LOCUS       sequence-237726-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00094821
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(278..294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(278..294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(291..313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(326..332,333..350,351..356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    371..392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(509..526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(760..779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta agctcatctt ctagcagcag cagcagctgg tggatttctc aaagtagaag
       61 aaacagtaaa tttttcaact tgagtccaat gagtaatatt acaattatga tcaacagtaa
      121 tagaaatttg atcagtaatc aaattaaaag ttttagcagt agatggaccc atagtaccag
      181 tttcaacaac agcaccttca aatctaataa caatatcaca aatagacatt agagaccaag
      241 ttcatcccaa tggcgcgccg agcttggctc gagcatggtc atagctgttt cctgtgtgaa
      301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgt
//
