LOCUS       sequence-237733-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PTPN11 T73I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PTPN11-T73I
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    171..288
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        336..1514
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          567..1505
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1462..1482
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1534..1558
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2945..2969)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2977..2995
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3020..3039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3021..3039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3045..3065)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3119..3160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3181..3748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3198..3750
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3342..3359)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3569..3588
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3762..4361
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3762..3781)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4258..4278
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4375..4963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4428..4448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4846..4857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5038..5271
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5390..5408)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5476..5580
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5581..5649,5650..6441)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5799..5818)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6612..7200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7101..7120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7354..7371
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7611..7632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7647..7652,7653..7670,7671..7677)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7685..7701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7690..7712
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7709..7725
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7709..7725
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7775..7794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7986..8619
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8666..8791
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9288..9521
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9706..9750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
       61 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
      121 cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga
      181 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      241 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      301 agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt cagtgggcag
      361 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      421 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      481 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      541 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      601 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      661 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      721 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      781 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      841 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      901 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      961 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     1021 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     1081 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     1141 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     1201 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1261 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1321 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1381 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1441 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1501 ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa agcaggcacc
     1561 atgacatcgc ggagatggtt tcacccaaat atcactggtg tggaggcaga aaacctactg
     1621 ttgacaagag gagttgatgg cagttttttg gcaaggccta gtaaaagtaa ccctggagac
     1681 ttcacacttt ccgttagaag aaatggagct gtcacccaca tcaagattca gaacactggt
     1741 gattactatg acctgtatgg aggggagaaa tttgccattt tggctgagtt ggtccagtat
     1801 tacatggaac atcacgggca attaaaagag aagaatggag atgtcattga gcttaaatat
     1861 cctctgaact gtgcagatcc tacctctgaa aggtggtttc atggacatct ctctgggaaa
     1921 gaagcagaga aattattaac tgaaaaagga aaacatggta gttttcttgt acgagagagc
     1981 cagagccacc ctggagattt tgttctttct gtgcgcactg gtgatgacaa aggggagagc
     2041 aatgacggca agtctaaagt gacccatgtt atgattcgct gtcaggaact gaaatacgac
     2101 gttggtggag gagaacggtt tgattctttg acagatcttg tggaacatta taagaagaat
     2161 cctatggtgg aaacattggg tacagtacta caactcaagc agccccttaa cacgactcgt
     2221 ataaatgctg ctgaaataga aagcagagtt cgagaactaa gcaaattagc tgagaccaca
     2281 gataaagtca aacaaggctt ttgggaagaa tttgagacac tacaacaaca ggagtgcaaa
     2341 cttctctaca gccgaaaaga gggtcaaagg caagaaaaca aaaacaaaaa tagatataaa
     2401 aacatcctgc cctttgatca taccagggtt gtcctacacg atggtgatcc caatgagcct
     2461 gtttcagatt acatcaatgc aaatatcatc atgcctgaat ttgaaaccaa gtgcaacaat
     2521 tcaaagccca aaaagagtta cattgccaca caaggctgcc tgcaaaacac ggtgaatgac
     2581 ttttggcgga tggtgttcca agaaaactcc cgagtgattg tcatgacaac gaaagaagtg
     2641 gagagaggaa agagtaaatg tgtcaaatac tggcctgatg agtatgctct aaaagaatat
     2701 ggcgtcatgc gtgttaggaa cgtcaaagaa agcgccgctc atgactatac gctaagagaa
     2761 cttaaacttt caaaggttgg acaagggaat acggagagaa cggtctggca ataccacttt
     2821 cggacctggc cggaccacgg cgtgcccagc gaccctgggg gcgtgctgga cttcctggag
     2881 gaggtgcacc ataagcagga gagcatcatg gatgcagggc cggtcgtggt gcactgcagg
     2941 tagaacccag ctttcttgta caaagtggtg atatccaatt aaccctcact aaaggaagat
     3001 gatagttaag tgtaagttac cctatagtga gtcgtattac cacccaactt ttctatacaa
     3061 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     3121 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga
     3181 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3241 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3301 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3361 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3421 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3481 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3541 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3601 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3661 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3721 ggttttcctt tgaaaaacac gatgataata tggccacaca tatgaccgag tacaagccca
     3781 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     3841 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     3901 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     3961 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     4021 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     4081 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     4141 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     4201 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     4261 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     4321 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta aatcgataga tcctaatcaa
     4381 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4441 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4501 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4561 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4621 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4681 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4741 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4801 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4861 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4921 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     4981 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5041 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     5101 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5161 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5221 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5281 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5341 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     5401 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5461 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5521 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5581 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5641 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5701 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5761 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5821 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5881 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5941 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6001 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6061 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6121 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6181 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6241 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6301 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6361 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6421 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6481 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6541 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6601 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6661 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6721 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6781 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6841 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6901 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6961 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7021 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7081 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7141 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7201 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7261 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7321 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7381 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     7441 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7501 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     7561 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7621 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7681 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     7741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7801 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     7861 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     7921 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     7981 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     8041 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     8101 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8161 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8221 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8281 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8341 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8401 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     8641 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     8701 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     8761 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     8821 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     8881 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     8941 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     9001 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     9061 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     9121 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9181 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9241 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9301 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9361 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9421 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9481 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9541 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9601 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9661 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9721 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9781 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9841 gctgtacttt ctatagtgaa tagagttagg ca
//
