LOCUS       sequence-237706-       10573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mScarlet under EFT3 Promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP602
FEATURES             Location/Qualifiers
     source          1..10573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..600
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     primer_bind     complement(2250..2266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2250..2266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2273..2297)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3725..3745)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(3773..3969)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3975..4790)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3975..4790)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4698..4717
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(4800..4822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4811..4827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4836..4841,4842..4857,4858..4863))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      5218..5806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5707..5726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5869..7032
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    7055..7075
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8411..8435
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8581..8592)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10532..10549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10533..10549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
       61 tgaatttttc ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata
      121 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
      181 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
      241 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
      301 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
      361 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
      421 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
      481 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
      541 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
      601 ctttgctcta gaatggtctc caagggagag gccgtcatca aggagttcat gcgtttcaag
      661 gtccacatgg agggatccat gaacggacac gagttcgaga tcgagggaga gggagaggga
      721 cgtccatacg agggaaccca aaccgccaag ctcaaggtca ccaaggtaag tttaaacata
      781 tatatactaa ctaaccctga ttatttaaat tttcagggag gaccactccc attctcctgg
      841 gacatcctct ccccacaatt catgtacgga tcccgtgcct tcatcaagca cccagccgac
      901 atcccagact actacaagca atccttccca gagggattca agtgggagcg tgtcatgaac
      961 ttcgaggacg gaggagccgt caccgtcacc caagacacct ccctcgagga cggaaccctc
     1021 atctacaagg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
     1081 gtcaagctcc gtggaaccaa cttcccacca gacggaccag tcatgcaaaa gaagaccatg
     1141 ggatgggagg cctccaccga gcgtctctac ccagaggacg gagtcctcaa gggagacatc
     1201 aagatggccc tccgtctcaa ggacggagga cgttacctcg ccgacttcaa ggtaagttta
     1261 aacatgattt tactaactaa ctaatctgat ttaaattttc agaccaccta caaggccaag
     1321 aagccagtcc aaatgccagg agcctacaac gtcgaccgta agctcgacat cacctcccac
     1381 aacgaggact acaccgtcgt cgagcaatac gagcgttccg agggacgtca ctccaccgga
     1441 ggaatggacg agctctacaa gtaaggccgc tgtcatcaga catctcgcgc ccgtgcctct
     1501 gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
     1561 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
     1621 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     1681 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
     1741 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
     1801 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
     1861 tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt
     1921 tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt
     1981 tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta
     2041 aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg
     2101 ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt
     2161 tgccttaaat gacagaatac attcccaata taccaaacat aactgtttgc ccactagtga
     2221 gtcgtattac gtagcttggc gtaatcatgg tcatagctgt ttcctgcccg tgacccagct
     2281 ttcttgtaca aagtggtaag tgcaagtaag atcagtgttt gtttcgatat cagtctgttt
     2341 cgtaacggtc ttctgtataa ctacaaaaaa gaataaaaaa ccgtatcaca actgagaaaa
     2401 aagacaaaac ttattgaaca gtgttccttt caaaaaatta ttttgtaaaa aatcgagttt
     2461 ttctccaaaa taacgcacgc aatcaaaaac aagaaaacta acgtgttctc ctgggcgtgg
     2521 aactccttat caagattacc attattgtct gtcagaattg atatttcaat tatttcatat
     2581 ctagacaaac ccgggtgtct tgcatttatt agtactgtga gtttacatac aaacgaggct
     2641 tactcagata acatagagtc acctctaatt agcaaaaacc catttctaat tagctatttc
     2701 gaccagttct aaagtcaccg taattagaaa tggtttttgt agcttttcta attagaaatg
     2761 accccgagat aagtgaaata tggcattttc tgggttttct agtaagaaat gatcccgcac
     2821 gtagtgattc cgcgttacaa gccaaattca aattaaaaaa gtatattaca caattcaagt
     2881 tacattattt tcaggaaaca atggcgaaga gcaaagcaga gattcaaaag gaggaaagag
     2941 acaacagata tgaacgaaga cttcttgaaa agagaaagag acatgaggtg agtaaccatg
     3001 ataataaata atcaagtaag ggctatagat ttataaattc tggagttaac agtttcgaaa
     3061 aaaagtagtt ttcatatgtg cgaaggaata tcaaaaatag gggaatagcc taaacgagtg
     3121 agaaaattaa atgtttgtta ttttttcaaa aatgtctgca gggccgttat cctttcaaat
     3181 gcactctatc aaacatagat catatccatc atatcttatc cagaatttat atcatatcca
     3241 agattgagag cgtttcaagg aaaatctcaa acaaccatac aattttgtag atcgacaaca
     3301 aggcctccac ttcttcatcg atggcctctg cagcacctgc accaccaagt ttgtattttt
     3361 catattaata taaagtgagc cacgcatagt cgtttttagg atttttgcca gatcgatgtc
     3421 gcgcaaacag actgtttttt tttctttgaa agccgtgcgt gacatgggcc acggattgta
     3481 acgccgcgct ggatggataa aaatttattc agttttttag cctttacaaa atatggttcg
     3541 actactttct ttatttttac atttttatag gtgctcattg agctgagata ttttgggtat
     3601 aatcggaaaa tcgctgattt tggtcatttt tctgcactaa agattacaca aaaagttcag
     3661 tatttagatt cattgttgaa aattggggat gatctacaga tacatatcgc cgtgttcaaa
     3721 aacacaactt tattatacat agttgataat tcgatggtat cagctcactc aaagtatagc
     3781 gaccagcatt cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc
     3841 tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag
     3901 aacaactaca atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt
     3961 attgtcagta ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     4021 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     4081 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     4141 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     4201 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     4261 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     4321 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     4381 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     4441 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     4501 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     4561 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     4621 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     4681 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     4741 gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat tttatgtttc
     4801 ctgtgtgaaa ttgttatccg ctcacaattc cacagattat acgagccgat gattaattgt
     4861 caacagctct ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg
     4921 aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg
     4981 cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtctg
     5041 cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa
     5101 tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga
     5161 gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcgg gatcttcttg
     5221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5341 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5821 ggccttttta cggttcctgg ccttttgctg gccgaaaagt gccacctgaa cgaagcatct
     5881 gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     5941 ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga
     6001 atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     6061 agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac
     6121 aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta
     6181 acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat
     6241 aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct
     6301 cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg
     6361 gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca
     6421 tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac
     6481 ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt
     6541 tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag
     6601 agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg
     6661 atgggtaggt tatataggga tatagcacag agatatatag caaagagata cttttgagca
     6721 atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt
     6781 ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct
     6841 atactttcta gctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
     6901 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
     6961 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
     7021 tgcttaaatg cgcgccaggg atccgccaag ctatcaactt tgtatagaaa agttgtgtcg
     7081 gattatgggc tcttctttta tcctctactt tctttctaga aaatgcaaat gttgagtcag
     7141 aaattgctga atggagtaca actgcaatgg gtttagctta tgtttctgga acaattactg
     7201 gtggaattgt gattgaaaag taaggttata tttcatttca ataaatgtta ccacagtata
     7261 acaaaaacaa gttacattgt tcagagtatg tctgaatgta ttattattat tgtatttgtg
     7321 catcagtact gtagttgtac tgaagaaggg tttaaaataa tctcaacgtt tttaagacga
     7381 gttagtgaga atacggtcgc agttaggatg tttggacata gttttattag ataatcgtga
     7441 actaactcac tataaaaacc cgctaactca ttgaataaga tagttttaaa cgttcaaaat
     7501 ttgaagacaa aattgtatga ataagtttac agagttggtc gacgcaagct tctccttctc
     7561 ttcacatttc tcaataattt agctctaatt gcattcgtat tcttcgcaaa aatacgtatt
     7621 ctgattgacc cgatgaaata cggatgccta gttgcattga tcatctacgg ttatacttat
     7681 gggtgaggtt tttcaaatat attttgattt aatcgaataa tgaaatttca gaactggagt
     7741 tggtccaatt tcgtggttta tatcctctga actggtacct caaaaacatc gtagtattgc
     7801 tcaatctgtg gcatattcaa tgaatactgt aatggtggtt atcagtacat ttacagtatt
     7861 acctctatat tccgttattg gtagttatgc tttccttatt ctatattcaa ttccttcatt
     7921 tataagtatg cttatattat ttcgctactt accggaaacc aaaggacgag agattcatga
     7981 tattgtgaat gaacttaaaa aaaaatgaaa acctttgaaa acgttcatac ggccgaaatt
     8041 ttacttgatc gagccaattg aacttttgtt aagacttaca gaaaagtgag agattttgtt
     8101 acaaaaaagt gagaaaaaag cgaaaatgaa caggaaaaag aagaaaaata cacccgaagt
     8161 atcatataaa aattaaaaag attgtttgac ctggcggaac ttttttgcaa aaactctgaa
     8221 tatccatggc acatgcttcg tgcaaaacag gatccaaatg aatatcaaca agagattctt
     8281 gaagacgacg agccacttgc tcagagcatt gcttatctcg aatgagaccc ttgttaaaat
     8341 catttttcaa acattatatg acattgtcga aatgtcctcc tgattccatg atggtagcaa
     8401 actcacttcg tcaagtttgt acaaaaaagc aggcttgagc caatttatcc aagtccttgt
     8461 aaaaagtatt cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg
     8521 tcagttgacc actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg
     8581 gcgcccttct attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg
     8641 atttcaattc atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct
     8701 tttcaactct tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac
     8761 ccctttgcat actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg
     8821 ctacttctat gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc
     8881 ttctgcttct tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt
     8941 caatcattcg aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg
     9001 tgtgtgctgc tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg
     9061 attcgaatca atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca
     9121 ataggtttct ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa
     9181 aatgataatt tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat
     9241 accagttttc cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt
     9301 caaaaaaaaa caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac
     9361 accacactct tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat
     9421 atgaaagccg agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg
     9481 agactcagaa aactagagaa accgctcaac taactcttga tatccgattt cattcttttc
     9541 tcttttcttt ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg
     9601 aacaaggaat cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg
     9661 ttttagcact tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac
     9721 ataaatttag gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag
     9781 ttccaaatcc gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac
     9841 gatcaggaga atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct
     9901 ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg agttcaaagt aggagacatc
     9961 ccaatccatc atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt
    10021 tttgactttg aatttggatt ctgtattccg aactcacgaa acaactgtga acatatctat
    10081 gagttccctc aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa
    10141 agactaatct ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac
    10201 agcttctatt tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat
    10261 gcataaatat ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact
    10321 ccaaatgtga ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc
    10381 atgttcaatg cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc
    10441 ctgtcacact cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac
    10501 tttaactcat tttcaatttc aactgcacgg gtgtaaaacg acggccagtc cgtaatacga
    10561 ctcacttaag gcc
//
