LOCUS       sequence-237727-        9724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PTEN G127R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PTEN-G127R
FEATURES             Location/Qualifiers
     source          1..9724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    139..256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        304..1482
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          535..1473
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1430..1450
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1502..1526
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2742..2766)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2819..2860
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2881..3448
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2898..3450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3042..3059)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3269..3288
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3462..3464,3465..3467,3468..4181)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3462..3464,3465..3467,3468..4178)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3507..3528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3768..3787)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4115..4136
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4195..4783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4248..4268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4666..4677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4858..5091
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5210..5228)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5296..5400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5401..5469,5470..6261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5619..5638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6432..7020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6921..6940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7174..7191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7431..7452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7467..7472,7473..7490,7491..7497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7505..7521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7510..7532
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7529..7545
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7529..7545
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7595..7614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7806..8439
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8486..8611
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9108..9341
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9526..9570
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
       61 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat
      121 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      181 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      241 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      301 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      361 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      421 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      481 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      541 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      601 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      661 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      721 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      781 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      841 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      901 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      961 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     1021 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     1081 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1141 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1201 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1261 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1321 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1381 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1441 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccc tgcagatatc
     1501 aacaagtttg tacaaaaaag caggcaccat gacagccatc atcaaagaga tcgttagcag
     1561 aaacaaaagg agatatcaag aggatggatt cgacttagac ttgacctata tttatccaaa
     1621 cattattgct atgggatttc ctgcagaaag acttgaaggc gtatacagga acaatattga
     1681 tgatgtagta aggtttttgg attcaaagca taaaaaccat tacaagatat acaatctttg
     1741 tgctgaaaga cattatgaca ccgccaaatt taattgcaga gttgcacaat atccttttga
     1801 agaccataac ccaccacagc tagaacttat caaacccttt tgtgaagatc ttgaccaatg
     1861 gctaagtgaa gatgacaatc atgttgcagc aattcactgt aaagctagaa agggacgaac
     1921 tggtgtaatg atatgtgcat atttattaca tcggggcaaa tttttaaagg cacaagaggc
     1981 cctagatttc tatggggaag taaggaccag agacaaaaag ggagtaacta ttcccagtca
     2041 gaggcgctat gtgtattatt atagctacct gttaaagaat catctggatt atagaccagt
     2101 ggcactgttg tttcacaaga tgatgtttga aactattcca atgttcagtg gcggaacttg
     2161 caatcctcag tttgtggtct gccagctaaa ggtgaagata tattcctcca attcaggacc
     2221 cacacgacgg gaagacaagt tcatgtactt tgagttccct cagccgttac ctgtgtgtgg
     2281 tgatatcaaa gtagagttct tccacaaaca gaacaagatg ctaaaaaagg acaaaatgtt
     2341 tcacttttgg gtaaatacat tcttcatacc aggaccagag gaaacctcag aaaaagtaga
     2401 aaatggaagt ctatgtgatc aagaaatcga tagcatttgc agtatagagc gtgcagataa
     2461 tgacaaggaa tatctagtac ttactttaac aaaaaatgat cttgacaaag caaataaaga
     2521 caaagccaac cgatactttt ctccaaattt taaggtgaag ctgtacttca caaaaacagt
     2581 agaggagccg tcaaatccag aggctagcag ttcaacttct gtaacaccag atgttagtga
     2641 caatgaacct gatcattata gatattctga caccactgac tctgatccag agaatgaacc
     2701 ttttgatgaa gatcagcata cacaaattac aaaagtctag aacccagctt tcttgtacaa
     2761 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     2821 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga
     2881 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     2941 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3001 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3061 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3121 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3181 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3241 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3301 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3361 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3421 ggttttcctt tgaaaaacac gatgataata tggccacaca tatggtgagc aagggcgagg
     3481 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3541 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3601 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3661 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3721 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3781 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3841 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3901 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3961 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4021 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4081 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4141 ccgccgggat cactctcggc atggacgagc tgtacaagta aatcgataga tcctaatcaa
     4201 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4261 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4321 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4381 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4441 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4501 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4561 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4621 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4681 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4741 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     4801 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4861 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4921 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4981 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5041 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5101 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5161 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     5221 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5641 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5941 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6001 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6121 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6241 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6541 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7201 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     7261 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7321 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     7381 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7441 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7501 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     7561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7621 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     7681 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     7741 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     7801 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     7861 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7921 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7981 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8041 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8101 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8161 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8221 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     8461 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     8521 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     8581 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     8641 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     8701 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     8761 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     8821 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8881 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8941 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9001 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9061 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9121 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9181 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9241 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9301 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9361 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9421 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9481 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9541 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9601 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9661 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9721 cacc
//
