LOCUS       sequence-237730-       11798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CG R544*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CG-R544*
FEATURES             Location/Qualifiers
     source          1..11798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    118..235
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        283..1461
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          514..1452
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1409..1429
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1481..1505
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4414..4425
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4818..4842)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4850..4868
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4893..4912)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4894..4912)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4918..4938)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4992..5033
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5054..5621
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5071..5623
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5215..5232)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5442..5461
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5635..6234
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5635..5654)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6131..6151
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6248..6836
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6301..6321)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6719..6730)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6911..7144
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7263..7281)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7349..7453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7454..7522,7523..8314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7672..7691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8485..9073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8974..8993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9227..9244
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9484..9505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9520..9525,9526..9543,9544..9550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9558..9574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9563..9585
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9582..9598
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9582..9598
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9648..9667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9859..10492
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10539..10664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11161..11394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11579..11623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
       61 tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt
      121 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      181 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      241 ttattacagg gacagcagag atccagtttg gactagtcgt gaggctccgg tgcccgtcag
      301 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      361 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      421 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      481 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
      541 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
      601 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
      661 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
      721 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
      781 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
      841 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
      901 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
      961 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     1021 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     1081 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     1141 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     1201 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     1261 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     1321 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     1381 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     1441 cttccatttc aggtgtcgtg aagcggccct gcagatatca acaagtttgt acaaaaaagc
     1501 aggcaccatg gagctggaga actataaaca gcccgtggtg ctgagagagg acaactgccg
     1561 aaggcgccgg aggatgaagc cgcgcagtgc tgcggccagc ctgtcctcca tggagctcat
     1621 ccccatcgag ttcgtgctgc ccaccagcca gcgcaaatgc aagagccccg aaacggcgct
     1681 gctgcacgtg gccggccacg gcaacgtgga gcagatgaag gcccaggtgt ggctgcgagc
     1741 gctggagacc agcgtggcgg cggacttcta ccaccggctg ggaccgcatc acttcctcct
     1801 gctctatcag aagaaggggc agtggtacga gatctacgac aagtaccagg tggtgcagac
     1861 tctggactgc ctgcgctact ggaaggccac gcaccggagc ccgggccaga tccacctggt
     1921 gcagcggcac ccgccctccg aggagtccca agccttccag cggcagctca cggcgctgat
     1981 tggctatgac gtcactgacg tcagcaacgt gcacgacgat gagctggagt tcacgcgccg
     2041 tggcttggtg accccgcgca tggcggaggt ggccagccgc gaccccaagc tctacgccat
     2101 gcacccgtgg gtgacgtcca agcccctccc ggagtacctg tggaagaaga ttgccaacaa
     2161 ctgcatcttc atcgtcattc accgcagcac caccagccag accattaagg tctcacccga
     2221 cgacaccccc ggcgccatcc tgcagagctt cttcaccaag atggccaaga agaaatctct
     2281 gatggatatt cccgaaagcc aaagcgaaca ggattttgtg ctgcgcgtct gtggccggga
     2341 tgagtacctg gtgggcgaaa cgcccatcaa aaacttccag tgggtgaggc actgcctcaa
     2401 gaacggagaa gagattcacg tggtactgga cacgcctcca gacccggccc tagacgaggt
     2461 gaggaaggaa gagtggccgc tggtggatga ctgcacggga gtcaccggct accatgagca
     2521 gcttaccatc cacggcaagg accacgagag tgtgttcacc gtgtccctgt gggactgcga
     2581 ccgcaagttc agggtcaaga tcagaggcat tgatatcccc gtcctgcctc ggaacaccga
     2641 cctcacagtt tttgtagagg caaacatcca gcatgggcaa caagtccttt gccaaaggag
     2701 aaccagcccc aaacccttca cagaggaggt gctgtggaat gtgtggcttg agttcagtat
     2761 caaaatcaaa gacttgccca aaggggctct actgaacctc cagatctact gcggtaaagc
     2821 tccagcactg tccagcaagg cctctgcaga gtcccccagt tctgagtcca agggcaaagt
     2881 tcagcttctc tattatgtga acctgctgct gatagaccac cgtttcctcc tgcgccgtgg
     2941 agaatacgtc ctccacatgt ggcagatatc tgggaaggga gaagaccaag gaagcttcaa
     3001 tgctgacaaa ctcacgtctg caactaaccc agacaaggag aactcaatgt ccatctccat
     3061 tcttctggac aattactgcc acccgatagc cctgcctaag catcagccca cccctgaccc
     3121 ggaaggggac cgggtttgag cagaaatgcc caaccagctt cgcaagcaat tggaggcgat
     3181 catagccact gatccactta accctctcac agcagaggac aaagaattgc tctggcattt
     3241 tagatacgaa agccttaagc acccaaaagc atatcctaag ctatttagtt cagtgaaatg
     3301 gggacagcaa gaaattgtgg ccaaaacata ccaattgttg gccagaaggg aagtctggga
     3361 tcaaagtgct ttggatgttg ggttaacaat gcagctcctg gactgcaact tctcagatga
     3421 aaatgtaaga gccattgcag ttcagaaact ggagagcttg gaggacgatg atgttctgca
     3481 ttaccttcta caattggtcc aggctgtgaa atttgaacca taccatgata gcgcccttgc
     3541 cagatttctg ctgaagcgtg gtttaagaaa caaaagaatt ggtcactttt tgttttggtt
     3601 cttgagaagt gagatagccc agtccagaca ctatcagcag aggttcgctg tgattctgga
     3661 agcctatctg aggggctgtg gcacagccat gctgcacgac tttacccaac aagtccaagt
     3721 aatcgagatg ttacaaaaag tcacccttga tattaaatcg ctctctgctg aaaagtatga
     3781 cgtcagttcc caagttattt cacaacttaa acaaaagctt gaaaacctgc agaattctca
     3841 actccccgaa agctttagag ttccatatga tcctggactg aaagcaggag cgctggcaat
     3901 tgaaaaatgt aaagtaatgg cctccaagaa aaaaccacta tggcttgagt ttaaatgtgc
     3961 cgatcctaca gccctatcaa atgaaacaat tggaattatc tttaaacatg gtgatgatct
     4021 gcgccaagac atgcttattt tacagattct acgaatcatg gagtctattt gggagactga
     4081 atctttggat ctatgcctcc tgccatatgg ttgcatttca actggtgaca aaataggaat
     4141 gatcgagatt gtgaaagacg ccacgacaat tgccaaaatt cagcaaagca cagtgggcaa
     4201 cacgggagca tttaaagatg aagtcctgaa tcactggctc aaagaaaaat cccctactga
     4261 agaaaagttt caggcagcag tggagagatt tgtttattcc tgtgcaggct actgtgtggc
     4321 aacctttgtt cttggaatag gcgacagaca caatgacaat attatgatca ccgagacagg
     4381 aaacctattt catattgact tcgggcacat tcttgggaat tacaaaagtt tcctgggcat
     4441 taataaagag agagtgccat ttgtgctaac ccctgacttc ctctttgtga tgggaacttc
     4501 tggaaagaag acaagcccac acttccagaa atttcaggac atctgtgtta aggcttatct
     4561 agcccttcgt catcacacaa acctactgat catcctgttc tccatgatgc tgatgacagg
     4621 aatgccccag ttaacaagca aagaagacat tgaatatatc cgggatgccc tcacagtggg
     4681 gaaaaatgag gaggatgcta aaaagtattt tcttgatcag atcgaagttt gcagagacaa
     4741 aggatggact gtgcagttta attggtttct acatcttgtt cttggcatca aacaaggaga
     4801 gaaacattca gcctagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc
     4861 actaaaggga taagaaagtg aaatgtaaat tgccctatag tgagtcgtat taccacccaa
     4921 cttttctata caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     4981 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     5041 gttagtaatg agatccctcc cccccccnta acgttactgg ccgaagccgc ttggaataag
     5101 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5161 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5221 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5281 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5341 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5401 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5461 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5521 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5581 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc
     5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5821 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6001 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat
     6241 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6661 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     6841 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6901 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6961 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7021 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7081 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7141 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7201 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     7261 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7321 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7381 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7441 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7501 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7561 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7621 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7681 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     7741 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7801 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7861 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7921 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7981 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8041 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8101 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8161 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8221 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8281 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8341 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8401 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8461 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9001 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9061 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9241 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9301 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9421 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9481 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9541 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     9601 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9661 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     9721 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     9781 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     9841 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     9901 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     9961 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
    10021 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
    10081 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
    10141 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
    10201 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
    10261 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
    10321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    10501 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    10561 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    10621 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    10681 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    10741 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    10801 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    10861 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    10921 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    10981 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
    11041 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    11101 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    11161 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    11221 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    11281 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    11341 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    11401 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    11461 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    11521 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    11581 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    11641 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    11701 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    11761 atcgtttcag acccacctcc caaccccgag gggacccg
//
