LOCUS       sequence-237711-       11816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E545Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E545Q
FEATURES             Location/Qualifiers
     source          1..11816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    530..763
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             948..992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1285..1402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1450..2628
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1681..2619
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2576..2596
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2648..2672
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5883..5907)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5915..5933
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5958..5977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5959..5977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5983..6003)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6057..6098
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6119..6686
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6136..6688
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6280..6297)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6507..6526
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6700..6702,6703..6705,6706..7419)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6700..6702,6703..6705,6706..7416)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6745..6766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7006..7025)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7353..7374
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7433..8021
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7486..7506)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7904..7915)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8096..8329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8448..8466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8534..8638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8639..8707,8708..9499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8857..8876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9670..10258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10159..10178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10412..10429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10669..10690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10705..10710,10711..10728,10729..10735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10743..10759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10748..10770
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10767..10783
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10767..10783
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10833..10852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11044..11677
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(11724..11816,1..33)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
       61 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      121 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      181 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
      241 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
      301 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
      361 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      421 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      481 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      541 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
      601 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
      661 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
      721 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
      781 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
      841 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
      901 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
      961 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1021 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1081 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1141 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1201 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc
     1261 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1321 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1381 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac
     1441 tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     1501 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     1561 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     1621 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     1681 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     1741 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     1801 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     1861 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     1921 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     1981 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     2041 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     2101 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     2161 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     2221 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     2281 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     2341 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     2401 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     2461 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     2521 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     2581 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccctgca
     2641 gatatcaaca agtttgtaca aaaaagcagg caccatgcct ccaagaccat catcaggtga
     2701 actgtggggc atccacttga tgcccccaag aatcctagta gaatgtttac taccaaatgg
     2761 aatgatagtg actttagaat gcctccgtga ggctacatta ataaccataa agcatgaact
     2821 atttaaagaa gcaagaaaat accccctcca tcaacttctt caagatgaat cttcttacat
     2881 tttcgtaagt gttactcaag aagcagaaag ggaagaattt tttgatgaaa caagacgact
     2941 ttgtgacctt cggctttttc aacccttttt aaaagtaatt gaaccagtag gcaaccgtga
     3001 agaaaagatc ctcaatcgag aaattggttt tgctatcggc atgccagtgt gtgaatttga
     3061 tatggttaaa gatccagaag tacaggactt ccgaagaaat attctgaacg tttgtaaaga
     3121 agctgtggat cttagggacc tcaattcacc tcatagtaga gcaatgtatg tctatcctcc
     3181 aaatgtagaa tcttcaccag aattgccaaa gcacatatat aataaattag ataaagggca
     3241 aataatagtg gtgatctggg taatagtttc tccaaataat gacaagcaga agtatactct
     3301 gaaaatcaac catgactgtg taccagaaca agtaattgct gaagcaatca ggaaaaaaac
     3361 tcgaagtatg ttgctatcct ctgaacaact aaaactctgt gttttagaat atcagggcaa
     3421 gtatatttta aaagtgtgtg gatgtgatga atacttccta gaaaaatatc ctctgagtca
     3481 gtataagtat ataagaagct gtataatgct tgggaggatg cccaatttga tgttgatggc
     3541 taaagaaagc ctttattctc aactgccaat ggactgtttt acaatgccat cttattccag
     3601 acgcatttcc acagctacac catatatgaa tggagaaaca tctacaaaat ccctttgggt
     3661 tataaatagt gcactcagaa taaaaattct ttgtgcaacc tacgtgaatg taaatattcg
     3721 agacattgat aagatctatg ttcgaacagg tatctaccat ggaggagaac ccttatgtga
     3781 caatgtgaac actcaaagag taccttgttc caatcccagg tggaatgaat ggctgaatta
     3841 tgatatatac attcctgatc ttcctcgtgc tgctcgactt tgcctttcca tttgctctgt
     3901 taaaggccga aagggtgcta aagaggaaca ctgtccattg gcatggggaa atataaactt
     3961 gtttgattac acagacactc tagtatctgg aaaaatggct ttgaatcttt ggccagtacc
     4021 tcatggatta gaagatttgc tgaaccctat tggtgttact ggatcaaatc caaataaaga
     4081 aactccatgc ttagagttgg agtttgactg gttcagcagt gtggtaaagt tcccagatat
     4141 gtcagtgatt gaagagcatg ccaattggtc tgtatcccga gaagcaggat ttagctattc
     4201 ccacgcagga ctgagtaaca gactagctag agacaatgaa ttaagggaaa atgacaaaga
     4261 acagctcaaa gcaatttcta cacgagatcc tctctctgaa atcactcagc aggagaaaga
     4321 ttttctatgg agtcacagac actattgtgt aactatcccc gaaattctac ccaaattgct
     4381 tctgtctgtt aaatggaatt ctagagatga agtagcccag atgtattgct tggtaaaaga
     4441 ttggcctcca atcaaacctg aacaggctat ggaacttctg gactgtaatt acccagatcc
     4501 tatggttcga ggttttgctg ttcggtgctt ggaaaaatat ttaacagatg acaaactttc
     4561 tcagtattta attcagctag tacaggtcct aaaatatgaa caatatttgg ataacttgct
     4621 tgtgagattt ttactgaaga aagcattgac taatcaaagg attgggcact ttttcttttg
     4681 gcatttaaaa tctgagatgc acaataaaac agttagccag aggtttggcc tgcttttgga
     4741 gtcctattgt cgtgcatgtg ggatgtattt gaagcacctg aataggcaag tcgaggcaat
     4801 ggaaaagctc attaacttaa ctgacattct caaacaggag aagaaggatg aaacacaaaa
     4861 ggtacagatg aagtttttag ttgagcaaat gaggcgacca gatttcatgg atgctctaca
     4921 gggctttctg tctcctctaa accctgctca tcaactagga aacctcaggc ttgaagagtg
     4981 tcgaattatg tcctctgcaa aaaggccact gtggttgaat tgggagaacc cagacatcat
     5041 gtcagagtta ctgtttcaga acaatgagat catctttaaa aatggggatg atttacggca
     5101 agatatgcta acacttcaaa ttattcgtat tatggaaaat atctggcaaa atcaaggtct
     5161 tgatcttcga atgttacctt atggttgtct gtcaatcggt gactgtgtgg gacttattga
     5221 ggtggtgcga aattctcaca ctattatgca aattcagtgc aaaggcggct tgaaaggtgc
     5281 actgcagttc aacagccaca cactacatca gtggctcaaa gacaagaaca aaggagaaat
     5341 atatgatgca gccattgacc tgtttacacg ttcatgtgct ggatactgtg tagctacctt
     5401 cattttggga attggagatc gtcacaatag taacatcatg gtgaaagacg atggacaact
     5461 gtttcatata gattttggac actttttgga tcacaagaag aaaaaatttg gttataaacg
     5521 agaacgtgtg ccatttgttt tgacacagga tttcttaata gtgattagta aaggagccca
     5581 agaatgcaca aagacaagag aatttgagag gtttcaggag atgtgttaca aggcttatct
     5641 agctattcga cagcatgcca atctcttcat aaatcttttc tcaatgatgc ttggctctgg
     5701 aatgccagaa ctacaatctt ttgatgacat tgcatacatt cgaaagaccc tagccttaga
     5761 taaaactgag caagaggctt tggagtattt catgaaacaa atgaatgatg cacatcatgg
     5821 tggctggaca acaaaaatgg attggatctt ccacacaatt aaacagcatg cattgaacta
     5881 gaacccagct ttcttgtaca aagtggtgat atccaattaa ccctcactaa aggaatagat
     5941 aagattgatt gtgtttgccc tatagtgagt cgtattacca cccaactttt ctatacaaag
     6001 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     6061 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc
     6121 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6181 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6241 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6301 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6361 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6421 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6481 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6541 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     6601 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6661 ttttcctttg aaaaacacga tgataatatg gccacacata tggtgagcaa gggcgaggag
     6721 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     6781 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     6841 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     6901 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     6961 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7021 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7081 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7141 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     7201 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     7261 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     7321 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     7381 gccgggatca ctctcggcat ggacgagctg tacaagtaaa tcgatagatc ctaatcaacc
     7441 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7501 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7561 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7621 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7681 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7741 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7801 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7861 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7921 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7981 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     8041 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8101 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     8161 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8221 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8281 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8341 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8401 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     8461 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8881 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10321 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10381 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10441 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
    10501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    10561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
    10621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10741 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
    10801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10861 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10921 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    10981 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11041 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
    11101 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
    11161 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
    11221 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
    11281 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
    11341 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
    11401 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
    11461 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
    11521 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    11581 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11641 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    11701 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    11761 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcgga
//
