LOCUS       sequence-237744-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BCL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BCL2
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..259
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        307..1485
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          538..1476
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1433..1453
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1505..1529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2253..2277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2330..2371
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2392..2959
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2409..2961
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2553..2570)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2780..2799
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2973..2975,2976..2978,2979..3692)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2973..2975,2976..2978,2979..3689)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3018..3039)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3279..3298)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3626..3647
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3706..4294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3759..3779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4177..4188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4369..4602
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4721..4739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4807..4911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4912..4980,4981..5772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5130..5149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5943..6531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6432..6451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6685..6702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6942..6963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6978..6983,6984..7001,7002..7008)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7016..7032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7021..7043
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7040..7056
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7040..7056
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7106..7125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7317..7950
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7997..8122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8619..8852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9037..9081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
       61 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
      121 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
      181 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
      241 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
      301 tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      361 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
      421 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      481 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
      541 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
      601 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
      661 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
      721 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
      781 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
      841 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
      901 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
      961 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     1021 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     1081 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     1141 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     1201 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     1261 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     1321 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     1381 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     1441 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccctgcagat
     1501 atcaacaagt ttgtacaaaa aagcaggcac catggcgcac gctgggagaa cggggtacga
     1561 taaccgggag atagtgatga agtacatcca ttataagctg tcgcagaggg gctacgagtg
     1621 ggatgcggga gatgtgggcg ccgcgccccc gggggccgcc cccgcaccgg gcatcttctc
     1681 ctcccagccc gggcacacgc cccatccagc cgcatcccgg gacccggtcg ccaggacctc
     1741 gccgctgcag accccggctg cccccggcgc cgccgcgggg cctgcgctca gcccggtgcc
     1801 acctgtggtc cacctgaccc tccgccaggc cggcgacgac ttctcccgcc gctaccgccg
     1861 cgacttcgcc gagatgtcca gccagctgca cctgacgccc ttcaccgcgc ggggacgctt
     1921 tgccacggtg gtggaggagc tcttcaggga cggggtgaac tgggggagga ttgtggcctt
     1981 ctttgagttc ggtggggtca tgtgtgtgga gagcgtcaac cgggagatgt cgcccctggt
     2041 ggacaacatc gccctgtgga tgactgagta cctgaaccgg cacctgcaca cctggatcca
     2101 ggataacgga ggctgggatg cctttgtgga actgtacggc cccagcatgc ggcctctgtt
     2161 tgatttctcc tggctgtctc tgaagactct gctcagtttg gccctggtgg gagcttgcat
     2221 caccctgggt gcctatctgg gccacaagta gaacccagct ttcttgtaca aagtggttga
     2281 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     2341 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc
     2401 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     2461 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     2521 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     2581 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     2641 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     2701 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     2761 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     2821 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     2881 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     2941 ttgaaaaaca cgatgataat atggccacac atatggtgag caagggcgag gagctgttca
     3001 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3061 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3121 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3181 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3241 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3301 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3361 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3421 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3481 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3541 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3601 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3661 tcactctcgg catggacgag ctgtacaagt aaatcgatag atcctaatca acctctggat
     3721 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3781 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3841 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3901 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3961 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4021 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4081 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4141 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4201 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4261 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     4321 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4381 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4441 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4501 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4561 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4621 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     4681 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     4741 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4801 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4861 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4921 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4981 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5041 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5101 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5161 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5221 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5281 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5341 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5401 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5461 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5521 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5581 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5641 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5701 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5761 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5821 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6061 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     6781 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6841 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
     6901 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6961 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7021 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
     7081 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7141 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     7201 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     7261 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     7321 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     7381 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7441 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7501 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7561 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7621 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7681 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7741 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7801 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7861 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7921 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7981 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8041 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8101 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8161 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8221 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8281 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8341 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8401 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8461 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8521 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8581 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8641 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8701 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8761 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8821 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8881 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8941 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9001 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9061 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9121 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9181 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac cc
//
