LOCUS       sequence-237709-       11833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PDGFRB T369M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PDGFRB-T369M
FEATURES             Location/Qualifiers
     source          1..11833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    144..261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        309..1487
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          540..1478
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1435..1455
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1507..1531
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3070..3216
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     protein_bind    complement(4856..4880)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4933..4974
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4995..5562
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5012..5564
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5156..5173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5383..5402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5576..5578,5579..5581,5582..6295)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5576..5578,5579..5581,5582..6292)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5621..5642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5882..5901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6229..6250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6309..6897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6362..6382)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6780..6791)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6972..7205
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7324..7342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7410..7514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7515..7583,7584..8375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7733..7752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8546..9134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9035..9054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9288..9305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9545..9566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9581..9586,9587..9604,9605..9611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9619..9635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9624..9646
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9643..9659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9643..9659
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9709..9728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9920..10553
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10600..10725
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11222..11455
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11640..11684
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
       61 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca
      121 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      181 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      241 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact
      301 agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      361 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      421 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      481 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      541 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      601 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      661 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      721 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      781 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      841 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      901 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      961 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     1021 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     1081 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     1141 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     1201 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     1261 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1321 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1381 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1441 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc ggccctgcag
     1501 atatcaacaa gtttgtacaa aaaagcaggc accatgcggc ttccgggtgc gatgccagct
     1561 ctggccctca aaggcgagct gctgttgctg tctctcctgt tacttctgga accacagatc
     1621 tctcagggcc tggtcgtcac acccccgggg ccagagcttg tcctcaatgt ctccagcacc
     1681 ttcgttctga cctgctcggg ttcagctccg gtggtgtggg aacggatgtc ccaggagccc
     1741 ccacaggaaa tggccaaggc ccaggatggc accttctcca gcgtgctcac actgaccaac
     1801 ctcactgggc tagacacggg agaatacttt tgcacccaca atgactcccg tggactggag
     1861 accgatgagc ggaaacggct ctacatcttt gtgccagatc ccaccgtggg cttcctccct
     1921 aatgatgccg aggaactatt catctttctc acggaaataa ctgagatcac cattccatgc
     1981 cgagtaacag acccacagct ggtggtgaca ctgcacgaga agaaagggga cgttgcactg
     2041 cctgtcccct atgatcacca acgtggcttt tttggtatct ttgaggacag aagctacatc
     2101 tgcaaaacca ccattgggga cagggaggtg gattctgatg cctactatgt ctacagactc
     2161 caggtgtcat ccatcaacgt ctctgtgaac gcagtgcaga ctgtggtccg ccagggtgag
     2221 aacatcaccc tcatgtgcat tgtgatcggg aatgaggtgg tcaacttcga gtggacatac
     2281 ccccgcaaag aaagtgggcg gctggtggag ccggtgactg acttcctctt ggatatgcct
     2341 taccacatcc gctccatcct gcacatcccc agtgccgagt tagaagactc ggggacctac
     2401 acctgcaatg tgacggagag tgtgaatgac catcaggatg aaaaggccat caacatcacc
     2461 gtggttgaga gcggctacgt gcggctcctg ggagaggtgg gcacactaca atttgctgag
     2521 ctgcatcgga gccggacact gcaggtagtg ttcgaggcct acccaccgcc cactgtcctg
     2581 tggttcaaag acaaccgcac cctgggcgac tccagcgctg gcgaaatcgc cctgtccatg
     2641 cgcaacgtgt cggagacccg gtatgtgtca gagctgacac tggttcgcgt gaaggtggca
     2701 gaggctggcc actacaccat gcgggccttc catgaggatg ctgaggtcca gctctccttc
     2761 cagctacaga tcaatgtccc tgtccgagtg ctggagctaa gtgagagcca ccctgacagt
     2821 ggggaacaga cagtccgctg tcgtggccgg ggcatgcccc agccgaacat catctggtct
     2881 gcctgcagag acctcaaaag gtgtccacgt gagctgccgc ccacgctgct ggggaacagt
     2941 tccgaagagg agagccagct ggagactaac gtgacgtact gggaggagga gcaggagttt
     3001 gaggtggtga gcacactgcg tctgcagcac gtggatcggc cactgtcggt gcgctgcacg
     3061 ctgcgcaacg ctgtgggcca ggacacgcag gaggtcatcg tggtgccaca ctccttgccc
     3121 tttaaggtgg tggtgatctc agccatcctg gccctggtgg tgctcaccat catctccctt
     3181 atcatcctca tcatgctttg gcagaagaag ccacgttacg agatccgatg gaaggtgatt
     3241 gagtctgtga gctctgacgg ccatgagtac atctacgtgg accccatgca gctgccctat
     3301 gactccacgt gggagctgcc gcgggaccag cttgtgctgg gacgcaccct cggctctggg
     3361 gcctttgggc aggtggtgga ggccacggct catggcctga gccattctca ggccacgatg
     3421 aaagtggccg tcaagatgct taaatccaca gcccgcagca gtgagaagca agcccttatg
     3481 tcggagctga agatcatgag tcaccttggg ccccacctga acgtggtcaa cctgttgggg
     3541 gcctgcacca aaggaggacc catctatatc atcactgagt actgccgcta cggagacctg
     3601 gtggactacc tgcaccgcaa caaacacacc ttcctgcagc accactccga caagcgccgc
     3661 ccgcccagcg cggagctcta cagcaatgct ctgcccgttg ggctccccct gcccagccat
     3721 gtgtccttga ccggggagag cgacggtggc tacatggaca tgagcaagga cgagtcggtg
     3781 gactatgtgc ccatgctgga catgaaagga gacgtcaaat atgcagacat cgagtcctcc
     3841 aactacatgg ccccttacga taactacgtt ccctctgccc ctgagaggac ctgccgagca
     3901 actttgatca acgagtctcc agtgctaagc tacatggacc tcgtgggctt cagctaccag
     3961 gtggccaatg gcatggagtt tctggcctcc aagaactgcg tccacagaga cctggcggct
     4021 aggaacgtgc tcatctgtga aggcaagctg gtcaagatct gtgactttgg cctggctcga
     4081 gacatcatgc gggactcgaa ttacatctcc aaaggcagca cctttttgcc tttaaagtgg
     4141 atggctccgg agagcatctt caacagcctc tacaccaccc tgagcgacgt gtggtccttc
     4201 gggatcctgc tctgggagat cttcaccttg ggtggcaccc cttacccaga gctgcccatg
     4261 aacgagcagt tctacaatgc catcaaacgg ggttaccgca tggcccagcc tgcccatgcc
     4321 tccgacgaga tctatgagat catgcagaag tgctgggaag agaagtttga gattcggccc
     4381 cccttctccc agctggtgct gcttctcgag agactgttgg gcgaaggtta caaaaagaag
     4441 taccagcagg tggatgagga gtttctgagg agtgaccacc cagccatcct tcggtcccag
     4501 gcccgcttgc ctgggttcca tggcctccga tctcccctgg acaccagctc cgtcctctat
     4561 actgccgtgc agcccaatga gggtgacaac gactatatca tccccctgcc tgaccccaaa
     4621 cccgaggttg ctgacgaggg cccactggag ggttccccca gcctagccag ctccaccctg
     4681 aatgaagtca acacctcctc aaccatctcc tgtgacagcc ccctggagcc ccaggacgaa
     4741 ccagagccag agccccagct tgagctccag gtggagccgg agccagagct ggaacagttg
     4801 ccggattcgg ggtgccctgc gcctcgggcg gaagcagagg atagcttcct gtagaaccca
     4861 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     4921 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     4981 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5041 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5101 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5161 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5221 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5281 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5341 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5401 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5461 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     5521 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt
     5581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5641 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5821 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6001 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     6061 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     6121 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6181 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6241 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga
     6301 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6361 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6421 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6481 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6541 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6601 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6661 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6721 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6781 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6841 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6901 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6961 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7021 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7081 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7141 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7201 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7261 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     7321 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7381 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7741 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9241 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9301 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9361 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9421 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9481 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9541 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9601 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     9661 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9721 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     9781 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     9841 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     9901 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
     9961 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10021 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10081 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10141 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10201 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10261 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    10321 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    10381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10561 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    10621 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    10681 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    10741 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    10801 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    10861 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    10921 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    10981 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11041 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
    11101 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    11161 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    11221 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    11281 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    11341 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    11401 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    11461 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    11521 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    11581 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    11641 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    11701 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    11761 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    11821 tatcgtttca gac
//
