LOCUS       sequence-237742-        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SIRPB1 G290E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-SIRPB1-G290E
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    229..346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        394..1572
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          625..1563
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1520..1540
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1592..1616
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2841..2865)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2873..2891
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2916..2935)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2917..2935)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2941..2961)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3015..3056
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3077..3644
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3094..3646
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3238..3255)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3465..3484
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3658..3660,3661..3663,3664..4377)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3658..3660,3661..3663,3664..4374)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3703..3724)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3964..3983)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4311..4332
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4391..4979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4444..4464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4862..4873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5054..5287
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5406..5424)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5492..5596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5597..5665,5666..6457)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5815..5834)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6628..7216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7117..7136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7370..7387
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7627..7648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7663..7668,7669..7686,7687..7693)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7701..7717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7706..7728
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7725..7741
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7725..7741
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7791..7810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8002..8635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8682..8807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9304..9537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9722..9766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
       61 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
      121 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
      181 gatctcgacg gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa
      241 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      301 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      361 ggacagcaga gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag
      421 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
      481 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
      541 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
      601 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
      661 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
      721 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
      781 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
      841 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
      901 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
      961 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     1021 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     1081 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     1141 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     1201 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     1261 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     1321 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     1381 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     1441 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     1501 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     1561 caggtgtcgt gaagcggccc tgcagatatc aacaagtttg tacaaaaaag cagaagggcc
     1621 gtcaaggccc accatgcccg tgccagcctc ctggccccac cttcctagtc ctttcctgct
     1681 gatgacgcta ctgctgggga gactcacagg agtggcaggt gaggacgagc tacaggtgat
     1741 tcagcctgaa aagtccgtat cagttgcagc tggagagtcg gccactctgc gctgtgctat
     1801 gacgtccctg atccctgtgg ggcccatcat gtggtttaga ggagctggag caggccggga
     1861 attaatctac aatcagaaag aaggccactt cccacgggta acaactgttt cagaactcac
     1921 aaagagaaac aacctggact tttccatcag catcagtaac atcaccccag cagacgccgg
     1981 cacctactac tgtgtgaagt tccggaaagg gagccctgac gacgtggagt ttaagtctgg
     2041 agcaggcact gagctgtctg tgcgcgccaa accctctgcc cccgtggtat cgggccctgc
     2101 ggtgagggcc acacctgagc acacagtgag cttcacctgc gagtcccatg gcttctctcc
     2161 cagagacatc accctgaaat ggttcaaaaa tgggaatgag ctctcagact tccagaccaa
     2221 cgtggacccc gcaggagaca gtgtgtccta cagcatccac agcacagcca gggtggtgct
     2281 gacccgtggg gacgttcact ctcaagtcat ctgcgagata gcccacatca ccttgcaggg
     2341 ggaccctctt cgtgggactg ccaacttgtc tgaggccatc cgagttccac ccaccttgga
     2401 ggttactcaa cagcccatga gggcagagaa ccaggcaaac gtcacctgcc aggtgagcaa
     2461 tttctacccc cggggactac agctgacctg gttggagaat gaaaatgtgt cccggacaga
     2521 aacagcttcg accctcatag agaacaagga tggcacctac aactggatga gctggctcct
     2581 ggtgaacacc tgtgcccaca gggacgatgt ggtgctcacc tgtcaggtgg agcatgatgg
     2641 gcagcaagca gtcagcaaaa gctatgccct ggagatctca gcgcaccaga aggagcacgg
     2701 ctcagatatc acccatgaag cagcgctggc tcctactgct ccactcctcg tagctctcct
     2761 cctgggcccc aagctgctac tggtggttgg tgtctctgcc atctacatct gctggaaaca
     2821 gaaggcctaa ggcctcatgg gcccagcttt cttgtacaaa gtggtgatat ccaattaacc
     2881 ctcactaaag ggttagaatg tatatagagt tagatcccta tagtgagtcg tattaccacc
     2941 caacttttct atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg
     3001 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     3061 ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     3121 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3181 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3241 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3301 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3361 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     3421 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3481 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3541 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     3601 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg
     3661 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3721 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3781 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3841 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     3901 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3961 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4021 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4081 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4141 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4201 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4261 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4321 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaatc
     4381 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4441 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4501 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4561 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4621 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4681 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4741 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4801 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4861 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4921 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4981 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5041 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5101 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5161 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5221 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5281 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5341 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     5401 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5461 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5821 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7381 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7441 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7501 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7561 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7621 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7681 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7741 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7801 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     7861 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     7921 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     7981 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     8041 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     8101 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8161 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     8221 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     8281 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8341 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     8401 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     8461 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8521 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8581 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     8641 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     8701 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     8761 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     8821 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8881 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8941 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     9001 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     9061 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     9121 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     9181 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9241 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9301 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9361 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9421 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9481 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     9541 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     9601 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     9661 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9721 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     9781 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
//
