LOCUS       sequence-237702-       11719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E545K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E545K
FEATURES             Location/Qualifiers
     source          1..11719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    128..245
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        293..1471
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          524..1462
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1419..1439
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1491..1515
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4726..4750)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4803..4844
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4865..5432
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4882..5434
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5026..5043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5253..5272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5446..5448,5449..5451,5452..6165)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5446..5448,5449..5451,5452..6162)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5491..5512)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5752..5771)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6099..6120
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6179..6767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6232..6252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6650..6661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6842..7075
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7194..7212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7280..7384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7385..7453,7454..8245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7603..7622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8416..9004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8905..8924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9158..9175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9415..9436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9451..9456,9457..9474,9475..9481)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9489..9505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9494..9516
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9513..9529
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9513..9529
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9579..9598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9790..10423
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10470..10595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11092..11325
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11510..11554
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
       61 tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca
      121 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      181 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      241 attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt gaggctccgg
      301 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      361 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      421 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      481 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      541 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      601 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      661 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      721 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      781 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
      841 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
      901 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
      961 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1021 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1081 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1141 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1201 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1261 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1321 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1381 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1441 aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca acaagtttgt
     1501 acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg ggcatccact
     1561 tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata gtgactttag
     1621 aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa gaagcaagaa
     1681 aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta agtgttactc
     1741 aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac cttcggcttt
     1801 ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag atcctcaatc
     1861 gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt aaagatccag
     1921 aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg gatcttaggg
     1981 acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta gaatcttcac
     2041 cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata gtggtgatct
     2101 gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc aaccatgact
     2161 gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt atgttgctat
     2221 cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt ttaaaagtgt
     2281 gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag tatataagaa
     2341 gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa agcctttatt
     2401 ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt tccacagcta
     2461 caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat agtgcactca
     2521 gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt gataagatct
     2581 atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg aacactcaaa
     2641 gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata tacattcctg
     2701 atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc cgaaagggtg
     2761 ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat tacacagaca
     2821 ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctcatgga ttagaagatt
     2881 tgctgaaccc tattggtgtt actggatcaa atccaaataa agaaactcca tgcttagagt
     2941 tggagtttga ctggttcagc agtgtggtaa agttcccaga tatgtcagtg attgaagagc
     3001 atgccaattg gtctgtatcc cgagaagcag gatttagcta ttcccacgca ggactgagta
     3061 acagactagc tagagacaat gaattaaggg aaaatgacaa agaacagctc aaagcaattt
     3121 ctacacgaga tcctctctct gaaatcacta agcaggagaa agattttcta tggagtcaca
     3181 gacactattg tgtaactatc cccgaaattc tacccaaatt gcttctgtct gttaaatgga
     3241 attctagaga tgaagtagcc cagatgtatt gcttggtaaa agattggcct ccaatcaaac
     3301 ctgaacaggc tatggaactt ctggactgta attacccaga tcctatggtt cgaggttttg
     3361 ctgttcggtg cttggaaaaa tatttaacag atgacaaact ttctcagtat ttaattcagc
     3421 tagtacaggt cctaaaatat gaacaatatt tggataactt gcttgtgaga tttttactga
     3481 agaaagcatt gactaatcaa aggattgggc actttttctt ttggcattta aaatctgaga
     3541 tgcacaataa aacagttagc cagaggtttg gcctgctttt ggagtcctat tgtcgtgcat
     3601 gtgggatgta tttgaagcac ctgaataggc aagtcgaggc aatggaaaag ctcattaact
     3661 taactgacat tctcaaacag gagaagaagg atgaaacaca aaaggtacag atgaagtttt
     3721 tagttgagca aatgaggcga ccagatttca tggatgctct acagggcttt ctgtctcctc
     3781 taaaccctgc tcatcaacta ggaaacctca ggcttgaaga gtgtcgaatt atgtcctctg
     3841 caaaaaggcc actgtggttg aattgggaga acccagacat catgtcagag ttactgtttc
     3901 agaacaatga gatcatcttt aaaaatgggg atgatttacg gcaagatatg ctaacacttc
     3961 aaattattcg tattatggaa aatatctggc aaaatcaagg tcttgatctt cgaatgttac
     4021 cttatggttg tctgtcaatc ggtgactgtg tgggacttat tgaggtggtg cgaaattctc
     4081 acactattat gcaaattcag tgcaaaggcg gcttgaaagg tgcactgcag ttcaacagcc
     4141 acacactaca tcagtggctc aaagacaaga acaaaggaga aatatatgat gcagccattg
     4201 acctgtttac acgttcatgt gctggatact gtgtagctac cttcattttg ggaattggag
     4261 atcgtcacaa tagtaacatc atggtgaaag acgatggaca actgtttcat atagattttg
     4321 gacacttttt ggatcacaag aagaaaaaat ttggttataa acgagaacgt gtgccatttg
     4381 ttttgacaca ggatttctta atagtgatta gtaaaggagc ccaagaatgc acaaagacaa
     4441 gagaatttga gaggtttcag gagatgtgtt acaaggctta tctagctatt cgacagcatg
     4501 ccaatctctt cataaatctt ttctcaatga tgcttggctc tggaatgcca gaactacaat
     4561 cttttgatga cattgcatac attcgaaaga ccctagcctt agataaaact gagcaagagg
     4621 ctttggagta tttcatgaaa caaatgaatg atgcacatca tggtggctgg acaacaaaaa
     4681 tggattggat cttccacaca attaaacagc atgcattgaa ctagaaccca gctttcttgt
     4741 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     4801 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     4861 gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4921 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4981 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     5041 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     5101 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5161 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5221 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5281 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5341 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5401 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt gagcaagggc
     5461 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5521 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5581 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5641 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5701 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5761 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5821 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5881 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5941 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     6001 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     6061 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     6121 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga tagatcctaa
     6181 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6241 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6301 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6361 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6421 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6481 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6541 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6601 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6661 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6721 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     6781 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6841 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6901 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6961 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7021 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7081 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7141 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     7201 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7381 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7441 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7501 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7561 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7621 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7681 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7741 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7801 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7861 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7921 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7981 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8041 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8101 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8161 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8221 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8281 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8341 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8401 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8461 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8521 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8581 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8641 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8701 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8761 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8821 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8881 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8941 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9001 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9061 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9121 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9181 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9241 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9301 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9361 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9421 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9481 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9601 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9661 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9721 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9781 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     9841 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     9901 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     9961 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    10021 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    10081 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    10141 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    10201 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    10261 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10321 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10381 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10441 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    10501 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    10561 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    10621 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    10681 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    10741 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    10801 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    10861 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    10921 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10981 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11041 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11101 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11161 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11221 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11281 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11341 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11401 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11461 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11521 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11581 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11641 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11701 gacccacctc ccaaccccg
//
