LOCUS       sequence-237717-       11816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA P539R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-P539R
FEATURES             Location/Qualifiers
     source          1..11816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    133..250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        298..1476
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          529..1467
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1424..1444
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1496..1520
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4731..4755)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4763..4781
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4806..4825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4807..4825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4831..4851)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4905..4946
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4967..5534
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4984..5536
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5128..5145)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5355..5374
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5548..5550,5551..5553,5554..6267)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5548..5550,5551..5553,5554..6264)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5593..5614)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5854..5873)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6201..6222
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6281..6869
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6334..6354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6752..6763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6944..7177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7296..7314)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7382..7486
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7487..7555,7556..8347)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7705..7724)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8518..9106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9007..9026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9260..9277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9517..9538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9553..9558,9559..9576,9577..9583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9591..9607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9596..9618
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9615..9631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9615..9631
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9681..9700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9892..10525
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10572..10697
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11194..11427
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11612..11656
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
       61 acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt
      121 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      181 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      241 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc
      301 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      361 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      421 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      481 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      541 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      601 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
      661 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
      721 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
      781 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
      841 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
      901 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
      961 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     1021 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     1081 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     1141 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     1201 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     1261 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     1321 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     1381 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     1441 gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag
     1501 tttgtacaaa aaagcaggca ccatgcctcc aagaccatca tcaggtgaac tgtggggcat
     1561 ccacttgatg cccccaagaa tcctagtaga atgtttacta ccaaatggaa tgatagtgac
     1621 tttagaatgc ctccgtgagg ctacattaat aaccataaag catgaactat ttaaagaagc
     1681 aagaaaatac cccctccatc aacttcttca agatgaatct tcttacattt tcgtaagtgt
     1741 tactcaagaa gcagaaaggg aagaattttt tgatgaaaca agacgacttt gtgaccttcg
     1801 gctttttcaa ccctttttaa aagtaattga accagtaggc aaccgtgaag aaaagatcct
     1861 caatcgagaa attggttttg ctatcggcat gccagtgtgt gaatttgata tggttaaaga
     1921 tccagaagta caggacttcc gaagaaatat tctgaacgtt tgtaaagaag ctgtggatct
     1981 tagggacctc aattcacctc atagtagagc aatgtatgtc tatcctccaa atgtagaatc
     2041 ttcaccagaa ttgccaaagc acatatataa taaattagat aaagggcaaa taatagtggt
     2101 gatctgggta atagtttctc caaataatga caagcagaag tatactctga aaatcaacca
     2161 tgactgtgta ccagaacaag taattgctga agcaatcagg aaaaaaactc gaagtatgtt
     2221 gctatcctct gaacaactaa aactctgtgt tttagaatat cagggcaagt atattttaaa
     2281 agtgtgtgga tgtgatgaat acttcctaga aaaatatcct ctgagtcagt ataagtatat
     2341 aagaagctgt ataatgcttg ggaggatgcc caatttgatg ttgatggcta aagaaagcct
     2401 ttattctcaa ctgccaatgg actgttttac aatgccatct tattccagac gcatttccac
     2461 agctacacca tatatgaatg gagaaacatc tacaaaatcc ctttgggtta taaatagtgc
     2521 actcagaata aaaattcttt gtgcaaccta cgtgaatgta aatattcgag acattgataa
     2581 gatctatgtt cgaacaggta tctaccatgg aggagaaccc ttatgtgaca atgtgaacac
     2641 tcaaagagta ccttgttcca atcccaggtg gaatgaatgg ctgaattatg atatatacat
     2701 tcctgatctt cctcgtgctg ctcgactttg cctttccatt tgctctgtta aaggccgaaa
     2761 gggtgctaaa gaggaacact gtccattggc atggggaaat ataaacttgt ttgattacac
     2821 agacactcta gtatctggaa aaatggcttt gaatctttgg ccagtacctc atggattaga
     2881 agatttgctg aaccctattg gtgttactgg atcaaatcca aataaagaaa ctccatgctt
     2941 agagttggag tttgactggt tcagcagtgt ggtaaagttc ccagatatgt cagtgattga
     3001 agagcatgcc aattggtctg tatcccgaga agcaggattt agctattccc acgcaggact
     3061 gagtaacaga ctagctagag acaatgaatt aagggaaaat gacaaagaac agctcaaagc
     3121 aatttctaca cgagatcgtc tctctgaaat cactgagcag gagaaagatt ttctatggag
     3181 tcacagacac tattgtgtaa ctatccccga aattctaccc aaattgcttc tgtctgttaa
     3241 atggaattct agagatgaag tagcccagat gtattgcttg gtaaaagatt ggcctccaat
     3301 caaacctgaa caggctatgg aacttctgga ctgtaattac ccagatccta tggttcgagg
     3361 ttttgctgtt cggtgcttgg aaaaatattt aacagatgac aaactttctc agtatttaat
     3421 tcagctagta caggtcctaa aatatgaaca atatttggat aacttgcttg tgagattttt
     3481 actgaagaaa gcattgacta atcaaaggat tgggcacttt ttcttttggc atttaaaatc
     3541 tgagatgcac aataaaacag ttagccagag gtttggcctg cttttggagt cctattgtcg
     3601 tgcatgtggg atgtatttga agcacctgaa taggcaagtc gaggcaatgg aaaagctcat
     3661 taacttaact gacattctca aacaggagaa gaaggatgaa acacaaaagg tacagatgaa
     3721 gtttttagtt gagcaaatga ggcgaccaga tttcatggat gctctacagg gctttctgtc
     3781 tcctctaaac cctgctcatc aactaggaaa cctcaggctt gaagagtgtc gaattatgtc
     3841 ctctgcaaaa aggccactgt ggttgaattg ggagaaccca gacatcatgt cagagttact
     3901 gtttcagaac aatgagatca tctttaaaaa tggggatgat ttacggcaag atatgctaac
     3961 acttcaaatt attcgtatta tggaaaatat ctggcaaaat caaggtcttg atcttcgaat
     4021 gttaccttat ggttgtctgt caatcggtga ctgtgtggga cttattgagg tggtgcgaaa
     4081 ttctcacact attatgcaaa ttcagtgcaa aggcggcttg aaaggtgcac tgcagttcaa
     4141 cagccacaca ctacatcagt ggctcaaaga caagaacaaa ggagaaatat atgatgcagc
     4201 cattgacctg tttacacgtt catgtgctgg atactgtgta gctaccttca ttttgggaat
     4261 tggagatcgt cacaatagta acatcatggt gaaagacgat ggacaactgt ttcatataga
     4321 ttttggacac tttttggatc acaagaagaa aaaatttggt tataaacgag aacgtgtgcc
     4381 atttgttttg acacaggatt tcttaatagt gattagtaaa ggagcccaag aatgcacaaa
     4441 gacaagagaa tttgagaggt ttcaggagat gtgttacaag gcttatctag ctattcgaca
     4501 gcatgccaat ctcttcataa atcttttctc aatgatgctt ggctctggaa tgccagaact
     4561 acaatctttt gatgacattg catacattcg aaagacccta gccttagata aaactgagca
     4621 agaggctttg gagtatttca tgaaacaaat gaatgatgca catcatggtg gctggacaac
     4681 aaaaatggat tggatcttcc acacaattaa acagcatgca ttgaactaga acccagcttt
     4741 cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gtttaagtga gttatagaag
     4801 tagtacccta tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc
     4861 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     4921 accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc
     4981 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5041 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5101 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5161 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5221 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5281 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5341 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5401 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5461 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5521 aaacacgatg ataatatggc cacacatatg gtgagcaagg gcgaggagct gttcaccggg
     5581 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     5641 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5701 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5761 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5821 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5881 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5941 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6001 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6061 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6121 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     6181 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6241 ctcggcatgg acgagctgta caagtaaatc gatagatcct aatcaacctc tggattacaa
     6301 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6361 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6421 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6481 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6541 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6661 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6721 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6781 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6841 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     6901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6961 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7141 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7201 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     7261 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     7321 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7381 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7441 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7501 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7561 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7621 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7681 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     7741 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7801 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7861 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7921 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7981 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8041 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8101 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8161 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8221 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8281 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8341 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8401 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8461 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8521 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8581 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8641 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8701 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8761 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8821 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8881 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8941 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9001 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9061 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9121 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9181 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9241 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9301 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     9361 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9421 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     9481 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9541 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9601 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
     9661 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9721 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9781 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9841 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     9901 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     9961 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    10021 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    10081 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    10141 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    10201 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
    10261 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
    10321 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10381 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10441 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10501 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10561 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10621 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10681 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10741 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10801 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10861 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10921 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10981 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    11041 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    11101 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    11161 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    11221 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    11281 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    11341 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    11401 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    11461 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    11521 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    11581 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11641 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    11701 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11761 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaa
//
