LOCUS       sequence-237705-        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid with Constitutively expressed mCherry and 
            CggR-regulated YFP (eCitrine).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Monteiro F, Hubmann G, Takhaveev V, Vedelaar SR, Norder J, Hekelaar 
            J, Saldida J, Litsios A, Wijma HJ, Schmidt A, Heinemann M
  TITLE     Measuring glycolytic flux in single yeast cells with an orthogonal 
            synthetic biosensor.
  JOURNAL   Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071.
  PUBMED    31885198
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2019-12"; volume: "15(12):e9071. doi"; pages: " 
            10.15252/msb.20199071"
COMMENT     Sequence Label: pCggRO-reporter
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2828..2861
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            2916..4272
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2916..3259
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3260..4069
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3327..3346)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      4075..4272
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    4335..4368
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4690..5406
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5409..5425)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5410..5426)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5444..5462)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(5676..5841)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5762..5786
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5864..6574)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6142..6161)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6411..6429
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(6587..6985)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6677..6688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7017..7050)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7086..7105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7087..7105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7115..7132)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7115..7131)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7276..7731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7363..7382)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7573..7594
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7862..8665)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8666..8886)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8912..8931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9031..9053
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctgcag ctgaagcttc gtacgctgca ggtcgacaac
     2821 ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag agatctgttt
     2881 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
     2941 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     3001 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     3061 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     3121 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     3181 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     3241 catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa
     3301 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3361 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3421 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3481 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3541 actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc
     3601 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3661 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3721 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3781 acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca
     3841 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3901 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3961 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     4021 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagtactgac
     4081 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     4141 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     4201 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     4261 gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa aacgagctct
     4321 cgagaaccct taatataact tcgtataatg tatgctatac gaagttatta gggacatcgt
     4381 aacaacacca aaccaaaact tggcgacggg accaggactg tcttaccggg acgtttactg
     4441 tcgctcctgt tgagtgaatc atatatatag aaagcgggtt tttttaaatt cctaacggga
     4501 ctttttttgt cagtccggga ctttaattgt ccgaaaaaat aaaaaagtga tagggtatat
     4561 atttttatat aaacgaggtt aagtgatcgc gggactttcc ctgtcatcgc gggactaccc
     4621 ttgtcatttg agattaaaaa ctatacttct atagacacac aaacacaaat acacacacta
     4681 aattaataaa tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa
     4741 ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct
     4801 acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg
     4861 ccaaccttag tcactacttt aggttatggt ttgatgtgtt ttgctagata cccagatcat
     4921 atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact
     4981 atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat
     5041 accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta
     5101 ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc tgacaaacaa
     5161 aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa
     5221 ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac
     5281 aaccattact tatcctatca atctgcctta tccaaagatc caaacgaaaa gagagaccac
     5341 atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac
     5401 aaataaatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc ttacattcac
     5461 gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     5521 tccctattta ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat
     5581 ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct
     5641 tgagaaggtt ttgggacgct cgggtaccag cgtacgagcg acctcatgct atacctgaga
     5701 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta
     5761 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat
     5821 cataaatcat aagaaattcg cttatttaga agtgtcaaca acgctacttg tacagctcgt
     5881 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
     5941 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
     6001 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
     6061 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
     6121 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
     6181 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
     6241 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     6301 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
     6361 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
     6421 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     6481 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
     6541 tggccatgtt atcctcctcg cccttgctca ccatttttct agaaaactta gattagattg
     6601 ctatgctttc tttctaatga gcaagaggta aaaaaagttg tagtagagca agaaaagtgg
     6661 gactgaaact tgggaaattg aaggccgttt attaacttaa atatcgatgg gaggtcatcg
     6721 aaagagaaaa aaatcaaaaa aagaattttc aagaaaaaga aacgtgataa aaatttttat
     6781 tgcctttttc gacgaagaaa aagaaacgag gcggtctctt ttttcttttc caaaccttta
     6841 gtacgggtaa ttaacgacac cctagaggaa gaaagagggg aaatttagta tgctgtgctt
     6901 gggtgttttg aagtggtacg gcgatgcgcg gagtccgaga agatctggaa gagtaaaaga
     6961 ggagtagaaa cgtttcgaag ctatgcatag gccactagtg gatctgatat cacctaataa
     7021 cttcgtatag catacattat acgaagttat attaagggtt ctcgagagct cgggtaccca
     7081 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7141 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7201 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7261 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7321 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7381 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7441 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7501 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7561 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7621 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7681 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg
     7741 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat aactgatata
     7801 attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt
     7861 tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt
     7921 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt
     7981 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct
     8041 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac
     8101 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa
     8161 cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca
     8221 tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa
     8281 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata
     8341 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga
     8401 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg
     8461 aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt
     8521 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt
     8581 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac
     8641 gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt
     8701 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat
     8761 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat
     8821 caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga
     8881 attgaaaagc tgtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8941 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9001 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9061 cgtcatcacc gaaacgcgcg a
//
