LOCUS       sequence-237697-       12280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB2 G776V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB2-G776V
FEATURES             Location/Qualifiers
     source          1..12280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    99..216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        264..1442
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          495..1433
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1390..1410
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1462..1486
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5258..5282)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5335..5376
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5397..5964
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5414..5966
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5558..5575)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5785..5804
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5978..5980,5981..5983,5984..6697)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5978..5980,5981..5983,5984..6694)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6023..6044)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6284..6303)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6631..6652
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6711..7299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6764..6784)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7182..7193)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7374..7607
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7726..7744)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7812..7916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7917..7985,7986..8777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8135..8154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8948..9536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9437..9456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9690..9707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9947..9968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9983..9988,9989..10006,10007..10013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10021..10037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10026..10048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10045..10061
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10045..10061
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10111..10130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10322..10955
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11002..11127
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11624..11857
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12042..12086
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
       61 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
      121 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      181 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      241 gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
      301 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
      361 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      421 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      481 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
      541 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
      601 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
      661 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
      721 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
      781 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
      841 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
      901 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
      961 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     1021 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     1081 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     1141 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     1201 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     1261 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     1321 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     1381 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     1441 gaagcggccc tgcagatatc aacaagtttg tacaaaaaag caggcaccat ggagctggcg
     1501 gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc ccggagccgc gagcacccaa
     1561 gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca gtcccgagac ccacctggac
     1621 atgctccgcc acctctacca gggctgccag gtggtgcagg gaaacctgga actcacctac
     1681 ctgcccacca atgccagcct gtccttcctg caggatatcc aggaggtgca gggctacgtg
     1741 ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga ggctgcggat tgtgcgaggc
     1801 acccagctct ttgaggacaa ctatgccctg gccgtgctag acaatggaga cccgctgaac
     1861 aataccaccc ctgtcacagg ggcctcccca ggaggcctgc gggagctgca gcttcgaagc
     1921 ctcacagaga tcttgaaagg aggggtcttg atccagcgga acccccagct ctgctaccag
     1981 gacacgattt tgtggaagga catcttccac aagaacaacc agctggctct cacactgata
     2041 gacaccaacc gctctcgggc ctgccacccc tgttctccga tgtgtaaggg ctcccgctgc
     2101 tggggagaga gttctgagga ttgtcagagc ctgacgcgca ctgtctgtgc cggtggctgt
     2161 gcccgctgca aggggccact gcccactgac tgctgccatg agcagtgtgc tgccggctgc
     2221 acgggcccca agcactctga ctgcctggcc tgcctccact tcaaccacag tggcatctgt
     2281 gagctgcact gcccagccct ggtcacctac aacacagaca cgtttgagtc catgcccaat
     2341 cccgagggcc ggtatacatt cggcgccagc tgtgtgactg cctgtcccta caactacctt
     2401 tctacggacg tgggatcctg caccctcgtc tgccccctgc acaaccaaga ggtgacagca
     2461 gaggatggaa cacagcggtg tgagaagtgc agcaagccct gtgcccgagt gtgctatggt
     2521 ctgggcatgg agcacttgcg agaggtgagg gcagttacca gtgccaatat ccaggagttt
     2581 gctggctgca agaagatctt tgggagcctg gcatttctgc cggagagctt tgatggggac
     2641 ccagcctcca acactgcccc gctccagcca gagcagctcc aagtgtttga gactctggaa
     2701 gagatcacag gttacctata catctcagca tggccggaca gcctgcctga cctcagcgtc
     2761 ttccagaacc tgcaagtaat ccggggacga attctgcaca atggcgccta ctcgctgacc
     2821 ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac tgagggaact gggcagtgga
     2881 ctggccctca tccaccataa cacccacctc tgcttcgtgc acacggtgcc ctgggaccag
     2941 ctctttcgga acccgcacca agctctgctc cacactgcca accggccaga ggacgagtgt
     3001 gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag ggcactgctg gggtccaggg
     3061 cccacccagt gtgtcaactg cagccagttc cttcggggcc aggagtgcgt ggaggaatgc
     3121 cgagtactgc aggggctccc cagggagtat gtgaatgcca ggcactgttt gccgtgccac
     3181 cctgagtgtc agccccagaa tggctcagtg acctgttttg gaccggaggc tgaccagtgt
     3241 gtggcctgtg cccactataa ggaccctccc ttctgcgtgg cccgctgccc cagcggtgtg
     3301 aaacctgacc tctcctacat gcccatctgg aagtttccag atgaggaggg cgcatgccag
     3361 ccttgcccca tcaactgcac ccactcctgt gtggacctgg atgacaaggg ctgccccgcc
     3421 gagcagagag ccagccctct gacgtccatc atctctgcgg tggttggcat tctgctggtc
     3481 gtggtcttgg gggtggtctt tgggatcctc atcaagcgac ggcagcagaa gatccggaag
     3541 tacacgatgc ggagactgct gcaggaaacg gagctggtgg agccgctgac acctagcgga
     3601 gcgatgccca accaggcgca gatgcggatc ctgaaagaga cggagctgag gaaggtgaag
     3661 gtgcttggat ctggcgcttt tggcacagtc tacaagggca tctggatccc tgatggggag
     3721 aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa acacatcccc caaagccaac
     3781 aaagaaatct tagacgaagc atacgtgatg gctgttgtgg gctccccata tgtctcccgc
     3841 cttctgggca tctgcctgac atccacggtg cagctggtga cacagcttat gccctatggc
     3901 tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg gctcccagga cctgctgaac
     3961 tggtgtatgc agattgccaa ggggatgagc tacctggagg atgtgcggct cgtacacagg
     4021 gacttggccg ctcggaacgt gctggtcaag agtcccaacc atgtcaaaat tacagacttc
     4081 gggctggctc ggctgctgga cattgacgag acagagtacc atgcagatgg gggcaaggtg
     4141 cccatcaagt ggatggcgct ggagtccatt ctccgccggc ggttcaccca ccagagtgat
     4201 gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt ttggggccaa accttacgat
     4261 gggatcccag cccgggagat ccctgacctg ctggaaaagg gggagcggct gccccagccc
     4321 cccatctgca ccattgatgt ctacatgatc atggtcaaat gttggatgat tgactctgaa
     4381 tgtcggccaa gattccggga gttggtgtct gaattctccc gcatggccag ggacccccag
     4441 cgctttgtgg tcatccagaa tgaggacttg ggcccagcca gtcccttgga cagcaccttc
     4501 taccgctcac tgctggagga cgatgacatg ggggacctgg tggatgctga ggagtatctg
     4561 gtaccccagc agggcttctt ctgtccagac cctgccccgg gcgctggggg catggtccac
     4621 cacaggcacc gcagctcatc taccaggagt ggcggtgggg acctgacact agggctggag
     4681 ccctctgaag aggaggcccc caggtctcca ctggcaccct ccgaaggggc tggctccgat
     4741 gtatttgatg gtgacctggg aatgggggca gccaaggggc tgcaaagcct ccccacacat
     4801 gaccccagcc ctctacagcg gtacagtgag gaccccacag tacccctgcc ctctgagact
     4861 gatggctacg ttgcccccct gacctgcagc ccccagcctg aatatgtgaa ccagccagat
     4921 gttcggcccc agcccccttc gccccgagag ggccctctgc ctgctgcccg acctgctggt
     4981 gccactctgg aaaggcccaa gactctctcc ccagggaaga atggggtcgt caaagacgtt
     5041 tttgcctttg ggggtgccgt ggagaacccc gagtacttga caccccaggg aggagctgcc
     5101 cctcagcccc accctcctcc tgccttcagc ccagccttcg acaacctcta ttactgggac
     5161 caggacccac cagagcgggg ggctccaccc agcaccttca aagggacacc tacggcagag
     5221 aacccagagt acctgggtct ggacgtgcca gtgtagaacc cagctttctt gtacaaagtg
     5281 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     5341 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     5401 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5461 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5521 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5581 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5641 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5701 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5761 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5821 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5881 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5941 ttcctttgaa aaacacgatg ataatatggc cacacatatg gtgagcaagg gcgaggagct
     6001 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     6061 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6121 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     6181 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6241 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6301 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6361 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6421 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6481 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6541 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6601 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6661 cgggatcact ctcggcatgg acgagctgta caagtaaatc gatagatcct aatcaacctc
     6721 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6781 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6841 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6901 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6961 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7021 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7081 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7141 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7201 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7261 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     7321 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7381 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     7441 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7501 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7561 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7621 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7681 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     7741 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     8161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
     9781 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     9841 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
     9901 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9961 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10021 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    10081 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10141 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10201 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10261 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10321 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    10381 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    10441 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    10501 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10561 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    10621 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    10681 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    10741 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    10801 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10861 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10921 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    10981 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    11041 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    11101 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    11161 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11221 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    11281 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    11341 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    11401 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11461 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    11521 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    11581 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    11641 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    11701 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    11761 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    11821 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11881 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11941 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    12001 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    12061 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    12121 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    12181 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    12241 tcccaacccc gaggggaccc gacaggcccg aaggaataga
//
