LOCUS       sequence-237734-        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SIRPB1 E170K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-SIRPB1-E170K
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    157..274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        322..1500
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          553..1491
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1448..1468
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1520..1544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2769..2793)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2801..2819
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2844..2863)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2845..2863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2869..2889)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2943..2984
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3005..3572
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3022..3574
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3166..3183)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3393..3412
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3586..3588,3589..3591,3592..4305)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3586..3588,3589..3591,3592..4302)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3631..3652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3892..3911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4239..4260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4319..4907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4372..4392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4790..4801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4982..5215
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5334..5352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5420..5524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5525..5593,5594..6385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5743..5762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6556..7144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7045..7064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7298..7315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7555..7576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7591..7596,7597..7614,7615..7621)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7629..7645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7634..7656
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7653..7669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7653..7669
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7719..7738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7930..8563
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8610..8735
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9232..9465
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9650..9694
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
       61 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
      121 atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      181 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      241 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      301 tccagtttgg actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc
      361 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
      421 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
      481 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
      541 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
      601 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
      661 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
      721 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
      781 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
      841 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
      901 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
      961 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     1021 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     1081 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     1141 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     1201 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     1261 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     1321 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     1381 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     1441 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     1501 agcggccctg cagatatcaa caagtttgta caaaaaagca gaagggccgt caaggcccac
     1561 catgcccgtg ccagcctcct ggccccacct tcctagtcct ttcctgctga tgacgctact
     1621 gctggggaga ctcacaggag tggcaggtga ggacgagcta caggtgattc agcctgaaaa
     1681 gtccgtatca gttgcagctg gagagtcggc cactctgcgc tgtgctatga cgtccctgat
     1741 ccctgtgggg cccatcatgt ggtttagagg agctggagca ggccgggaat taatctacaa
     1801 tcagaaagaa ggccacttcc cacgggtaac aactgtttca gaactcacaa agagaaacaa
     1861 cctggacttt tccatcagca tcagtaacat caccccagca gacgccggca cctactactg
     1921 tgtgaagttc cggaaaggga gccctgacga cgtggagttt aagtctggag caggcactga
     1981 gctgtctgtg cgcgccaaac cctctgcccc cgtggtatcg ggccctgcgg tgagggccac
     2041 acctgagcac acagtgagct tcacctgcaa gtcccatggc ttctctccca gagacatcac
     2101 cctgaaatgg ttcaaaaatg ggaatgagct ctcagacttc cagaccaacg tggaccccgc
     2161 aggagacagt gtgtcctaca gcatccacag cacagccagg gtggtgctga cccgtgggga
     2221 cgttcactct caagtcatct gcgagatagc ccacatcacc ttgcaggggg accctcttcg
     2281 tgggactgcc aacttgtctg aggccatccg agttccaccc accttggagg ttactcaaca
     2341 gcccatgagg gcagagaacc aggcaaacgt cacctgccag gtgagcaatt tctacccccg
     2401 gggactacag ctgacctggt tggagaatgg aaatgtgtcc cggacagaaa cagcttcgac
     2461 cctcatagag aacaaggatg gcacctacaa ctggatgagc tggctcctgg tgaacacctg
     2521 tgcccacagg gacgatgtgg tgctcacctg tcaggtggag catgatgggc agcaagcagt
     2581 cagcaaaagc tatgccctgg agatctcagc gcaccagaag gagcacggct cagatatcac
     2641 ccatgaagca gcgctggctc ctactgctcc actcctcgta gctctcctcc tgggccccaa
     2701 gctgctactg gtggttggtg tctctgccat ctacatctgc tggaaacaga aggcctaagg
     2761 cctcatgggc ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt
     2821 taagaagaat tgtatatgag agtccctata gtgagtcgta ttaccaccca acttttctat
     2881 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     2941 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     3001 gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     3061 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     3121 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     3181 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     3241 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     3301 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     3361 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3421 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     3481 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     3541 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt gagcaagggc
     3601 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3661 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3721 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3781 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3841 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3901 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3961 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4021 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4081 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4201 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4261 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga tagatcctaa
     4321 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4381 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4441 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4501 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4561 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4621 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4681 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4741 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4801 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4861 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     4921 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4981 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5041 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5101 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5161 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5221 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5281 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     5341 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5401 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5461 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5521 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5581 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5641 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5701 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5761 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5821 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5881 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5941 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6001 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6061 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6121 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6181 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6241 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6301 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6361 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6421 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6481 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6541 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6601 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6661 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6721 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6781 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6841 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6901 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6961 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7021 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7081 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7141 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7201 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7261 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7321 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7381 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7501 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7561 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7621 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     7681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7741 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7801 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7861 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7921 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7981 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     8041 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     8101 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     8161 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     8221 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     8281 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     8341 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     8401 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8461 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8521 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8581 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8641 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8701 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8761 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8821 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8881 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8941 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9001 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9061 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9121 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9181 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9241 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9301 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9361 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9421 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9481 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9541 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9601 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9661 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9721 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9781 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
//
