LOCUS       sequence-237700-       10492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mECitrine under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP606
FEATURES             Location/Qualifiers
     source          1..10492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1569..1585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1569..1585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1592..1616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3044..3064)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3092..3288)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3294..4109)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3294..4109)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4017..4036
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4119..4141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4130..4146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4155..4160,4161..4176,4177..4182))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      4537..5125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5026..5045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5188..6351
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6374..6394
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7730..7754
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7900..7911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9851..9868
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9852..9868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9893..10492
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 ctttgctcta gaatggtttc taagggagag gagcttttca ctggagtggt cccaatcctc
       61 gtcgagctcg acggagacgt caacggacac aagttctccg tctccggaga gggagaggga
      121 gacgccacct acggaaagct caccctcaag ctcatctgca ccaccggaaa gctcccagtc
      181 ccatggccaa ccctcgtcac caccctcgga tacggactca tgtgcttcgc ccgttaccca
      241 gaccacatga agcaacacga cttcttcaag tccgccatgc cagagggata cgtccaagag
      301 cgtaccatct tcttcaagga cgacggaaac tacaagaccc gtgccgaggt caagttcgag
      361 ggagacaccc tcgtcaaccg tatcgagctc aaggtaagtt taaacatata tatactaact
      421 aaccctgatt atttaaattt tcagggaatc gacttcaagg aggacggaaa catcctcgga
      481 cacaagctcg agtacaacta caactcccac aacgtctaca tcatggccga caagcaaaag
      541 aacggaatca aggtcaactt caagatccgt cacaacatcg aggacggatc cgtccaactc
      601 gccgaccact accaacaaaa caccccaatc ggagacggac cagtcctcct cccagacaac
      661 cactacctct cctaccaatc caagctctcc aaggacccaa acgagaagcg tgaccacatg
      721 gtcctcctcg agttcgtcac cgccgccggc atcacccacg gaatggatga gctctacaag
      781 taaggccgct gtcatcagac atctcgcgcc cgtgcctctg acttctaagt ccaattactc
      841 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
      901 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
      961 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     1021 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     1081 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     1141 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     1201 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     1261 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta
     1321 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     1381 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     1441 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     1501 ttcccaatat accaaacata actgtttgcc cactagtgag tcgtattacg tagcttggcg
     1561 taatcatggt catagctgtt tcctgcccgt gacccagctt tcttgtacaa agtggtaagt
     1621 gcaagtaaga tcagtgtttg tttcgatatc agtctgtttc gtaacggtct tctgtataac
     1681 tacaaaaaag aataaaaaac cgtatcacaa ctgagaaaaa agacaaaact tattgaacag
     1741 tgttcctttc aaaaaattat tttgtaaaaa atcgagtttt tctccaaaat aacgcacgca
     1801 atcaaaaaca agaaaactaa cgtgttctcc tgggcgtgga actccttatc aagattacca
     1861 ttattgtctg tcagaattga tatttcaatt atttcatatc tagacaaacc cgggtgtctt
     1921 gcatttatta gtactgtgag tttacataca aacgaggctt actcagataa catagagtca
     1981 cctctaatta gcaaaaaccc atttctaatt agctatttcg accagttcta aagtcaccgt
     2041 aattagaaat ggtttttgta gcttttctaa ttagaaatga ccccgagata agtgaaatat
     2101 ggcattttct gggttttcta gtaagaaatg atcccgcacg tagtgattcc gcgttacaag
     2161 ccaaattcaa attaaaaaag tatattacac aattcaagtt acattatttt caggaaacaa
     2221 tggcgaagag caaagcagag attcaaaagg aggaaagaga caacagatat gaacgaagac
     2281 ttcttgaaaa gagaaagaga catgaggtga gtaaccatga taataaataa tcaagtaagg
     2341 gctatagatt tataaattct ggagttaaca gtttcgaaaa aaagtagttt tcatatgtgc
     2401 gaaggaatat caaaaatagg ggaatagcct aaacgagtga gaaaattaaa tgtttgttat
     2461 tttttcaaaa atgtctgcag ggccgttatc ctttcaaatg cactctatca aacatagatc
     2521 atatccatca tatcttatcc agaatttata tcatatccaa gattgagagc gtttcaagga
     2581 aaatctcaaa caaccataca attttgtaga tcgacaacaa ggcctccact tcttcatcga
     2641 tggcctctgc agcacctgca ccaccaagtt tgtatttttc atattaatat aaagtgagcc
     2701 acgcatagtc gtttttagga tttttgccag atcgatgtcg cgcaaacaga ctgttttttt
     2761 ttctttgaaa gccgtgcgtg acatgggcca cggattgtaa cgccgcgctg gatggataaa
     2821 aatttattca gttttttagc ctttacaaaa tatggttcga ctactttctt tatttttaca
     2881 tttttatagg tgctcattga gctgagatat tttgggtata atcggaaaat cgctgatttt
     2941 ggtcattttt ctgcactaaa gattacacaa aaagttcagt atttagattc attgttgaaa
     3001 attggggatg atctacagat acatatcgcc gtgttcaaaa acacaacttt attatacata
     3061 gttgataatt cgatggtatc agctcactca aagtatagcg accagcattc acatacgatt
     3121 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
     3181 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
     3241 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattagaaa
     3301 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     3361 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     3421 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     3481 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     3541 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     3601 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     3661 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     3721 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     3781 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     3841 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     3901 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     3961 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     4021 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag
     4081 caagacgttt cccgttgaat atggctcatt ttatgtttcc tgtgtgaaat tgttatccgc
     4141 tcacaattcc acagattata cgagccgatg attaattgtc aacagctctt tatgttcgga
     4201 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     4261 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     4321 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     4381 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     4441 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     4501 aaggagggta ttctgggcct ccatgtcggg atcttcttga gatccttttt ttctgcgcgt
     4561 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4621 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4681 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4741 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4801 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4861 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4921 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4981 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5041 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5101 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5161 cttttgctgg ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa
     5221 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     5281 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     5341 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     5401 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     5461 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     5521 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     5581 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     5641 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     5701 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     5761 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     5821 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     5881 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     5941 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     6001 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     6061 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
     6121 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata
     6181 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     6241 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     6301 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gcgccaggga
     6361 tccgccaagc tatcaacttt gtatagaaaa gttgtgtcgg attatgggct cttcttttat
     6421 cctctacttt ctttctagaa aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa
     6481 ctgcaatggg tttagcttat gtttctggaa caattactgg tggaattgtg attgaaaagt
     6541 aaggttatat ttcatttcaa taaatgttac cacagtataa caaaaacaag ttacattgtt
     6601 cagagtatgt ctgaatgtat tattattatt gtatttgtgc atcagtactg tagttgtact
     6661 gaagaagggt ttaaaataat ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca
     6721 gttaggatgt ttggacatag ttttattaga taatcgtgaa ctaactcact ataaaaaccc
     6781 gctaactcat tgaataagat agttttaaac gttcaaaatt tgaagacaaa attgtatgaa
     6841 taagtttaca gagttggtcg acgcaagctt ctccttctct tcacatttct caataattta
     6901 gctctaattg cattcgtatt cttcgcaaaa atacgtattc tgattgaccc gatgaaatac
     6961 ggatgcctag ttgcattgat catctacggt tatacttatg ggtgaggttt ttcaaatata
     7021 ttttgattta atcgaataat gaaatttcag aactggagtt ggtccaattt cgtggtttat
     7081 atcctctgaa ctggtacctc aaaaacatcg tagtattgct caatctgtgg catattcaat
     7141 gaatactgta atggtggtta tcagtacatt tacagtatta cctctatatt ccgttattgg
     7201 tagttatgct ttccttattc tatattcaat tccttcattt ataagtatgc ttatattatt
     7261 tcgctactta ccggaaacca aaggacgaga gattcatgat attgtgaatg aacttaaaaa
     7321 aaaatgaaaa cctttgaaaa cgttcatacg gccgaaattt tacttgatcg agccaattga
     7381 acttttgtta agacttacag aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc
     7441 gaaaatgaac aggaaaaaga agaaaaatac acccgaagta tcatataaaa attaaaaaga
     7501 ttgtttgacc tggcggaact tttttgcaaa aactctgaat atccatggca catgcttcgt
     7561 gcaaaacagg atccaaatga atatcaacaa gagattcttg aagacgacga gccacttgct
     7621 cagagcattg cttatctcga atgagaccct tgttaaaatc atttttcaaa cattatatga
     7681 cattgtcgaa atgtcctcct gattccatga tggtagcaaa ctcacttcgt caagtttgta
     7741 caaaaaagca ggcttgagcc aatttatcca agtccttgta aaaagtattc gaaaattgtt
     7801 aacggataaa atgtttatta taatatcaaa aacaatttgt cagttgacca ctttttgata
     7861 tatttgacag aaaccgggat gaattggctc aaagttaggg cgcccttcta ttacagggtt
     7921 tctgataaca aaccggttat taactcccaa caagggatga tttcaattca tcatgctcaa
     7981 attgacccaa attaagttac atgacaaatt catcgccctt ttcaactctt cttggctcat
     8041 catctgttat tctgttccta ttctgtctgc accctatacc cctttgcata ctctctcgtc
     8101 attcctttct tggatagtgt cttcttctcc ccagctctgc tacttctatg acttgcgccc
     8161 gctgcttttc cgctcgttcc tctcctctct gacgtcttct tctgcttctt ttctccttcc
     8221 tttttccctt ctcaatcttc ctctttttcc catttctgtc aatcattcga agaagaagaa
     8281 gaagaagacc ctcattcatt tcattttttt ttctgtcggt gtgtgctgct cggttaagag
     8341 tgagctctct attccacgtc ttcttctttt tcttcttcga ttcgaatcaa tcactccacc
     8401 aaaacgcatt cgtttttggg attcaccccg cggttcgcaa taggtttctt tttcaaatta
     8461 ttagcgttat aaatagaaaa atggtggagt ttcaataaaa atgataattt acaaagtgta
     8521 ttttgattac atgtactcaa aaaggttgaa atttttcata ccagttttcc ggaaatccat
     8581 ctgatatcat tatcgtattt ttctttttaa aaatgttttc aaaaaaaaac aaaatatagc
     8641 tggttatttg gcaccctcta attaccattt tcctgtcaca ccacactctt ttccttcttc
     8701 cacttctttc cgttttcagc cgcttccaac caaaccgata tgaaagccga gcaacaacga
     8761 tcgattccac ccggctcggc gaccttcccg tcgcaggtga gactcagaaa actagagaaa
     8821 ccgctcaact aactcttgat atccgatttc attcttttct cttttctttt tgttgaactt
     8881 ttccatattt ccagatgcca cggccaccac caagcaccga acaaggaatc acaacggaat
     8941 cggagcttgc gaagaaagct caaatcactc cgaacgacgt tttagcactt ccgggaatca
     9001 ctcaaggtat cttgctccct ttggatttca gtttaaaaca taaatttagg attcttatgc
     9061 tccccatctg cgaacatcta taacatcgag ttcaccaagt tccaaatccg tgatctggac
     9121 actgagcaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg
     9181 ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt gaaactcaaa
     9241 acggtcggag caactgtgga gttcaaagta ggagacatcc caatccatca tttccgaatg
     9301 atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga atttggattc
     9361 tgtattccga actcacgaaa caactgtgaa catatctatg agttccctca actctctcaa
     9421 caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc tttttcagtg
     9481 gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt cgtcgataac
     9541 aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatatt taatacaaaa
     9601 atgttctgga taattattct gtcgaataga aaaaaaactc caaatgtgat taaattccaa
     9661 taattcctgt ctagtttgtt ccttcccctt ccccttctca tgttcaatgc attcctaagc
     9721 ttttcagttc cccccttgtt tctatatttt ttcgctgtcc tgtcacactc gctaaaaaca
     9781 ctaatcacac ggaaatctgt tttcaataaa aactccaact ttaactcatt ttcaatttca
     9841 actgcacggg tgtaaaacga cggccagtcc gtaatacgac tcacttaagg ccgcaccttt
     9901 ggtcttttat tgtcaacttc cattggttct tccattgttt ctgttaaatt aatgaatttt
     9961 tcataaaata aagacattat acaatataaa aatgaagaat ttattgaaaa taaactgcca
    10021 gagagaaaaa gtatgcaaca ctcccgccga gagtgtttga aatggtgtac ggtacatttt
    10081 cgtgctagga gttagatgtg caggcagcaa cgagaggggg agagattttt ttgggccttg
    10141 tgaaattaac gtgagttttc tggtcatctg actaatcatg ttggtttttt gttggtttat
    10201 tttgttttta tctttgtttt tatccagatt aggaaattta aattttatga atttataatg
    10261 aggtcaaaca ttcagtccca gcgtttttcc tgttctcact gtttagtcga atttttattt
    10321 taggctttca acaaatgttc taactgtctt atttgtgacc tcacttttta tattttttta
    10381 atttttaaaa atattagaag tttctaggat aattttttcg acttttattc tctctaccgt
    10441 ccgcactctt cttactttta aattaaattg tttttttttc agttgggaaa ca
//
