LOCUS       sequence-237720-       11700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CB D1067Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CB-D1067Y
FEATURES             Location/Qualifiers
     source          1..11700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    85..202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        250..1428
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          481..1419
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1376..1396
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1448..1472
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1683..1691
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2990..3001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4687..4711)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4719..4737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4762..4781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4763..4781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4787..4807)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4861..4902
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4923..5490
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4940..5492
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5084..5101)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5311..5330
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5504..6103
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5504..5523)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6000..6020
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6117..6705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6170..6190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6588..6599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6780..7013
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7132..7150)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7218..7322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7323..7391,7392..8183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7541..7560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8354..8942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8843..8862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9096..9113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9353..9374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9389..9394,9395..9412,9413..9419)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9427..9443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9432..9454
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9451..9467
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9451..9467
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9517..9536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9728..10361
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10408..10533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11030..11263
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11448..11492
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc
       61 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
      121 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      181 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac
      241 tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      301 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      361 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      421 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      481 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
      541 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      601 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      661 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      721 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      781 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      841 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      901 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      961 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     1021 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     1081 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     1141 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     1201 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1261 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1321 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1381 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccctgca
     1441 gatatcaaca agtttgtaca aaaaagcagg caccatgtgc ttcagtttca taatgcctcc
     1501 tgctatggca gacatccttg acatctgggc ggtggattca cagatagcat ctgatggctc
     1561 catacctgtg gatttccttt tgcccactgg gatttatatc cagttggagg tacctcggga
     1621 agctaccatt tcttatatta agcagatgtt atggaagcaa gttcacaatt acccaatgtt
     1681 caacctcctt atggatattg actcctatat gtttgcatgt gtgaatcaga ctgctgtata
     1741 tgaggagctt gaagatgaaa cacgaagact ctgtgatgtc agaccttttc ttccagttct
     1801 caaattagtg acaagaagtt gtgacccagg ggaaaaatta gactcaaaaa ttggagtcct
     1861 tataggaaaa ggtctgcatg aatttgattc cttgaaggat cctgaagtaa atgaatttcg
     1921 aagaaaaatg cgcaaattca gcgaggaaaa aatcctgtca cttgtgggat tgtcttggat
     1981 ggactggcta aaacaaacat atccaccaga gcatgaacca tccatccctg aaaacttaga
     2041 agataaactt tatgggggaa agctcatcgt agctgttcat tttgaaaact gccaggacgt
     2101 gtttagcttt caagtgtctc ctaatatgaa tcctatcaaa gtaaatgaat tggcaatcca
     2161 aaaacgtttg actattcatg ggaaggaaga tgaagttagc ccctatgatt atgtgttgca
     2221 agtcagcggg agagtagaat atgtttttgg tgatcatcca ctaattcagt tccagtatat
     2281 ccggaactgt gtgatgaaca gagccctgcc ccattttata cttgtggaat gctgcaagat
     2341 caagaaaatg tatgaacaag aaatgattgc catagaggct gccataaatc gaaattcatc
     2401 taatcttcct cttccattac caccaaagaa aacacgaatt atttctcatg tttgggaaaa
     2461 taacaaccct ttccaaattg tcttggttaa gggaaataaa cttaacacag aggaaactgt
     2521 aaaagttcat gtcagggctg gtctttttca tggtactgag ctcctgtgta aaaccatcgt
     2581 aagctcagag gtatcaggga aaaatgatca tatttggaat gaaccactgg aatttgatat
     2641 taatatttgt gacttaccaa gaatggctcg attatgtttt gctgtttatg cagttttgga
     2701 taaagtaaaa acgaagaaat caacgaaaac tattaatccc tctaaatatc agaccatcag
     2761 gaaagctgga aaagtgcatt atcctgtagc gtgggtaaat acgatggttt ttgactttaa
     2821 aggacaattg agaactggag acataatatt acacagctgg tcttcatttc ctgatgaact
     2881 cgaagaaatg ttgaatccaa tgggaactgt tcaaacaaat ccatatactg aaaatgcaac
     2941 agctttgcat gttaaatttc cagagaataa aaaacaacct tattattacc ctcccttcga
     3001 taagattatt gaaaaggcag ctgagattgc aagcagtgat agtgctaatg tgtcaagtcg
     3061 aggtggaaaa aagtttcttc ctgtattgaa agaaatcttg gacagggatc ccttgtctca
     3121 actgtgtgaa aatgaaatgg atcttatttg gactttgcga caagactgcc gagagatttt
     3181 cccacaatca ctgccaaaat tactgctgtc aatcaagtgg aataaacttg aggatgttgc
     3241 tcagcttcag gcgctgcttc agatttggcc taaactgccc ccccgggagg ccctagagct
     3301 tctggatttc aactatccag accagtacgt tcgagaatat gctgtaggct gcctgcgaca
     3361 gatgagtgat gaagaacttt ctcaatatct tttacaactg gtgcaagtgt taaaatatga
     3421 gccttttctt gattgtgccc tctctagatt cctattagaa agagcacttg gtaatcggag
     3481 gatagggcag tttctatttt ggcatcttag gtcagaagtg cacattcctg ctgtctcagt
     3541 acaatttggt gtcatccttg aagcatactg ccggggaagt gtggggcaca tgaaagtgct
     3601 ttctaagcag gttgaagcac tcaataagtt aaaaacttta aatagtttaa tcaaactgaa
     3661 tgccgtgaag ttaaacagag ccaaagggaa ggaggccatg catacctgtt taaaacagag
     3721 tgcttaccgg gaagccctct ctgacctgca gtcacccctg aacccatgtg ttatcctctc
     3781 agaactctat gttgaaaagt gcaaatacat ggattccaaa atgaagcctt tgtggctggt
     3841 atacaataac aaggtatttg gtgaggattc agttggagtg atttttaaaa atggtgatga
     3901 tttacgacag gatatgttga cactccaaat gttgcgcttg atggatttac tctggaaaga
     3961 agctggtttg gatcttcgga tgttgcctta tggctgttta gcaacaggag atcgctctgg
     4021 cctcattgaa gttgtgagca cctctgaaac aattgctgac attcagctga acagtagcaa
     4081 tgtggctgct gcagcagcct tcaacaaaga tgcccttctg aactggctta aagaatacaa
     4141 ctctggggat gacctggacc gagccattga ggaatttaca ctgtcctgtg ctggctactg
     4201 tgtagcttct tatgtccttg ggattggtga cagacatagt gacaacatca tggtcaaaaa
     4261 aactggccag ctcttccaca ttgactttgg acatattctt ggaaatttca aatctaagtt
     4321 tggcattaaa agggagcgag tgccttttat tcttacctat gatttcatcc atgtcattca
     4381 acaaggaaaa acaggaaata cagaaaagtt tggccggttc cgccagtgtt gtgaggatgc
     4441 atatctgatt ttacgacggc atgggaatct cttcatcact ctctttgcgc tgatgttgac
     4501 tgcagggctt cctgaactca catcagtcaa agatatacag tatcttaagg actctcttgc
     4561 attagggaag agtgaagaag aagcactcaa acagtttaag caaaaatttg atgaggcgct
     4621 cagggaaagc tggactacta aagtgaactg gatggcccac acagttcgga aatactacag
     4681 atcttaaccc aactttcttg tacaaagtgg tgatatccaa ttaaccctca ctaaagggaa
     4741 gatattgaaa gaatttgatg tccctatagt gagtcgtatt accacccaac ttttctatac
     4801 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     4861 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
     4921 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4981 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5041 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5101 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5161 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     5221 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     5281 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     5341 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     5401 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     5461 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc
     5521 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5581 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5641 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5701 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5761 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5821 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5881 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5941 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6001 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6061 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc
     6121 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6181 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6241 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6301 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6361 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6421 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6481 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6541 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6601 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6661 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     6721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6781 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7021 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7081 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     7141 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7561 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
     9181 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9241 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     9301 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9361 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9421 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     9481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9541 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     9601 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     9661 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9721 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     9781 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     9841 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     9901 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     9961 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    10021 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    10081 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    10141 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    10201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    10681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    10741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    10801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    10861 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10921 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10981 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    11041 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    11101 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    11161 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    11221 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    11281 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    11341 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    11401 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    11461 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    11521 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    11581 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    11641 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
//
