtc tgcagcgagg agccgtaatt
     4321 tttgcttcgc gccgtgcggc catcaaaatg tatggatgca aatgattata catggggatg
     4381 tatgggctaa atgtacgggc gacagtcaca tcatgcccct gagctgcgca cgtcaagact
     4441 gtcaaggagg gtattctggg cctccatgtc gggatcttct tgagatcctt tttttctgcg
     4501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4621 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5101 ggccttttgc tggccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa
     5161 caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca
     5221 gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg
     5281 taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     5341 ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt
     5401 ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta
     5461 ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg
     5521 tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct
     5581 gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata
     5641 aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag
     5701 tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc
     5761 tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga
     5821 atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg
     5881 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg
     5941 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta
     6001 ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt
     6061 cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagctagaga
     6121 ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc
     6181 aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct
     6241 gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgcgccag
     6301 ggatccgcca agctatcaac tttgtataga aaagttgtgt cggattatgg gctcttcttt
     6361 tatcctctac tttctttcta gaaaatgcaa atgttgagtc agaaattgct gaatggagta
     6421 caactgcaat gggtttagct tatgtttctg gaacaattac tggtggaatt gtgattgaaa
     6481 agtaaggtta tatttcattt caataaatgt taccacagta taacaaaaac aagttacatt
     6541 gttcagagta tgtctgaatg tattattatt attgtatttg tgcatcagta ctgtagttgt
     6601 actgaagaag ggtttaaaat aatctcaacg tttttaagac gagttagtga gaatacggtc
     6661 gcagttagga tgtttggaca tagttttatt agataatcgt gaactaactc actataaaaa
     6721 cccgctaact cattgaataa gatagtttta aacgttcaaa atttgaagac aaaattgtat
     6781 gaataagttt acagagttgg tcgacgcaag cttctccttc tcttcacatt tctcaataat
     6841 ttagctctaa ttgcattcgt attcttcgca aaaatacgta ttctgattga cccgatgaaa
     6901 tacggatgcc tagttgcatt gatcatctac ggttatactt atgggtgagg tttttcaaat
     6961 atattttgat ttaatcgaat aatgaaattt cagaactgga gttggtccaa tttcgtggtt
     7021 tatatcctct gaactggtac ctcaaaaaca tcgtagtatt gctcaatctg tggcatattc
     7081 aatgaatact gtaatggtgg ttatcagtac atttacagta ttacctctat attccgttat
     7141 tggtagttat gctttcctta ttctatattc aattccttca tttataagta tgcttatatt
     7201 atttcgctac ttaccggaaa ccaaaggacg agagattcat gatattgtga atgaacttaa
     7261 aaaaaaatga aaacctttga aaacgttcat acggccgaaa ttttacttga tcgagccaat
     7321 tgaacttttg ttaagactta cagaaaagtg agagattttg ttacaaaaaa gtgagaaaaa
     7381 agcgaaaatg aacaggaaaa agaagaaaaa tacacccgaa gtatcatata aaaattaaaa
     7441 agattgtttg acctggcgga acttttttgc aaaaactctg aatatccatg gcacatgctt
     7501 cgtgcaaaac aggatccaaa tgaatatcaa caagagattc ttgaagacga cgagccactt
     7561 gctcagagca ttgcttatct cgaatgagac ccttgttaaa atcatttttc aaacattata
     7621 tgacattgtc gaaatgtcct cctgattcca tgatggtagc aaactcactt cgtcaagttt
     7681 gtacaaaaaa gcaggcttga gccaatttat ccaagtcctt gtaaaaagta ttcgaaaatt
     7741 gttaacggat aaaatgttta ttataatatc aaaaacaatt tgtcagttga ccactttttg
     7801 atatatttga cagaaaccgg gatgaattgg ctcaaagtta gggcgccctt ctattacagg
     7861 gtttctgata acaaaccggt tattaactcc caacaaggga tgatttcaat tcatcatgct
     7921 caaattgacc caaattaagt tacatgacaa attcatcgcc cttttcaact cttcttggct
     7981 catcatctgt tattctgttc ctattctgtc tgcaccctat acccctttgc atactctctc
     8041 gtcattcctt tcttggatag tgtcttcttc tccccagctc tgctacttct atgacttgcg
     8101 cccgctgctt ttccgctcgt tcctctcctc tctgacgtct tcttctgctt cttttctcct
     8161 tcctttttcc cttctcaatc ttcctctttt tcccatttct gtcaatcatt cgaagaagaa
     8221 gaagaagaag accctcattc atttcatttt tttttctgtc ggtgtgtgct gctcggttaa
     8281 gagtgagctc tctattccac gtcttcttct ttttcttctt cgattcgaat caatcactcc
     8341 accaaaacgc attcgttttt gggattcacc ccgcggttcg caataggttt ctttttcaaa
     8401 ttattagcgt tataaataga aaaatggtgg agtttcaata aaaatgataa tttacaaagt
     8461 gtattttgat tacatgtact caaaaaggtt gaaatttttc ataccagttt tccggaaatc
     8521 catctgatat cattatcgta tttttctttt taaaaatgtt ttcaaaaaaa aacaaaatat
     8581 agctggttat ttggcaccct ctaattacca ttttcctgtc acaccacact cttttccttc
     8641 ttccacttct ttccgttttc agccgcttcc aaccaaaccg atatgaaagc cgagcaacaa
     8701 cgatcgattc cacccggctc ggcgaccttc ccgtcgcagg tgagactcag aaaactagag
     8761 aaaccgctca actaactctt gatatccgat ttcattcttt tctcttttct ttttgttgaa
     8821 cttttccata tttccagatg ccacggccac caccaagcac cgaacaagga atcacaacgg
     8881 aatcggagct tgcgaagaaa gctcaaatca ctccgaacga cgttttagca cttccgggaa
     8941 tcactcaagg tatcttgctc cctttggatt tcagtttaaa acataaattt aggattctta
     9001 tgctccccat ctgcgaacat ctataacatc gagttcacca agttccaaat ccgtgatctg
     9061 gacactgagc aagtgctttt cgagatcgcc aaaccggaga acgatcagga gaatgatgag
     9121 tcgccacagg agtcggcaag atacgtgcgt tatagatttg ctccaaactt tttgaaactc
     9181 aaaacggtcg gagcaactgt ggagttcaaa gtaggagaca tcccaatcca tcatttccga
     9241 atgatcgaac gtcacttctt caaagatcgc cttctgaagt gttttgactt tgaatttgga
     9301 ttctgtattc cgaactcacg aaacaactgt gaacatatct atgagttccc tcaactctct
     9361 caacaactca gtgagtcatt attctaaaaa gtacaattca aaagactaat ctctttttca
     9421 gtggacgaca tgatcaacaa tccaaacgag actcgttctg acagcttcta tttcgtcgat
     9481 aacaaactcg tcatgcacaa caaagccgac tactcatatg atgcataaat atttaataca
     9541 aaaatgttct ggataattat tctgtcgaat agaaaaaaaa ctccaaatgt gattaaattc
     9601 caataattcc tgtctagttt gttccttccc cttccccttc tcatgttcaa tgcattccta
     9661 agcttttcag ttcccccctt gtttctatat tttttcgctg tcctgtcaca ctcgctaaaa
     9721 acactaatca cacggaaatc tgttttcaat aaaaactcca actttaactc attttcaatt
     9781 tcaactgcac gggtgtaaaa cgacggccag tccgtaatac gactcactta aggccgcacc
     9841 tttggtcttt tattgtcaac ttccattggt tcttccattg tttctgttaa attaatgaat
     9901 ttttcataaa ataaagacat tatacaatat aaaaatgaag aatttattga aaataaactg
     9961 ccagagagaa aaagtatgca acactcccgc cgagagtgtt tgaaatggtg tacggtacat
    10021 tttcgtgcta ggagttagat gtgcaggcag caacgagagg gggagagatt tttttgggcc
    10081 ttgtgaaatt aacgtgagtt ttctggtcat ctgactaatc atgttggttt tttgttggtt
    10141 tattttgttt ttatctttgt ttttatccag attaggaaat ttaaatttta tgaatttata
    10201 atgaggtcaa acattcagtc ccagcgtttt tcctgttctc actgtttagt cgaattttta
    10261 ttttaggctt tcaacaaatg ttctaactgt cttatttgtg acctcacttt ttatattttt
    10321 ttaattttta aaaatattag aagtttctag gataattttt tcgactttta ttctctctac
    10381 cgtccgcact cttcttactt ttaaattaaa ttgttttttt ttcagttggg aaaca
//
